A new selective blood agar medium for Streptococcus pyogenes and other haemolytic streptococci

Lowbury, E. J. L.; Kidson, A.; Lilly, H. A.

doi:10.1136/jcp.17.3.231

Cited by 18 publications

(9 citation statements)

References 8 publications

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“…All samples were inoculated onto three isolation media: 1) Esculin blood agar consisting of peptone (Difco) 11.66 g, agar 12.0 g, meat extract (Fleischextrakt trocken, Merck) 4.0 g, Na2HP04, 2H,O 1.5 g, NaCl 5.0 g, yeast extract (Difco) 3.0 g, esculin (Merck) 1.0 g, tryptone (Difco) 8.3 g, glucose (Merck) 0.44 g and distilled water 1000 ml (pH 7.4), supplied with 5% sterile bovine stabilized blood. 2) Selective streptococcus medium (20,26), which also allows growth of yeasts and algae, consisting of esculin blood agar supplied with neomycin 0.425 mg / 100 ml, polymyxin B sulphate (Sigma) 0.22 mg/100 ml, and sodium fusidate (Leo) 0.05 mg/100 ml. 3)…”

Section: Materials a N D Methodsmentioning

confidence: 99%

Mycotic and algal bovine mastitis in Denmark

Aalbæk

Stenderup

Jensen

et al. 1994

APMIS

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A one‐year examination of mammary secretions (n = 2,896) from Danish cattle with clinical or subclinical mastitis revealed 45 strains of fungi and algae. The strains originated from 44 mammary secretions of 42 cows in 40 herds. The following species of fungi were identified: Candida catenulata (n = 2), Candida kefyr (n = 6), Candida krusei (n=17), Candida rugosa (n = 6), Candida tropicalis (n = 3), Candida valida (n=1), Geotrichum capitatum (n = 5). The algal species Prototheca zopfii was demonstrated in five samples.

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Section: Materials a N D Methodsmentioning

confidence: 99%

Mycotic and algal bovine mastitis in Denmark

Aalbæk

Stenderup

Jensen

et al. 1994

APMIS

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“…Wound swabs were cultured on blood agar, electrolyte-deficient agar (Sandys, 1960) and MacConkey's agar. Sputa were cultured on PNF blood-agar medium (Lowbury, Kidson and Lilly, 1964), chocolate agar, and electrolyte-deficient agar, after homogenisation with N-acetyl cysteine. Sensitivity tests were performed by the flood-plate method on Diagnostic Sensitivity Test (DST, Oxoid) agar (Iso-Sensitest, Oxoid, since April 1976) enriched with 10% lysed horse blood.…”

Section: Methodsmentioning

confidence: 99%

Human Infections with Thymine-Requiring Bacteria

Maskell

Okubadejo

Payne³

et al. 1978

Journal of Medical Microbiology

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“…Gram films were made and the sputum was cultured by the method of Rawlins (1968) using pancreatintrypsin to liquefy and homogenize the sputum which was cultured in dilutions of 1 in 2 and 1 in 10-4 on chocolate agar in 10% carbon dioxide, on blood agar (Southern Group) aerobically, on polymyxin-neomycin-fusidate (PNF) plates (Lowbury et al, 1964) anaerobically (Gaspak system), and on MacConkey plates. The organisms were identified using the methods and tables of Cowan and Steel (1974).…”

Section: Laboratory Techniquesmentioning

confidence: 99%

Pneumonia caused by coliforms and Pseudomonas aeruginosa.

Noone¹,

Rogers²

1976

J Clin Pathol

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NW3soNopsis The diagnosis and treatment of 20 hospital patients seen in the past year with proven pneumonia caused by coliforms and Pseudomonas aeruginosa are discussed. Predisposing factors and methods for improving laboratory and clinical diagnosis are analysed, the main problem being to discriminate between genuine pneumonia caused by these organisms and mere contamination of sputum samples resulting from colonization of the upper respiratory tract following broad-spectrum chemotherapy.Overall initial chemotherapy with gentamicin cured 75% (15 out of 20) of the patients in spite of unfavourable underlying pathology. Where gentamicin was given in adequate dosage, which in practice meant that dose which produced peak serum concentrations of 8 ,ug/ml or more, the cure rate was 91% (11 out of 12). In those patients achieving (measured) peak serum concentrations of less than 8 ,ug/ml the cure rate was only 33% (4 out of 12). These figures include four patients who failed to respond to doses of gentamicin producing peak concentrations of 5-0-6-0 ,ug/ml in each case. These patients responded promptly to higher doses (or accumulation), producing peak serum concentrations of 8 ,ug/ml or more and were then cured within three to five days.Toxicity from gentamicin was not observed in any patient. These results indicate that it is necessary to monitor gentamicin therapy by laboratory assay to ensure adequate dosage and that peak serum concentrations of 8 ,ug/ml or more are significantly correlated with successful treatment ofpneumonia caused by coliforms and Ps. aeruginosa.

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A new selective blood agar medium for Streptococcus pyogenes and other haemolytic streptococci

Abstract: Haemolytic streptococci of groups A, C, D, G, and others were isolated by aerobic culture from burns in a consecutive series of 1,277 swabs more often on P.N.F. medium than on blood 4% agar.

Cited by 18 publications

References 8 publications

Mycotic and algal bovine mastitis in Denmark

Mycotic and algal bovine mastitis in Denmark

Human Infections with Thymine-Requiring Bacteria

Pneumonia caused by coliforms and Pseudomonas aeruginosa.

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