A new spin on an old model: In vivo evaluation of disease progression by magnetic resonance imaging with respect to standard inflammatory parameters and histopathology in the adjuvant arthritic rat

Jacobson, Peer B.; Morgan, Sheryl J.; Wilcox, Denise; Nguyen, Phong; Ratajczak, Christine A.; Carlson, Richard P.; Harris, Richard R.; Nuss, Merrill E.

doi:10.1002/1529-0131(199910)42:10<2060::aid-anr6>3.0.co;2-l

Cited by 49 publications

(31 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Adjuvant arthritis is very similar to human RA both in pathological and serological changes. 25) In this study, we found that all rats swelled and appeared red in the injected ankle on day 2, after complete Freund adjuvant injection. On day 7, the injected rats displayed secondary in‰ammation on the left hindpaw and forelimbs.…”

Section: Discussionsupporting

confidence: 50%

Anti-arthritic Active Fraction of <i>Capparis Spinosa</i> L. Fruits and Its Chemical Constituents

Xin

et al. 2011

YAKUGAKU ZASSHI

View full text Add to dashboard Cite

The aim of this study was to ascertain the anti-arthritic active fraction of Capparis spinosa L. (Capparidaceae) fruits and its chemical constituents. The adjuvant arthritic rat model was developed to evaluate the anti-arthritic eŠects of diŠerent fractions of ethanol extraction from C. spinosa L. The fraction eluted by ethanol-water (50：50, v/v) had the most signiˆcant anti-arthritic activity. The chemical constituents of this fraction were therefore studied; seven known compounds were isolated and identiˆed as: (1) P-hydroxy benzoic acid; (2) 5-(hydroxymethyl) furfural; (3) bis (5-formylfurfuryl) ether; (4) daucosterol; (5) a-D-fructofuranosides methyl; (6) uracil; and (7) stachydrine.

show abstract

Section: Discussionsupporting

confidence: 50%

Anti-arthritic Active Fraction of <i>Capparis Spinosa</i> L. Fruits and Its Chemical Constituents

Xin

et al. 2011

YAKUGAKU ZASSHI

View full text Add to dashboard Cite

show abstract

“…Evaluation of antirheumatic drugs on adjuvant-induced arthritis (AIA) has been performed on the basis of indicators such as paw swelling, clinical score, histopathology, X-radiography and recently magnetic resonance imaging (MRI) [13,14]. An antimetabolite, MTX has been reported to be effective in suppressing development of AIA [15 -17].…”

Section: Introductionmentioning

confidence: 99%

FK506 is superior to methotrexate in therapeutic effects on advanced stage of rat adjuvant-induced arthritis

et al. 2001

View full text Add to dashboard Cite

show abstract

“…The established model exhibits pronounced soft tissue and synovial inflammation between day 16 and 30 and is accompanied by a marked progression of periosteal reactions, pannus formation, internal bone inflammation, fibrosis in the joints, and end-stage ankylosis (Weichman, 1989). Classical paw edema measurements were made as well as examinations of paw architecture using MRI techniques described by us (Jacobson et al, 1999). Daily oral doses of ABT-963 from days 14 to 28 gave significant inhibition of paw swelling as measured by plethysmography and MRI.…”

Section: Cox-2 Inhibitors 909mentioning

confidence: 99%

“…Paw edema was evaluated for significance from control adjuvant edema using Dunnett's multiple comparison test where p Ͻ 0.05 was considered statistically significant. MRI images were taken at initiation of dosing and after the last dose (Jacobson et al, 1999).…”

mentioning

confidence: 99%

ABT-963 [2-(3,4-Difluoro-phenyl)-4-(3-hydroxy-3-methyl-butoxy)-5-(4-methanesulfonyl-phenyl)-2H-pyridazin-3-one], A Highly Potent and Selective Disubstituted Pyridazinone Cyclooxgenase-2 Inhibitor

Harris

Black²,

Surapaneni³

et al. 2004

J Pharmacol Exp Ther

Self Cite

View full text Add to dashboard Cite

Nonsteriodal anti-inflammatory drugs (NSAIDs) are efficacious for the treatment of pain associated with inflammatory disease. Clinical experience with marketed selective cyclooxygenase-2 (COX-2) inhibitors (celecoxib, rofecoxib, and valdecoxib) has confirmed the utility of these agents in the treatment of inflammatory pain with an improved gastrointestinal safety profile relative to NSAID comparators. These COX-2 inhibitors belong to the same structural class. Each contains a core heterocyclic ring with two appropriately substituted phenyl rings appended to adjacent atoms. Here, we report the identification of vicinally disubstituted pyridazinones as potent and selective COX-2 inhibitors. The lead compound in the series, ABT-963 [2-(3,4-difluoro-phenyl)-4-(3-hydroxy-3-methyl-butoxy)-5-(4-methanesulfonyl-phenyl)-2H-pyridazin-3-one], has excellent selectivity (ratio of 276, COX-2/COX-1) in human whole blood, improved aqueous solubility compared with celecoxib and rofecoxib, high oral anti-inflammatory potency in vivo, and gastric safety in the animal studies. After oral administration, ABT-963 reduced prostaglandin E 2 production in the rat carrageenan air pouch model (ED 50 of 0.4 mg/kg) and reduced the edema in the carrageenan induced paw edema model with an ED 30 of 1.9 mg/kg. ABT-963 dose dependently reduced nociception in the carrageenan hyperalgesia model (ED 50 of 3.1 mg/kg). After 14 days of dosing in the adjuvant arthritis model, ABT-963 had an ED 50 of 1.0 mg/kg in reducing the swelling of the hind paws. Magnetic resonance imaging examination of the diseased paws in the adjuvant model showed that ABT-963 significantly reduced bone loss and soft tissue destruction. ABT-963 is a highly selective COX-2 inhibitor that may have utility in the treatment of the pain and inflammation associated with arthritis.

show abstract

A new spin on an old model: In vivo evaluation of disease progression by magnetic resonance imaging with respect to standard inflammatory parameters and histopathology in the adjuvant arthritic rat

Abstract: MRI can be used to noninvasively detect, monitor, and quantify the chronic synovitis and progressive destruction of soft tissue and bone in live AIA rats, thereby improving the ability to evaluate disease progression in this preclinical animal model of rheumatoid arthritis.

Cited by 49 publications

References 36 publications

Anti-arthritic Active Fraction of <i>Capparis Spinosa</i> L. Fruits and Its Chemical Constituents

Anti-arthritic Active Fraction of <i>Capparis Spinosa</i> L. Fruits and Its Chemical Constituents

FK506 is superior to methotrexate in therapeutic effects on advanced stage of rat adjuvant-induced arthritis

ABT-963 [2-(3,4-Difluoro-phenyl)-4-(3-hydroxy-3-methyl-butoxy)-5-(4-methanesulfonyl-phenyl)-2H-pyridazin-3-one], A Highly Potent and Selective Disubstituted Pyridazinone Cyclooxgenase-2 Inhibitor

Contact Info

Product

Resources

About