A new species of Talaromyces (Trichocomaceae) from the Xisha Islands, Hainan, China

Abstract

A novel Talaromyces species was isolated from a soil sample of the Xisha Islands, Hainan, China. The culture characterization on four standardized media, microscopic observations and phylogenetic analyses based ITS, BenA and CaM regions indicate that it is new to science and named as Talaromyces xishaensis sp. nov. The new species belongs to section Talaromyces, and is related to T. cnidii, T. flavovirens and T. siamensis in the phylogeny inferred from BenA and CaM genes. It is distinguished from the latter three species by colony morphology, more metulae per verticil, subglobose to ellipsoidal conidia, and slow growth rate on CYA at 37 °C.

New Species of Talaromyces (Fungi) Isolated from Soil in Southwestern China

“…Soil samples for floristic studies of fungi were collected from Chengkou, Wushan, and Wuxi counties in Chongqing and Wanyuan City in Sichuan. Although Talaromyces is a widespread genus and distributed in more than 27 provinces, cities, or regions of China [ 14 ], it has never been reported from the above areas.…”

“…Four standard growth media were used: Czapek yeast autolysate agar (CYA, yeast extract Oxoid, Hampshire, UK), malt extract agar (MEA, Amresco, Solon, OH, USA), yeast extract agar (YES) and potato dextrose agar (PDA). The methods for inoculation, incubation, microscopic examinations, and digital recordings were following our previous studies [ 14 , 15 , 16 ].…”

“…DNA was extracted from the cultures grown on PDA for 7 days using the Plant Genomic DNA Kit (DP305, TIANGEN Biotech, Beijing, China). Polymerase chain reaction (PCR) amplifications of the internal transcribed spacer (ITS), beta-tubulin (BenA), calmodulin (CaM) and RNA polymerase II second largest subunit (RPB2) gene regions were conducted with routine methods [ 14 , 15 , 16 ]. The products were purified and subject to sequencing on an ABI 3730 DNA Sequencer (Applied Biosystems, Bedford, MA, USA).…”

Identification of sustainable trypsin active‐site inhibitors from Nigrospora sphaerica strain AVA‐1

“…After the incubation period, the mycelial tips of each colony were transferred to fresh PDA plates for the isolation of pure strains. The fungal strains were identified based on the microscopic and culture characteristic methods through standard taxonomic manuals of identification [23]. The colonies which were unable to sporulate were considered sterile forms.…”

Discovery and Extrolite Production of Three New Species of Talaromyces Belonging to Sections Helici and Purpurei from Freshwater in Korea

“…On the basis of multigene phylogeny, morphology, and physiology, Yilmaz et al [ 3 ] placed 88 accepted species in seven well-defined sections, namely, Bacillispori , Helici , Islandici , Purpurei , Subinflati , Talaromyces , and Trachyspermi . However, the lists are rapidly increasing with many new Talaromyces species recently described from all over the world and added to sections Helici , Islandici , Purpurei , Subinflati , Talaromyces , and Trachyspermi [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ]. To date, 171 species have been reported in the genus Talaromyces [ 27 ], of which only three species: Talaromyces angelicae , Talaromyces cnidii , and Talaromyces halophytorum were reported from Korea [ 28 , 29 ].…”