A new sample preparation protocol for SEM and TEM particulate matter analysis

Sinha, Ankur; Ischia, Gloria; Straffelini, Giovanni; Gialanella, Stefano

doi:10.1016/j.ultramic.2021.113365

Cited by 6 publications

(4 citation statements)

References 37 publications

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“…The PM sample preparation followed a protocol that was described by Sinha et al [40]. The PM that was collected on the aluminum foils from the impactor was transferred onto an acetate foil and was coated with a carbon film (through sputtering).…”

Section: Sample Preparation Of Pm For Sem and Tem Analysismentioning

confidence: 99%

Dry Sliding Behavior and Particulate Emissions of a SiC-graphite Composite Friction Material Paired with HVOF-Coated Counterface

et al. 2022

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Dry sliding wear tests and corresponding particulate matter (PM) analysis were conducted on a newly developed SiC-graphite-based composite friction material, paired with two types of HVOF counterface/discs: WC-CoCr and WC-FeCrAlY coatings, with a conventional martensitic stainless steel counterface as a reference. The trials were conducted on a pin-on-disc testing equipment at room temperature and a constant sliding velocity and contact pressure of 7 m/s and 0.5 MPa, respectively. The coefficient of friction (CoF) curves with the uncoated disc exhibited considerable fluctuations. On the other hand, the coated discs featured an increase in the CoF at the beginning of the tests, followed by either a continuous reduction until the end of the testing duration or the attainment of a steady state regime. The pin wear and emissions with both coatings were appreciably lower when compared to the trials with the uncoated disc. The evaluation of the friction layer observed a significant contribution of the counterface for all the pairings. The PM analysis was conducted on the particles that were lying in the range of 10 μm and 2.5 μm on a scanning electron microscope (SEM), and particles from 2.5 μm and 1 μm on transmission electron microscope (TEM), with an emphasis on the particles that were detached from the pin surface and friction layer to explain the wear mechanisms for each pairing. Through this, the need for the proper selection of both friction material and counterface to avoid the emission of harmful compounds in the environment was highlighted.

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Section: Sample Preparation Of Pm For Sem and Tem Analysismentioning

confidence: 99%

Dry Sliding Behavior and Particulate Emissions of a SiC-graphite Composite Friction Material Paired with HVOF-Coated Counterface

et al. 2022

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“…A few drops of the resulting suspension were deposited onto a carbon-coated Cu (300 mesh) TEM grid. For the disc brake wear debris, the sample preparation method was based on the approach described by Sinha et al (2021).…”

Section: Methodsmentioning

confidence: 99%

Electron diffraction characterization of nanocrystalline materials using a Rietveld-based approach. Part II. Application to microstructural analysis

et al. 2022

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This is the second in a two-paper series concerning the quantitative characterization of nanocrystalline materials using an electron-diffraction-based approach, in which a full-pattern fitting Rietveld-based refinement is applied to electron powder diffraction data in transmission electron microscopy (TEM). Part I [Sinha et al. (2022). J. Appl. Cryst. 55, 953–965] established a standard calibration protocol to determine the instrumental effects, with special emphasis on the camera length and the diameter of the selected area apertures. Possible application cases are now considered to demonstrate the capabilities of the approach, including the evaluation of the phase composition of TEM specimens, an operation that reveals new application fields for this powerful materials characterization technique. In this regard, different types of material specimen are examined: nanocrystalline yttrium oxide, silicon, titanium dioxide and debris from disc brake wear, each one featuring specific aspects to be tackled with the proposed methodology. To demonstrate the limits of the proposed approach as concerns the material characteristics, an analysis of a hematite sample obtained from the heat treatment of natural goethite, displaying a relatively coarse crystallite size, is performed and a critical discussion of the results is given.

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“…The midgut was immersed in PBS and diluted into 2.5% glutaraldehyde and fixed overnight at room temperature. The obtained midguts were prepared according to the method of transmission electron microscopy (TEM) sample preparation of Sinha et al (2021). Uranium acetate peroxide dyeing was conducted for 10 min, lead acetate dyeing for 10 min, and a JEM-1230 (Joel, Tokyo, Japan) was used for electron microscopic observation.…”

Section: Transmission Electron Microscope Observationmentioning

confidence: 99%

The Combination Analysis Between Bacillus thuringiensis Sip1Ab Protein and Brush Border Membrane Vesicles in Midgut of Colaphellus bowringi Baly

Cao

Xiao

et al. 2022

Front. Microbiol.

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The secretory insecticidal protein Sip1Ab and crystal protein Cry8Ca from Bacillus thuringiensis (Bt) are widely recognized for their coleopteran insecticidal activities. It is worthwhile to investigate the insecticidal mechanisms of these two proteins against Colaphellus bowringi Baly, which is a serious pest of cruciferous vegetables in China and other Asian countries. To that end, the genes encoding the Sip1Ab and Cry8Ca proteins were amplified from the strain QZL38 genome, then expressed in Escherichia coli, after which bioassays were conducted in C. bowringi larvae. After feeding these two proteins, the histopathological changes in the midguts of C. bowringi larvae were observed using transmission electron microscopy (TEM), and the Brush Border Membrane Vesicle (BBMV) was extracted for competition binding assays. TEM showed that ingestion of Sip1Ab caused a significant reduction in growth of the larvae, disruption of midgut microvilli, and expansion of intercellular spaces. Competition binding assays demonstrated that Sip1Ab bound to C. bowringi BBMV with a high binding affinity. However, a mixture of the two proteins in equal proportions showed no significant difference in insecticidal activity from that of Sip1Ab. These results could provide a molecular basis for the application of Sip1Ab in coleopteran insect control and contribute to the study of the Sip1Ab insecticidal mechanism as well.

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A new sample preparation protocol for SEM and TEM particulate matter analysis

Cited by 6 publications

References 37 publications

Dry Sliding Behavior and Particulate Emissions of a SiC-graphite Composite Friction Material Paired with HVOF-Coated Counterface

Dry Sliding Behavior and Particulate Emissions of a SiC-graphite Composite Friction Material Paired with HVOF-Coated Counterface

Electron diffraction characterization of nanocrystalline materials using a Rietveld-based approach. Part II. Application to microstructural analysis

The Combination Analysis Between Bacillus thuringiensis Sip1Ab Protein and Brush Border Membrane Vesicles in Midgut of Colaphellus bowringi Baly

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