A New Species of the GenusOrobdella(Hirudinida: Arhynchobdellida: Gastrostomobdellidae) from Kumamoto, Japan, and a Redescription ofO. whitmaniwith the Designation of the Lectotype

Abstract

Orobdella whitmani has three syntype localities, Mt. Kinkazan, Gifu Pref., Mt. Kodaijiyama, Kyoto Pref. and Mt. Kimposan, Kumamoto Pref. The specimens from Mt. Kimposan can be distinguished from those from Mt. Kinkazan and Mt. Kodaijiyama by an annulation of somite XXVI, and possession of a tubular gastroporal duct. Specimens from Mt. Kimposan are described as representing a new species, Orobdella esulcata. Orobdella whitmani is redescribed with designation of a specimen from Mt. Kinkazan deposited at The University Museum, The University of Tokyo, as the lectotype.

Four new species of the genus Orobdella from Shikoku and Awajishima island, Japan (Hirudinida, Arhynchobdellida, Orobdellidae)

“…According to taxonomic studies (Nakano 2010, 2012b, 2016, Nakano and Gongalsky 2014, Nakano and Lai 2012, Nakano and Seo 2014, the new species is distinguished from the eight quadrannulate species (i.e. O. esulcata Nakano, 2010, O. kawakatsuorum Richardson, 1975, O. ketagalan Nakano & Lai, 2012, O. koikei Nakano, 2012b, O. masaakikuroiwai, O. naraharaetmagarum, O. tsushimensis Nakano, 2011a by the following combination of characteristics (Table 2): body length less than or reaching ca.…”

“…O. esulcata Nakano, 2010, O. kawakatsuorum Richardson, 1975, O. ketagalan Nakano & Lai, 2012, O. koikei Nakano, 2012b, O. masaakikuroiwai, O. naraharaetmagarum, O. tsushimensis Nakano, 2011a by the following combination of characteristics (Table 2): body length less than or reaching ca. 5 cm, IV uniannulate, XXV quadrannulate, gonopores separated by 1/2 + 4 [+ (< 1/2)] annuli, gastroporal duct tubular, epididymides in XX to XXI and atrial cornua small ovate.…”

A New Quadrannulate Species of <i>Orobdella</i> (Hirudinida: Arhynchobdellida: Orobdellidae) from Pingtung, Taiwan

“…Orobdella shimadae and O. dolichopharynx, which are respectively indigenous to Okinawajima and Amamioshima islands, possess a rudimentary gastroporal duct, and they completely lack an atrial cornu (Nakano 2011a (Nakano 2010(Nakano , 2012b(Nakano , 2016b(Nakano , 2017b (Nakano 2011a(Nakano , 2012bNakano and Lai 2012). The pharyngeal elongation and degeneration of the gastroporal duct within Orobdella species may correlate with each other.…”

“…Orobdella meisai belonged to a well-supported clade (BS=100%, PP=1.0) containing the other eight species inhabiting the western part of Honshu to Taiwan. The clade was divided into two subclades: a well-supported lineage (BS=99%, PP=1.0) consisted of four species, i.e., O. esulcata Nakano, 2010, O. naraharaetmagarum Nakano, 2016, O. kanaekoikeae Nakano, 2017and O. brachyepididymis Nakano, 2016, inhabiting the western part of Honshu, Shikoku and Kyushu islands of Japan; another lineage was not fully supported in the ML tree (BS=49%, PP=0.92), comprising three species, i.e., O. mononoke , O. shimadae Nakano, 2011and O. dolichopharynx Nakano, 2011 The ND1 sequences (LC314421 and LC314426) obtained from the holotype and paratype of O. meisai exhibit unique genetic variations compared with those of the other known Orobdella leeches. The ND1 sequences of O. meisai lack 97th-111th and 249th-260th bp positions in those of the other congeners.…”

“…According to taxonomic studies (Nakano 2010(Nakano , 2011b(Nakano , 2012aNakano and Lai 2012;Nakano 2014;Nakano and Seo 2014;Nakano 2016aNakano , b, 2017b, the new species is distinguishable from the ten quadrannulate species (O. brachyepididymis, O. esulcata, O. kanaekoikeae, O. kawakatsuorum Richardson, 1975, O. ketagalan, O. koikei Nakano, 2012, O. masaakikuroiwai Nakano, 2014, O. naraharaetmagarum, O. tsushimensis, and O. whitmani Oka, 1895 by the following combination of characters (Table 2): body length, annulation of somites IV and XXV, number of annuli between gonopores, pharynx length, gastroporal duct, epididymidal lengths and atrial cornua. Orobdella meisai can clearly differ from these known quadrannulate species in having 5+1/4 annuli between the gonopores, the pharynx reaching to the posterior part of somite XV, the rudimentary tubular gastroporal duct, and in lacking atrial cornua in the male median reproductive system.…”