Assam is the second largest state of NorthEast India and treasure trove of Indian orchids. Dibrugarh District is in the located in the eastern part of the state with rich native orchid species. This report is the outcome of our intensive field studies carried out during 2006-2011. We have chosen opportunistic method to cover all representative areas of different habitat. We have surveyed the area covering all the seasons of the year. For microhabitat structure randomly selected quadrat of 5mx5m were laid in the field and noted down all the features. In this study a total of 114 Orchid species under 54 genera have been recorded from the district. The study revealed 29 species as terrestrial and the rest 85 species are epiphytic. Among the terrestrial genus Zeuxine is found as ecologically and taxonomically co mplex group of Orch idaceae. During this field study, 6 species of the genus Zeuxine were recorded viz. Z. clandestine; Z. glandulosa; Z. goodyeroides; Z. longilabris; Z. nervosa and Z. Strateumatica. The present study envisages the threat and status of these species with conservation recommendation. Moreover, this is the first attempt to establish the taxonomic identificat ion to workout currently accepted botanical names of the genus with its ecological status, voucher specimen numbers, habitat, phenology and distribution in this district.