A new spectrofluorimetric method for determination of losartan potassium in rabbit plasma and its application to pharmacokinetic study

Demirkaya-Miloglu, Fatma; Yaman, Mehmet Emrah; Kadıoğlu, Yücel

doi:10.1002/bio.2689

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…Many techniques have been published describing the analysis of LOS either in the pure form, dosage forms, or in biological fluids. These techniques include high‐performance liquid chromatography (HPLC), spectrofluorimetry, spectrophotometry, high‐performance thin layer chromatography (HPTLC), and capillary electrophoresis …”

Section: Introductionmentioning

confidence: 99%

First‐derivative synchronous spectrofluorimetric method for estimation of losartan potassium and atorvastatin in their pure forms and in tablets

et al. 2020

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Losartan potassium (LOS) and atorvastatin (ATR) are used in combination for longterm treatment of stroke and for treatment of hypertension with high-level cholesterol. Both drugs were simultaneously determined and validated using a novel, easy, fast, and economical first-derivative synchronous fluorescence spectroscopic method. Methanol was used as the solvent for both drugs at a Δλ 80 nm and with a scanning rate of 600 nm/min. Peaks were determined as at 288.1 nm and 263.6 nm for LOS and ATR, respectively. The proposed method was validated according to International Conference on Harmonization guidelines and, subsequently, the developed method was applicable to the analysis of the two compounds in their different formulations without interference from each other. Amplitude-concentration plots were rectilinear over the concentration ranges 1.0-10.0 μg/ml and 0.5-5.0 μg/ml for LOS and ATR, respectively. Detection limits were found to be 0.096 μg/ml and 0.030 μg/ml and quantitation limits were 0.291 μg/ml and 0.093 μg/ml for LOS and ATR, respectively. The proposed method was successfully applied to the analysis of both compounds in synthetic mixtures and in laboratory-prepared tablets. These results were in accordance with the results acquired using the comparison method, high-performance liquid chromatography. K E Y W O R D Satorvastatin, losartan potassium, prepared tablets, synchronous fluorescence spectroscopy

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Section: Introductionmentioning

confidence: 99%

First‐derivative synchronous spectrofluorimetric method for estimation of losartan potassium and atorvastatin in their pure forms and in tablets

et al. 2020

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“…Because of the unpredictable idea of the matrix (regularly plasma or urine) and the requirement for high affectability to take note of concentrations after low dose administration over a significant time, PK is frequently examined utilizing the fluorescence spectrometry procedure. [10][11][12] Presently, there is an interest for the utilization of high sensitivity fluorescence spectrometry for microdosing analysis, which is viewed as a promising option in substitute to animal investigation. 10,11 Pilot research in our lab indicated the native fluorescence property of RAV.…”

Section: Introductionmentioning

confidence: 99%

“…[10][11][12] Presently, there is an interest for the utilization of high sensitivity fluorescence spectrometry for microdosing analysis, which is viewed as a promising option in substitute to animal investigation. 10,11 Pilot research in our lab indicated the native fluorescence property of RAV. The additional evaluation of this occurrence prompted the recommendation that the utilization of fluorescence spectrometry could be one of the suitable choices for the quantification of RAV in rat plasma.…”

Section: Introductionmentioning

confidence: 99%

<p>Validated Microwell-Based Spectrofluorimetric Method for Quantification of Ravidasvir (New Anti-Chronic Hepatitis C Virus-GT4) in Rat Plasma and Its Application to Pharmacokinetic Study</p>

Hefnawy

Al‐Shehri

Al-Rashood

et al. 2020

DDDT

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Background: Ravidasvir (RAV) has been regarded as a potent new NS5A inhibitor with a magnificent safety and tolerability in the management of genotype 4 hepatitis C virus (HCV) patients. Suitable analytical techniques are needed for the measurement of RAV in different biological matrices. Methods: We have developed a fast, sensitive and economical 96-microwell-based spectrofluorimetric technique combined with one-step protein precipitation extraction strategy for the measurement of RAV in rat plasma. Results: Under the optimum conditions, the direct relationship in rat plasma was accomplished between the RAV concentrations and the fluorescence (FL) intensity in a scope of 2.5-200 ng/mL with 0.9998 and 0.9999 for the quantification and correlation coefficients, respectively. The lower limit of detection (LLOD) was 0.840 ng/mL and this demonstrates the high sensitivity of the proposed assay. The accuracy (RE%) ranged from 95.34% to 102.29%, and the precision (RSD%) was less than 3.59%. The recovery was ranged from 93.12% to 96.26%. The stability of RAV in rat plasma was carried out and established its good stability in the range of room conventional temperature and at long-term stability (−80° C, 30 days). The developed technique was validated as stated by the United States Food and Drug Administration (US-FDA) guidelines for bioanalytical technique verification. Conclusion: The approved technique was effectively applied for a pharmacokinetic (PK) study after single oral gavage administration of RAV at a dose of 35 mg/kg and it could be presumed that the proposed assay can be applied to clinical trials.

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“…Following oral administration, losartan is well absorbed with a systemic bioavailability of approximately 33% and the mean peak plasma concentrations of losartan and its metabolite are reached in 1-2 h and in 3-4h, respectively 5 . The determination of losartan potassium in human urine 6 and rabbit plasma 7 , and human plasma 8,9 were carried out.…”

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confidence: 99%

“…Although the losartan determination and pharmacokinetic study were carried out [7][8][9] , the interaction of losartan with serum albumin was barely studied. To our knowledge, there is only a report for studying the interaction of LP at the binding sites of bovine serum albumin by equilibrium dialysis method in presence or absence of palmitic acid using ranitidine and diazepam as site-1 and site-2 specific probe, respectively 11 .…”

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confidence: 99%

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2016

IJPSR

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ABSTRACT:The interaction of losartan potassium (LP) with bovine serum albumin (BSA) was studied by fluorescence quenching in combination with UV-Vis spectroscopic method under near physiological conditions. The fluorescence quenching rate constants and binding constants for BSA-LP system were determined at different temperatures. The fluorescence quenching of BSA by LP is due to static quenching and energy transfer. The results of thermodynamic parameters, ∆H (-134.3 kJ mol ) and ∆G (-24.52 to -20.83 kJ mol -1 ), indicated that van der Waals interaction and hydrogen bonding played a major role for LP-BSA association. The competitive experiments demonstrated that the primary binding site of LP on BSA was located at site II in sub-domain IIIA of BSA. The distance between LP and a tryptophane unit was estimated to be 3.183 nm based on the Förster resonance energy transfer theory. The binding constant (Ka) of BSA-LP at 298K was 1.932×104 L mol -1 . Synchronous fluorescence and three-dimensional fluorescence studies showed that the presence of LP could change the conformation of BSA during the binding process.

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A new spectrofluorimetric method for determination of losartan potassium in rabbit plasma and its application to pharmacokinetic study

Cited by 7 publications

References 21 publications

First‐derivative synchronous spectrofluorimetric method for estimation of losartan potassium and atorvastatin in their pure forms and in tablets

First‐derivative synchronous spectrofluorimetric method for estimation of losartan potassium and atorvastatin in their pure forms and in tablets

<p>Validated Microwell-Based Spectrofluorimetric Method for Quantification of Ravidasvir (New Anti-Chronic Hepatitis C Virus-GT4) in Rat Plasma and Its Application to Pharmacokinetic Study</p>

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