A new resorbable monofilament suture

Abstract:Bridging nerve gaps by means of autologous nerve grafts involves donor nerve graft harvesting. Recent studies have focused on the use of alternative methods, and one of these is the use of biodegradable nerve guides. After serving their function, nerve guides should degrade to avoid a chronic foreign body reaction. The in vitro degradation, in vitro cytotoxicity, hemocompatibility, and short-term in vivo foreign body reaction of poly( 65 / 35 ( 85 / 15 L / D ) lactide-⑀-caprolactone) nerve guides was studied. The in vitro degradation characteristics of poly(DLLA-⑀-CL) nerve guides were monitored at 2-week time intervals during a period of 22 weeks. Weight loss, degree of swelling of the tube wall, mechanical strength, thermal properties, and the intrinsic viscosity of the nerve guides were determined. Cytotoxicity was studied by measuring the cell proliferation inhibition index (CPII) on mouse fibroblasts in vitro. Cell growth was evaluated by cell counting, while morphology was assessed by light microscopy. Hemocompatibility was evaluated using a thrombin generation assay and a complement convertase assay. The foreign body reaction against poly(DLLA-⑀-CL) nerve guides was investigated by examining toluidine blue stained sections. The in vitro degradation data showed that poly(DLLA-⑀-CL) nerve guides do not swell, maintain their mechanical strength and flexibility for a period of about 8 -10 weeks, and start to lose mass after about 10 weeks. Poly(DLLA-⑀-CL) nerve guides were classified as noncytotoxic, as cytotoxicity tests demonstrated that cell morphology was not affected (CPII 0%). The thrombin generation assay and complement convertase assay indicated that the material is highly hemocompatible. The foreign body reaction against the biomaterial was mild with a light priming of the immunesystem. The results presented in this study demonstrate that poly( 65 / 35 ( 85 / 15 L / D ) lactide-⑀-caprolactone) nerve guides are biocompatible, and show good in vitro degradation characteristics, making these biodegradable nerve guides promising candidates for bridging peripheral nerve defects up to several centimeters.

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“…[32][33][34][35] According to Pitt, 32 the chain degradation can be described by a first order law of the form: …”

Section: Discussionmentioning

confidence: 99%

In vitro degradation and biocompatibility of poly(DL‐lactide‐ϵ‐caprolactone) nerve guides

Meek¹,

Jansen²,

Steendam

et al. 2003

J Biomedical Materials Res

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“…13 Only a few studies have been reported concerning the pH effect on the degradation of suture materials. 14 -17 The present study will describe the strength change of monofilament-type sutures immersed in buffer solutions of different pHs.…”

Section: Introductionmentioning

confidence: 99%

The pH dependence of monofilament sutures on hydrolytic degradation

Tomihata

Suzuki

Ikada

2001

J. Biomed. Mater. Res.

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Hydrolytic degradation of two nonabsorbable sutures, four absorbable sutures, and a new type of absorbable suture was studied in buffered media of various pHs at 37 degrees C. The pH levels fixed in this study were 1.0, 7.4, 8.5, and 10.5. Physical measurements were made on the retention of tensile strength and melting temperature of the sutures after hydrolysis for 12 weeks. Sutures containing glycolic acid as a comonomer exhibited enhanced degradation in alkaline media, similar to polyglycolide multifilament sutures. Poly-p-dioxanone (PDS II) suture lost strength to a significant extent at pH 1.0, suggesting that care should be taken when this suture is used for closing tissues in contact with acidic media, such as the stomach. In marked contrast, the degradation of lactide-epsilon-caprolactone copolymer [P(LA/CL)] suture was not sensitive to the pH of media. The surface morphology of hydrolyzed sutures varied, depending on the pH of media. Particularly, moon-crater-shaped impressions were observed on glycolide-epsilon-caprolactone copolymer (MONOCRYL) and glycolide-trimethylene carbonate-dioxanone copolymer (BIOSYN) sutures. Among the nonabsorbable sutures, nylon (ETHILON) exhibited the fastest loss of strength in acidic buffer solution, and polypropylene (PROLENE) suture retained most of its initial strength at all pHs studied.

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“…This polymer has been used in several medical applications already approved by the US Food and Drug Administration (Bezwada et al, 1995;Darney et al, 1989). Nowadays, PCL is being applied not only in the development of drug delivery systems (Giavaresi et al, 2004), and resorbable sutures (Tomihata et al, 1998), but also as a material for tissue regeneration (Sarasam and Madihally, 2005).…”

Section: Introductionmentioning

confidence: 99%

Development of a new photocrosslinkable biodegradable bioadhesive

Ferreira

Coelho

Gil

2008

International Journal of Pharmaceutics

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Adhesives provide a needle-free method of wound closure and do not require local anaesthetics. Polymeric adhesives have been used for about 3 decades for joining several tissues of the organism. Also, they can accomplish other tasks, such as haemostasis and the ability to seal air leakages and have the potential to serve as delivery systems.PCL was modified with 2-isocyanatoethylmethacrylate to form a macromer that was crosslinked via UV irradiation using Irgacure 2959 by CIBA ® as the photoinitiating agent. The characterization of the materials was accomplished by: attenuated total reflectance-Fourier transform infrared (ATR-FTIR), swelling capacity determination, evaluation of adhesive capacity (by reaction with aminated substrates) and determination of surface energy by contact angle measurement. Thermal characterization of the adhesive was performed by dynamical mechanical thermal analysis (DMTA) and thermogravimetric analysis (TGA). The morphology of PCL networks was observed using scanning electron microscopy (SEM) both after crosslinking process and following biodegradation in human plasma.The haemocompatibility of the membranes was also evaluated by thrombosis and haemolysis tests.

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A new resorbable monofilament suture

Cited by 112 publications

References 3 publications

In vitro degradation and biocompatibility of poly(DL‐lactide‐ϵ‐caprolactone) nerve guides

In vitro degradation and biocompatibility of poly(DL‐lactide‐ϵ‐caprolactone) nerve guides

The pH dependence of monofilament sutures on hydrolytic degradation

Development of a new photocrosslinkable biodegradable bioadhesive

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