A new solid phase extraction clean‐up method for the determination of 12 type A and B trichothecenes in cereals and cereal‐based food by LC‐MS/MS

Klötzel, Marianna; Lauber, U.; Humpf, Hans‐Ulrich

doi:10.1002/mnfr.200500234

Cited by 89 publications

(66 citation statements)

References 28 publications

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“…Many of these are designed for determining a certain group or an otherwise restricted number of compounds in cereals (Razzazi-Fazeli et al 2002Berthiller et al 2005;Biselli and Hummert 2005;Klötzel et al 2006;Ren et al 2007;Lantazzio et al 2007). Fewer analytes permit a more selective procedure and sample preparation, and different, specific cleanup columns like Mycosep 227®, Mycosep 226 Aflazon+®, Vicam Myco6in1®) have been utilized in these methods.…”

Section: Introductionmentioning

confidence: 98%

“…Several different types of LC-MS multi-mycotoxin methods for food and feed matrices have been published recently (e.g., Razzazi-Fazeli et al 2002Berthiller et al 2005;Biselli and Hummert 2005;Klötzel et al 2006;Ren et al 2007;Lantazzio et al 2007; Rundberget and Wilkins 2002;Kokkonen et al 2005;Sulyok et al 2006Sulyok et al , 2007aSpanjer et al 2008). Many of these are designed for determining a certain group or an otherwise restricted number of compounds in cereals (Razzazi-Fazeli et al 2002Berthiller et al 2005;Biselli and Hummert 2005;Klötzel et al 2006;Ren et al 2007;Lantazzio et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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A Multi-compound LC-MS/MS Method for the Screening of Mycotoxins in Grains

Kokkonen

Jestoi

2008

Food Anal. Methods

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A method development and its validation are described for determining 31 selected Aspergillus, Fusarium, Penicillium, and Claviceps mycotoxins in small grain cereals (wheat, barley, oats). The method comprises an automated solvent extraction step followed by filtering, concentrating, and the analysis of the crude extract with liquid chromatographic-tandem mass spectrometric determination. The analytes included trichothecenes, zearalenone, fumonisins, moniliformin, enniatins, beauvericin, antibiotic Y, aflatoxins, ochratoxin A, mycophenolic acid, penicillic acid, and ergot alkaloids. These were separated in two consecutive chromatographic runs, both involving negative and positive electrospray ionization modes of mass spectrometry. The validation showed that the method performance was good for (semi-)quantitative work, limits of quantification varying between 1 and 1,250 μg kg −1 , recoveries mostly between 51% and 122%, and repeatability being from 2% to 26% within day and from 14% to 28% between days (as relative standard deviation). A distinctive suppressive matrix effect, which depended on the analyte and the matrix, was observed but could be compensated for by using matrix-assisted standards. The developed multimycotoxin method permits simultaneous, simple, and rapid determination of several co-existing toxins and is ideal for, e.g., screening-type work or at concentration levels relevant in animal welfare.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

A Multi-compound LC-MS/MS Method for the Screening of Mycotoxins in Grains

Kokkonen

Jestoi

2008

Food Anal. Methods

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“…Briefly, after homogenization and addition of the IS, the samples were extracted with an ACN/water mixture. The extract was filtered and the clean up was performed using a new type of Bond Elut TM column as recently published by Klötzel et al [17]. After a further concentration step, the samples were analyzed by HPLC-MS/MS.…”

Section: Resultsmentioning

confidence: 99%

“…Sample cleanup was performed using the method of Klötzel et al [17]. Briefly, 4 mL of the extract was passed through a Varian Bond Elut Mycotoxin TM cartridge.…”

Section: Sample Preparationmentioning

confidence: 99%

Stable isotope dilution analysis of the Fusarium mycotoxins deoxynivalenol and 3‐acetyldeoxynivalenol

Bretz

Beyer

Cramer

et al. 2006

Molecular Nutrition Food Res

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Trichothecenes are secondary metabolites produced by several fungi of the Fusarium genus during their growth period. They inhibit protein biosynthesis in eukaryotic cells resulting in numerous toxic effects such as diarrhea, vomiting, and gastro-intestinal inflammation. Considering its occurrence in food and feedstuff, deoxynivalenol (DON) is one of the most important trichothecenes. We report the synthesis of stable isotope labeled 15-d(1)-deoxynivalenol (15-d(1)-DON) from its natural precursor 3-acetyldeoxynivalenol (3-AcDON) as starting material. Furthermore, a method for the analysis of DON and 3-AcDON using HPLC-MS/MS with stable isotope labeled 15-d(1)-DON and 3-d(3)-AcDON as internal standards has been developed. In total, 18 cereal product samples were analyzed with contamination levels ranging from 10-301 microg/kg for DON and 5-14 microg/kg for 3-AcDON. This is the first report of an isotope dilution MS method for the analysis of type B-trichothecenes.

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“…Most food commodities contaminated by T-2 also contain the hydroxylated HT-2 metabolite (Klötzel et al, 2006;Molinelli et al, 2008;Trebstein et al, 2008). Thus, we used the CCL2 and SGG marker transcripts to determine how the bioassay results were influenced by the simultaneous exposure of MCF7 cells to both T2 and HT-2.…”

Section: Additivity and Specificity Of The Transcriptomic-based Bioassaymentioning

confidence: 99%

Transcriptomic-based bioassays for the detection of type A trichothecenes

Lancová

Bowens

Stroka

et al. 2009

WMJ

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The type A trichothecenes T-2 toxin (T-2) and HT-2 toxin (HT-2) are hazardous Fusarium products that contaminate many field crops growing in cold to temperate regions across the world. Toxicity studies in laboratory and farm animals have been used to derive a temporary tolerable daily intake (t-TDI) for the sum of T-2 and HT-2 of no more than 60 ng/kg body weight. To protect the consumers, it is now necessary to screen a large number of food samples for the presence of these poisonous fungal metabolites. Towards that goal, we discovered that the transcriptional apparatus of a human carcinoma cell line (MCF7) provides a sensitive biological sensor of type A trichothecenes. In fact, exposure of this easy-to-culture cell line to T-2 or HT-2 results in the regulation of >2,000 different transcripts with expression changes ranging from >5,000-fold gene inductions to >40-fold gene repressions. These transcriptional responses have been exploited to develop practical microchip and reverse transcription-polymerase chain reaction (RT-PCR) assays for the detection of type A trichothecenes at parts per billion levels.

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A new solid phase extraction clean‐up method for the determination of 12 type A and B trichothecenes in cereals and cereal‐based food by LC‐MS/MS

Cited by 89 publications

References 28 publications

A Multi-compound LC-MS/MS Method for the Screening of Mycotoxins in Grains

A Multi-compound LC-MS/MS Method for the Screening of Mycotoxins in Grains

Stable isotope dilution analysis of the Fusarium mycotoxins deoxynivalenol and 3‐acetyldeoxynivalenol

Transcriptomic-based bioassays for the detection of type A trichothecenes

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