A new saw technique improves preparation of bone sections for light and electron microscopy

Klein, C. P. A. T.; Sauren, Y. M. H. F.; Modderman, W. E.; Waerden, J. P. C. M. van der

doi:10.1002/jab.770050413

Cited by 60 publications

(31 citation statements)

References 9 publications

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“…Thereafter, tissue blocks were dehydrated in an ethanol series and embedded in methyl methacrylate. Longitudinal mid-implant histological sections with a thickness of 10-15 m were made on a modified inner-lock histological diamond saw (Leiden Microtome Cutting System, Leiden, NL) 32 and stained with 0.3% basic fuchsine and 1.0% methylene blue, pH 8.5.…”

Section: Tissue Processingmentioning

confidence: 99%

Healing of gaps around calcium phosphate-coated implants in trabecular bone of the goat

Clemens

Klein

Sakkers

et al. 1997

J. Biomed. Mater. Res.

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Hydroxylapatite coatings are under clinical investigation in orthopaedics and dentistry. Bone formation on apatite coatings in the presence of gaps is important for clinical applications. The importance of the stability of the coating is not known at present. By varying the plasma-spray parameters, and by the addition of fluoride, the crystallinity and stability of calcium phosphates can be changed. It is suggested that bone formation is enhanced by dissolution of the apatite coating. We studied apatite coatings of varying stability with regard to their gap-healing characteristics, and we examined what the maximum gap would be that can be bridged if a coating is applied. Ti-6A1-4V implants coated with 62% crystalline hydroxylapatite, 30% crystalline hydroxylapatite or fluorapatite, or noncoated Ti-6A1-4V were implanted in 16 goats. The implants were surrounded by gaps of 1 or 2 mm, and the follow-up period was 6 weeks. Histological examination and histometry revealed that gaps of 1 mm can be bridged by bone if an apatite coating is applied. However, only a minimal amount of bone contact was seen on the apatite coatings with 2 mm gaps. Uncoated implants demonstrated no bone contact at all. Among the three different coatings there were no differences in gap healing. It can be concluded that in the goat, gaps of 2 or more mm between coated implants and host bone tissue inhibit bone deposition on the coating (p < 0.05), but the stability of the coating does not influence gap-healing characteristics.

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Section: Tissue Processingmentioning

confidence: 99%

Healing of gaps around calcium phosphate-coated implants in trabecular bone of the goat

Clemens

Klein

Sakkers

et al. 1997

J. Biomed. Mater. Res.

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“…One strategy to achieve this goal is to precoat implants with cell adhesion molecules. Fibroblast adhesion to metal implants with and without pretreatment of the implants with various cell adhesion molecules has been well documented [Cannas et al, 1988;Curtis and Forrester, 1984;Dejana et al, 1987;Guy et al, 1993;Hayman et al, 1985;Hughes et al, 1993;Hynes, 1992;Klein et al, 1994;Kornu et al, 1996;Lowenberg et al, 1988;Michaels et al, 1991]. Similarly, a number of studies have revealed how osteoblast adhesion to implants influences osseointegration [Kornu et al, 1996;Puleo et al, 1991;Shah et al, 1999;Sinha et al, 1994;Vrouwenvelder et al, 1993].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of Titanium Alloy and Collagen Type I on Cell Adhesion, Proliferation and Differentiation of Osteoblast-Like Cells

Roehlecke

Witt²,

Kasper³

et al. 2001

Cells Tissues Organs

114

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A number of studies have demonstrated the pivotal role of collagen in modulating cell growth and differentiation. In bone, where the extracellular matrix is composed of approximately 85% type I collagen, cellular interaction with matrix components has been shown to be important in the regulation of the osteoblast phenotype. Preservation or enhancement of normal osteoblast function and appositional bone formation after implant placement represents a strategy that can be useful for the purpose of improving osseointegration. In order to further improve biocompatibility, we combined two known favorable compounds, namely the titanium alloy, Ti6A14V, with type I collagen. We assessed the in vitro behavior of primary osteoblasts grown on both fibrillar collagen-coated and tropocollagen-coated Ti6A14V in comparison with uncoated titanium alloy, using an improved adsorption procedure. As parameters of biocompatibility, a variety of processes, including cell attachment, spreading, cytoskeletal organization, focal contact formation, proliferation and expression of a differentiated phenotype, were investigated. Our results demonstrated for the first time that in comparison to uncoated titanium alloy, collagen-coated alloy enhanced spreading and resulted in a more rapid formation of focal adhesions and their associated stress fibers. Growing on collagen-coated Ti6A14V, osteoblasts had a higher proliferative capacity and the intracellular expression of osteopontin was upregulated compared to uncoated titanium alloy. Type I collagen-coated titanium alloy exhibits favorable effects on the initial adhesion and growth activities of osteoblasts, which is encouraging for its potential use as bone graft material. Moreover, collagen type I may serve as an excellent biocompatible carrier for osteotropic factors such as cell adhesion molecules (e.g. fibronectin) or bone-specific growth factors.

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“…The vertical section method was applied as has been previously described [20][21][22] and unbiased estimates of the parameters were obtained. The sections were spaced 425 µm apart and were cut in one step on a newly developed microtome (KDG 95 microtome; Ignition, Bilthoven, The Netherlands) 23 Diagram of the implant device showing a hollow anchorage screw with a spring (1) and a piston (2) on which the test implant (3) is mounted. An ultra-highmolecular-weight-polyethylene plug (PE) (4) projects above the surface of the articular cartilage.…”

Section: Methodsmentioning

confidence: 99%

The influence of crystallinity of the hydroxyapatite coating on the fixation of implants

Overgaard

Bromose

Lind

et al. 1999

The Journal of Bone and Joint Surgery. British volume

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W e inserted two hydroxyapatite (HA)-coated implants with crystallinities of either 50%(HA-50%) or 75% (HA-75%) bilaterally into the medial femoral condyles of the knees of 16 dogs. The implants were allocated to two groups with implantation periods of 16 and 32 weeks. They were weight-bearing and subjected to controlled micromovement of 250 µm during each gait cycle. After 16 weeks, mechanical fixation of the HA-50% implants was increased threefold as compared with the HA-75% implants. After 32 weeks there was no difference between HA-50% and HA-75%. Fixation of HA-75% increased from 16 to 32 weeks whereas that of HA-50% was unchanged. HA-50% implants had 100% more bone ingrowth than HA-75% implants after 16 weeks. More HA coating was removed on HA-50% implants compared with HA-75% implants after both 16 and 32 weeks. No further loss of the HA coating was shown from 16 to 32 weeks.Our study suggests that the crystallinity of the HA coating is an important factor in its bioactivity and resorption during weight-bearing conditions. Our findings suggest two phases of coating resorption, an initial rapid loss, followed by a slow loss. Resorbed HA coating was partly replaced by bone ingrowth, suggesting that implant fixation will be durable. J Bone Joint Surg [Br] 1999;81-B:725-31.

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A new saw technique improves preparation of bone sections for light and electron microscopy

Cited by 60 publications

References 9 publications

Healing of gaps around calcium phosphate-coated implants in trabecular bone of the goat

Healing of gaps around calcium phosphate-coated implants in trabecular bone of the goat

Synergistic Effect of Titanium Alloy and Collagen Type I on Cell Adhesion, Proliferation and Differentiation of Osteoblast-Like Cells

The influence of crystallinity of the hydroxyapatite coating on the fixation of implants

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