A new specific reference gene based on growth hormone gene (GH1) used for detection and relative quantification of Aquadvantage® GM salmon ( Salmo salar L.) in food products

Hafsa, Ahmed Ben; Nabi, Nesrine; Zellama, Mohamed Salem; Saïd, Khaled; Chaouachi, Maher

doi:10.1016/j.foodchem.2015.06.064

Cited by 13 publications

(9 citation statements)

References 27 publications

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“…The first genetically modified animal (AquAdvantage salmon) is on the verge of being approved for human consumption in different countries [ 249 ]. New methods are being developed to detect the genetically modified salmon in food products [ 250 , 251 ]. Strong legislation is expected to regulate the presence of this transgenic animal in foods and environmental samples [ 252 , 253 ] and, consequently, reliable and sensitive methods for its detection will be required by regulatory and scientific agencies worldwide [ 245 , 254 ].…”

Section: Food Analysis and Traceabilitymentioning

confidence: 99%

Forensic genetics and genomics: Much more than just a human affair

et al. 2017

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While traditional forensic genetics has been oriented towards using human DNA in criminal investigation and civil court cases, it currently presents a much wider application range, including not only legal situations sensu stricto but also and, increasingly often, to preemptively avoid judicial processes. Despite some difficulties, current forensic genetics is progressively incorporating the analysis of nonhuman genetic material to a greater extent. The analysis of this material—including other animal species, plants, or microorganisms—is now broadly used, providing ancillary evidence in criminalistics in cases such as animal attacks, trafficking of species, bioterrorism and biocrimes, and identification of fraudulent food composition, among many others. Here, we explore how nonhuman forensic genetics is being revolutionized by the increasing variety of genetic markers, the establishment of faster, less error-burdened and cheaper sequencing technologies, and the emergence and improvement of models, methods, and bioinformatics facilities.

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Section: Food Analysis and Traceabilitymentioning

confidence: 99%

Forensic genetics and genomics: Much more than just a human affair

et al. 2017

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“…The primer pair and probe targeting GH1 were used as described previously [1,2]. The primer pair and probe targeting 18S rDNA were designed using Primer Express Software (Thermo Fisher Scientific, Version 3.0.1), based on the sequence given by NCBI ( Salmo salar 18S ribosomal RNA gene, partial sequence, GenBank accession no.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…The data provide information concerning DNA yield and purification efficiency from various processed salmon commodities that were produced worldwide and applicability of developed GH1 detection method [1] that was more sensitive than the original method described [2] to the commodities.…”

mentioning

confidence: 99%

Data representing applicability of developed growth hormone 1 (GH1) gene detection method for detecting Atlantic salmon (Salmo salar) at high specificity to processed salmon commodities

Soga¹,

Nakamura²,

Ishigaki³

et al. 2019

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This article is referred to the research article entitled “Development of a novel method for specific detection of genetically modified Atlantic salmon, AquAdvantage, using real-time polymerase chain reaction” by Soga et al. (2020).Applicability of the developed growth hormone 1 (GH1) and 18S ribosomal DNA (18S rDNA) detection methods using real-time polymerase chain reaction (PCR) for detecting Atlantic salmon (Salmo salar) to processed food commodities was examined. DNAs extracted and purified from 24 commodities labelled to include salmon as an ingredient were used as template. Yield and purity of DNAs obtained and Cq values from real-time PCR analyses were provided.

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“…An event-specific multiplex PCR assay was developed to assess six transgenic animal lines (GM human lactoferrin cattle, GM human lysozyme cattle, GM human α-lactalbumin cattle, myostatin knockout pigs, GM phytase pigs, and GM v-3 fatty acid desaturase pigs) [ 14 ]. A real-time PCR method specific for the Atlantic salmon (Salmo salar) growth hormone gene (GH1) was developed for the detection and quantification of S. salar L. DNA and Salmonidae ingredients in commercial foods [ 15 ]. Osamu et al (2013) developed a real-time PCR method targeting the hEpo gene to test the GM content originating from GM chickens carrying the hEpo gene in raw chicken meat and processed foods [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

An Event-Specific Real-Time PCR Method for Measuring Transgenic Lysozyme Goat Content in Trace Samples

Cui

Liu

et al. 2021

Foods

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Lysozymes are used in sterilisation, antisepsis, dairy additives, inflammation, and cancer. One transgenic goat line expressing high levels of human lysozyme (hLZ) in goat milk has been developed in China. Herein, we established an event-specific real-time polymerase chain reaction (real-time PCR) method to detect the transgenic hLZ goat line. The developed method has high specificity, sensitivity and accuracy, and a wide quantitative dynamic range. The limit of detection and limit of quantification was 5 and 10 copies per reaction, respectively. The practical sample analysis results showed that the method could identify and quantify transgenic lysozyme content in trace samples in routine lab analyses. Furthermore, the potential applicability in risk assessment, such as molecular characterisation and gene horizontal transfer, was confirmed. We believe that this method is suitable for the detection of transgenic hLZ goat line and its derivate.

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A new specific reference gene based on growth hormone gene (GH1) used for detection and relative quantification of Aquadvantage® GM salmon ( Salmo salar L.) in food products

Cited by 13 publications

References 27 publications

Forensic genetics and genomics: Much more than just a human affair

Forensic genetics and genomics: Much more than just a human affair

Data representing applicability of developed growth hormone 1 (GH1) gene detection method for detecting Atlantic salmon (Salmo salar) at high specificity to processed salmon commodities

An Event-Specific Real-Time PCR Method for Measuring Transgenic Lysozyme Goat Content in Trace Samples

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