A new species of Clinostomum Leidy, 1856 based on molecular and morphological analysis of metacercariae from African siluriform fishes

Caffara, Monica; Locke, Sean A.; Echi, Paul Chinedu; Halajian, Ali; Luus-Powell, WJ; Benini, Deborah; Tedesco, Perla; Fioravanti, Marialetizia

doi:10.1007/s00436-019-06586-2

Cited by 18 publications

(13 citation statements)

References 25 publications

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“…These metacercariae are zoonotic in nature that may cause Laryngo-pharyngitis disease in humans who eat improperly cooked fish, and may lead to death due to asphyxia (Wang et al, 2017). The digenetic trematodes Clinostomidae, including both vertebrates and invertebrates hosts, possess complicated life cycle that have recently stimulated reconsideration depending on both morphological and molecular analysis (Caffara et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Comparative morpho-molecular identification of Clinostomum phalacrocoracis and Clinostomum complanatum metacercaria coinfecting Nile tilapia in Egypt

Salem¹,

Maogood²,

Abdelsalam³

et al. 2021

Egypt. J. of Aquatic Biolo. and Fish.

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Section: Introductionmentioning

confidence: 99%

Comparative morpho-molecular identification of Clinostomum phalacrocoracis and Clinostomum complanatum metacercaria coinfecting Nile tilapia in Egypt

Salem¹,

Maogood²,

Abdelsalam³

et al. 2021

Egypt. J. of Aquatic Biolo. and Fish.

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“…This approach has now been applied to Clinostomum species; C. complanatum (Caffara et al, 2011;Dzikowski et al, 2004); C. phalacrocoracis (Caffara et al, 2014). Moreover, Caffara et al (2020) who described a new species of clinostomum; Clinostomum ukolii n. sp. metacercariae based on molecular and morphological analysis from African siluriform fishes.…”

Section: Introductionmentioning

confidence: 99%

First genetic confirmation of Clinostomidae metacercariae infection in Oreochromis niloticus in Egypt

Mahdy

Abdelsalam

Maogood

et al. 2021

Aquaculture Research

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Clinostomiasis is a zoonotic disease of freshwater fish caused by digenetic trematodes in the genus Clinostomum. This parasite causes 'Laryngopharyngitis' disease in people and may result in human fatalities from asphyxiation when consumed improperly cooked Clinostomum complanatum-encysted metacercariae (EMC) infected fish. This study provides genetic confirmation, phylogenetic analysis and histopathological evaluation of Clinostomidae EMC recovered from infected wild-caught and farmed Nile tilapia (Oreochromis niloticus) in Egypt. Recovered parasites were identified as Clinostomum complanatum, C. phalacrocoracis and Euclinostomum heterostomum using macroscopic and microscopic examination. PCR amplification and sequencing of rDNA-ITS2 gene regions of C. complanatum; C. phalacrocoracis and mtDNA CO1 gene regions of E. heterostomum were carried out. Nucleotide sequences of C. complanatum, C. phalacrocoracis and E. heterostomum were registered in GenBank with accession numbers MT133890.1, MT158303.1 and MW193532.1, respectively. Histopathological findings in buccal cavities and kidney tissues of infected O. niloticus were described.

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“…Morphological analysis is commonly used to identify fish species, and the detection of different morphological parameters and their statistical relationships are necessary for the study of fish biology and taxonomy (Caffara et al., 2020; Laskar, Kumar, Kundu, Tyagi, & Chandra, 2018). The four Megalobrama species have been described and are still distinguished, on the basis of their morphological traits.…”

Section: Introductionmentioning

confidence: 99%

“…However, the validity of this species remains controversial due to no further samples were recorded since then. Therefore, it is generally recognized that there are four species in the genus Megalobrama, and their valid scientific names were M. amblycephala, M. skolkovii, M. hoffmanni and M. pellegrini. Morphological analysis is commonly used to identify fish species, and the detection of different morphological parameters and their statistical relationships are necessary for the study of fish biology and taxonomy (Caffara et al, 2020;Laskar, Kumar, Kundu, Tyagi, & Chandra, 2018). The four Megalobrama species have been described and are still distinguished, on the basis of their morphological traits.…”

mentioning

confidence: 99%

Morphological variation among the fourMegalobramaspecies inferred by X‐ray photography

et al. 2020

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Morphological traits measurement still plays a key role in description, identification, classification and phylogenetics of species. However, the traditional morphological measurement method is inefficient and often destructive to the studied organism. In this study, we propose and test an X‐ray‐based technique for non‐destructive morphological studies of fish. We use this approach to attempt to discriminate four species from the genus Megalobrama on the basis of their morphometric and meristic traits. The results showed that there were significant differences (p < 0.05) in the morphological traits among the four species, and the meristic traits were more stable than the morphometric traits. The result of principal component analysis indicated that morphometric characters of Megalobrama hoffmanni were significantly different from the other three species. Megalobrama pellegrini was used as a model to compare and evaluate the accuracy of the two methods (X‐ray and traditional manual), and the results revealed that except for the undifferentiated traits, most of the morphometric traits detected by the traditional manual method were significantly higher than that detected by the X‐ray photography. Also, many standard deviation values produced by the X‐ray photographic method were smaller. Therefore, X‐ray photography is a suitable method for non‐destructive and accurate detection of morphological parameters in the genus Megalobrama.

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A new species of Clinostomum Leidy, 1856 based on molecular and morphological analysis of metacercariae from African siluriform fishes

Cited by 18 publications

References 25 publications

Comparative morpho-molecular identification of Clinostomum phalacrocoracis and Clinostomum complanatum metacercaria coinfecting Nile tilapia in Egypt

Comparative morpho-molecular identification of Clinostomum phalacrocoracis and Clinostomum complanatum metacercaria coinfecting Nile tilapia in Egypt

First genetic confirmation of Clinostomidae metacercariae infection in Oreochromis niloticus in Egypt

Morphological variation among the fourMegalobramaspecies inferred by X‐ray photography

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