A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

Abstract

a b s t r a c tA simple, sensitive and selective fluorescent chemo dosimeter rhodamine B phenyl hydrazide (RBPH) for Cu 2 þ was proposed. This probe is non fluorescent and colorless but exhibits fluorescent enhancement at 580 nm and displayed color change from colorless to pink for Cu 2 þ in the pH range 1-6. Fluorescence microscope experimental results reveals that this chemo sensor is cell permeable and can be used for fluorescence imaging of Cu 2 þ ions in living cells. This probe can detect Cu 2 þ with good linear relationships from 10 to 100 nM with r ¼0.99971 then limit of detection was found to be 0.015 nM with 7 0.91% RSD at 10 nM concentrations.

Effects of Single and Double Bonds in Linkers on Colorimetric and Fluorescent Sensing Properties of Polyving Akohol Grafting Rhodamine Hydrazides

“…In a standard cuvette, 5 μL of 0.1 mol/L Cu 2+ solution was added to 2 mL of 8.5 mg/mL PVA-MRBH ([Cu 2+ ]=2.5× 10 −4 mol/L) and the fluorescence emission (λex=500 nm) values were measured and plotted as function of time [39]. As shown in Fig.…”

A rhodamine‐triazole fluorescent chemodosimeter for Cu2+ detection and its application in bioimaging

“…For Czarnik's rhodamine hydrazide, Cu 2+ promotes a hydrolytic reaction and results in an enhanced fluorescence emission but the chemical probe was later reported to react with Hg 2+ preferentially . Other Cu 2+ ‐selective rhodamine hydrazide derivatives have also been reported as well as rhodamine hydrazide conjugated with different receptors including isothiocyanate, 4‐(dimethylamino)benzaldehyde, sulfonyl, acyl, phenyl, pyridine, semicarbazide, thiosemicarbazide, vanillic aldehyde, 4‐chloro‐7‐nitro‐2,1,3‐benzoxadiazole, 4‐(diphenylamino)benzaldehyde, 1,4‐(5′‐ethynylsalicyaldehyde)‐ N ‐butyl‐1,8‐naphthalimides and the o ‐pyridylaldehyde group have been synthesized to explore the binding affinity for Cu 2+ . Significantly, 1,2,3‐triazole based on click chemistry plays various roles in metal sensing such as a multidentate motif for metal ions, a linker between a binding site and a reporter or as a part of a conjugated fluorophore .…”

“…benzaldehyde, [30] sulfonyl, [31] acyl, [32] phenyl, [33] pyridine, [34] semicarbazide, [35] thiosemicarbazide, [36] vanillic aldehyde, [37] 4-chloro-7-nitro-2,1,3-benzoxadiazole, [38] 4-(diphenylamino)benzaldehyde, [39] 1,4-(5′-ethynylsalicyaldehyde)-N-butyl-1,8-naphthalimides [40] and the o-pyridylaldehyde [41] group have been synthesized to explore the binding affinity for Cu 2+ . Significantly, 1,2,3-triazole based on click chemistry plays various roles in metal sensing such as a multidentate motif for metal ions, a linker between a binding site and a reporter or as a part of a conjugated fluorophore.…”

A novel mixed matrix membrane of phenolphthalein hydrazide and polysulfone for the detection of copper ions in environmental water samples

“…The results of both these Cu 2+ ion concentration determination-testing methods are presented in Table 1. The obtained results were statistically compared with the Student-t test and variance ratio F-test with fluorimetric and AAS method results [22]. The Student t-test values did not exceed theoretical value sat 95% confidence level suggests that there is no significant difference between the results of proposed and fluorimetric/AAS methods [22].…”