Lunar dust is regarded as the most crucial environmental problem on the Moon, and related research has crucially important scientific and technological interests. Here, we first reported the in situ measurements of lunar dust at the Chang'E‐3 landing site in the northern Mare Imbrium using temperature‐controlled sticky quartz crystal microbalance. The results showed that a total deposition mass at a height of 190 cm above the lunar surface during 12 lunar daytimes in the northern Mare Imbrium was about 0.0065 mg/cm2, corresponding to an annual deposition rate of ~21.4 μg/cm2, which is comparable with that of Apollo's result to some extent. The present researches are strategically important for future human and robotic lunar expeditions, and can provide a valuable reference for the design of dust protection for onboard payloads long‐term exposure to the lunar environment.