A new set of microsatellite markers for the genetic characterization of Ceratocystis fimbriata, an economically important plant pathogen

Abstract

Ceratocystis fimbriata is a fungal pathogen which attacks several economically important plants, but occurs in host-associated, morphologically indistinguishable forms. In Brazil, this fungus seriously attacks mango trees (Mangifera indica), causing severe loss of yield. This work aimed to develop and characterize a novel set of microsatellite markers for this important pathogen, providing researchers with new molecular tools for the characterization of isolates. Twenty polymorphic primer pairs were designed from a microsatellite-enriched library. We tested the usefulness of these markers through genotyping thirteen isolates of the fungus. On average, 6.65 alleles per locus were detected, revealing the ability of this set of markers to characterize C. fimbriata isolates associated to mango and to other plant species.

Development of Microsatellite Markers and Analysis of Genetic Diversity and Population Structure of Colletotrichum gloeosporioides from Ethiopia

“…However, when polymorphic loci are available, they can be very useful for genome mapping, and genetic diversity and population genetic studies [ 60 ]. Accordingly, SSRs have been used for the study of the genetic diversity of various plant pathogenic fungi including Ascochyta rabiei [ 61 ], Ceratocystis fimbriata [ 62 ], Macrophomina phaseolina [ 63 ], Puccinia graminis and P . triticina [ 6 , 64 , 65 ], Sclerotinia subarctica and S .…”