The present study is based on oribatid mite material collected in Taiwan in 2017–2019. A list of identified taxa, including 141 species/subspecies from 88 genera and 47 families is provided; of these, 36 species/subspecies, 13 genera and five families are recorded for the first time in the Taiwan fauna, and eight species and one genus are recorded for the first time in the Oriental region. Two new species are described. Tyrphonothrus nivnu n. sp. (description based on adult) is similar to Tyrphonothrus altissimus (Piffl, 1971), T. cajamarcensis (Hammer, 1961) and T. wallworki (Starý and Block, 1995) in general morphological traits (notogastral ridges developed; tridactylous legs; notogastral setae e2, h1, h2, and p2 long, setiform, with attenuate tip, other setae of medium length, setiform; high number of genital setae; absence of notogastral ornamentation; and comparatively large body size), but differs from these listed species by the barbed notogastral setae and comparatively long notogastral setae c1, c2, c3, and e1. Jacotella puyuma n. sp. (description based on adult and tritonymph) is similar to Jacotella neonominata Subías, 2004 in general morphological traits (centrodorsal notogastral part with two longitudinal ridges, posteriorly fused, each ridge with two lateral transverse branches; absence of notogastral ornamentation; notogastral seta h1 distinctly longer than other notogastral setae), but differs from the latter by the narrowly elongate bothridial head, flagellate notogastral setae h1 and p1, the length of notogastral setae, and the absence of additional longitudinal ridges connected lateral branches of median ridges (in adult); and by the long gastronotic seta h1 similar in length to bothridial seta, seta h2 similar to h3 and lp and seta dm clearly longer than da on larval exuvium (in tritonymph).