As a result of systematic study of the Neotropical genus Aspidosperma (Apocynaceae), we reestablish the species A. centrale and A. duckei, and report A. steinbachii and A. tambopatense as new records for Brazil. We provide taxonomic descriptions of these species along with plates, distribution maps, and information on their conservation status, habitat and phenology. We also provide an identification key for all the species of Aspidosperma from the Brazilian Amazon.