A new species of Arthrographis (Eremomycetaceae, Dothideomycetes), from the soil in Guizhou, China

Abstract

During a survey of keratinolytic fungi in China, a new species, Arthrographis multiformispora was isolated from soil samples. Morphologically, A. multiformispora differs from other species in the genus by the presence of globose or subglobose chlamydospores and cylindrical arthroconidia. Phylogenetically, our four strains were clustered together with high support values and separated from other clades. We provided a description, illustrations, and phylogenetic tree for the new species.

Multiverruca sinensis gen. nov., sp. nov., a thermotolerant fungus isolated from soil in China

“…The total DNA was extracted using the BioTeke Fungus Genomic DNA Extraction kit (DP2032, BioTeke), following the manufacturer’s instruction. The total DNA was used for the amplification and sequencing based on two fragments: ribosomal ITS (primers ITS1: 5′-TCCGTAGGTGAACCTGCGG-3′/ITS4: 5′-TCCTCCGCTTATTGATATGC-3′) [19], and the 28S rRNA gene (nrLSU; primers LR0R: 5′-GTACCCGCTGAACTTAAGC-3′/LR5 : 5′-ATCCTGAGGGAAACTTC-3′) [20]. The PCR thermal cycle programme for ITS and LSU amplification was as follows [2]: initial denaturation of 94 °C for 3 mins, followed by 40 cycles of denaturation at 95 °C for 30 s, annealing at 55 °C for 50 s, elongation at 72 °C for 1 min.…”

“…Then, the alignments were concatenated and phylogenetic analysis was performed. Maximum-likelihood (ML) and Bayesian inference (BI) approaches were used for the phylogenetic analyses using PhyloSuite v1.16 [19,22]. For ML analysis, the best-fit model (Table 2) of substitution for each locus was estimated using iq-tree's ModelFinder function [23,24] based on the corrected Akaike Information Criterion (AICc).…”

Morphological characteristics and phylogenetic evidence reveal two new species of Acremonium (Hypocreales, Sordariomycetes)

“…We washed the chicken feathers, sterilized them in an autoclave for 30 minutes at 121 °C, and dried them in an oven at 50 °C. The sterile and dried chicken feathers were mixed with soil samples and then wet with sterile distilled water and cultured at darkroom temperature for 1 month (Li et al 2022).…”

“…Bayesian inference (BI) and maximum likelihood (ML) methods were used in the analysis. For BI analysis was conducted with MrBayes v3.2 (Ronquist et al 2012) and Markov chain Monte Carlo (MCMC) simulations; ML analysis was performed using IQ-TREE v1.6.11 (Nguyen et al 2015), as outlined in Li et al (2022). All analyses were performed in PhyloSuite V1.16 (Zhang et al 2020b).…”