A new root-knot nematode, Meloidogyne moensi n. sp. (Nematoda: Meloidogynidae), parasitizing Robusta coffee from Western Highlands, Vietnam

Abstract

A new root-knot nematode, parasitizing Robusta coffee in Dak Lak Province, Western Highlands of Vietnam, is described as Meloidogyne moensi n. sp. Morphological and molecular analyses demonstrated that this species differs clearly from other previously described root-knot nematodes. Morphologically, the new species is characterized by a swollen body of females with a small posterior protuberance that elongated from ovoid to saccate; perineal patterns with smooth striae, continuous and low dorsal arch; lateral lines marked as a faint space or linear depression at junction of the dorsal and ventral striate; distinct phasmids; perivulval region free of striae; visible and wide tail terminus surrounding by concentric circles of striae; medial lips of females in dumbbell-shaped and slightly raised above lateral lips; female stylet is normally straight with posteriorly sloping stylet knobs; lip region of second stage juvenile (J2) is not annulated; medial lips and labial disc of J2 formed dumbbell shape; lateral lips are large and triangular; tail of J2 is conoid with rounded unstriated tail tip; distinct phasmids and hyaline; dilated rectum. Meloidogyne moensi n. sp. is most similar to M. africana, M. ottersoni by prominent posterior protuberance. Results of molecular analysis of rDNA sequences including the D2-D3 expansion regions of 28S rDNA, COI, and partial COII/16S rRNA of mitochondrial DNA support for the new species status.

Diversity of plant‐parasitic nematodes (PPNs) associated with medicinal plants in Vietnam, Vietnamese PPN checklist and a pictorial key for their identification

“…Later on, Nguyen and Nguyen (2000) provided the first full checklist of PPNs in Vietnam, which included detailed morphological characterizations of 160 species. Since the initial release of this list, numerous new species descriptions and new genus records in Vietnam have been published, including new species of the genera Apratylenchus (Trinh, Waeyenberge, et al, 2009), Geocenamus (Le et al, 2019), Hoplolaimus (Nguyen et al, 2015), Meloidogyne (Le et al, 2019; Trinh et al, 2018), Pratylenchus (Nguyen, Le, et al, 2017) and Radopholus (Nguyen et al, 2003; Trinh et al, 2004; Trinh, Waeyenberge, et al, 2012). Furthermore, the classification of many taxa has also been revised (Chitambar & Subbotin, 2014; Decraemer & Hunt, 2013; Geraert, 2010, 2013; Ghaderi et al, 2016; Subbotin et al, 2021).…”

Suppressiveness of soil amendments with pelleted plant materials on the root-knot nematode Meloidogyne incognita

“…Root-knot nematodes ( Meloidogyne spp.) are included among the most dangerous crop pests due to the severe yield losses caused to a wide range of worldwide crops ( Moens et al, 2009 ; Le et al, 2019 ). The severe restrictions to the use of synthetic nematicides, imposed by their negative environmental effects, have given a strong impulse to the research on more sustainable alternative control strategies ( Tilman et al, 2002 ).…”

Occurrence of endophytic bacteria in Vietnamese Robusta coffee roots and their effects on plant parasitic nematodes

“…and the lesion nematode Pratylenchus spp. (Chapman 2014;Le et al, 2019;Trinh et al, 2009;Villain et al 2018;Wirryadiaputra & Tran, 2008), which are one of the most aggressive and harmful plant-parasitic nematodes attacking coffee plantations (Bell et al, 2018;Bertrand et al 2000). The leaves of trees infected with nematodes become yellow, and show symptoms of rot root or galling.…”