A New Strategy to Reduce Amyloid Deposition using Peptide-Imprinted Membranes

Cristallini, Caterina; Bellotti, Elena; Spezia, F.; Rosellini, Elisabetta; Cascone, Maria Grazia; Barbani, Niccoletta

doi:10.5301/jabfm.5000288

Cited by 2 publications

(3 citation statements)

References 18 publications

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“…According to the binding amount of each surrogate peptide (Figure 5), the MIPs possessed a high degree of cross-reactivity for all these three peptides, while the NIPs showed low binding values. 41 The values of Q max are 1.04 ± 0.05 μmol/g for peptide-α, 0.64 ± 0.05 μmol/g for peptide-β, and 0.71 ± 0.03 μmol/g for peptide-γ, which were approximately 20% of that of the template. Interestingly, the maximum adsorption capacity decreases with the increase in the number of amino acids in surrogate peptide sequence (i.e., peptide-α (13aa) > peptide-γ (17aa) > peptide-β (18aa)).…”

Section: ■ Results and Discussionmentioning

confidence: 89%

“…Most importantly, we studied the recognition ability of the synthesized MIPs to peptide-α, β, and γ because cross-reactivity is the essential feature for this study as mentioned earlier. According to the binding amount of each surrogate peptide (Figure ), the MIPs possessed a high degree of cross-reactivity for all these three peptides, while the NIPs showed low binding values . The values of Q max are 1.04 ± 0.05 μmol/g for peptide-α, 0.64 ± 0.05 μmol/g for peptide-β, and 0.71 ± 0.03 μmol/g for peptide-γ, which were approximately 20% of that of the template.…”

Section: Resultsmentioning

confidence: 99%

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Simultaneous Detection of Human C-Terminal p53 Isoforms by Single Template Molecularly Imprinted Polymers (MIPs) Coupled with Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)-Based Targeted Proteomics

2018

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Abnormal expression of C-terminal p53 isoforms α, β, and γ can cause the development of cancers including breast cancer. To date, much evidence has demonstrated that these isoforms can differentially regulate target genes and modulate their expression. Thus, quantification of individual isoforms may help to link clinical outcome to p53 status and to improve cancer patient treatment. However, there are few studies on accurate determination of p53 isoforms, probably due to sequence homology of these isoforms and also their low abundance. In this study, a targeted proteomics assay combining molecularly imprinted polymers (MIPs) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for simultaneous quantification of C-terminal p53 isoforms. Isoform-specific surrogate peptides (i.e., KPLDGEYFTLQIR (peptide-α) for isoform α, KPLDGEYFTLQDQTSFQK (peptide-β) for isoform β, and KPLDGEYFTLQMLLDLR (peptide-γ) for isoform γ) were first selected and used in both MIPs enrichment and mass spectrometric detection. The common sequence KPLDGEYFTLQ of these three surrogate peptides was used as single template in MIPs. In addition to optimization of imprinting conditions and characterization of the prepared MIPs, binding affinity and cross-reactivity of the MIPs for each surrogate peptide were also evaluated. As a result, a LOQ of 5 nM was achieved, which was >15-fold more sensitive than that without MIPs. Finally, the assay was validated and applied to simultaneous quantitative analysis of C-terminal p53 isoforms α, β, and γ in several human breast cell lines (i.e., MCF-10A normal cells, MCF-7 and MDA-MB-231 cancer cells, and drug-resistant MCF-7/ADR cancer cells). This study is among the first to employ single template MIPs and cross-reactivity phenomenon to select isoform-specific surrogate peptides and enable simultaneous quantification of protein isoforms in LC-MS/MS-based targeted proteomics.

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Section: ■ Results and Discussionmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Simultaneous Detection of Human C-Terminal p53 Isoforms by Single Template Molecularly Imprinted Polymers (MIPs) Coupled with Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)-Based Targeted Proteomics

2018

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“…In addition to the mentioned types of molecularly imprinted membranes (MIMs), there are other particular membranes that will be discussed based on their particularities. In this category can be placed imprinted nanofilms and thin films, − flexible MIMs, − (hydro)/(cryo)gel MIMs, − biocompatible MIMs, − nanofiber mats, ,,− and even supported liquid membrane-protected molecularly imprinted beads. − …”

Section: Molecularly Imprinted Nanofiber Membranes (Minfms)mentioning

confidence: 99%

Molecularly Imprinted Membranes: Past, Present, and Future

2016

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More than 80 years ago, artificial materials with molecular recognition sites emerged. The application of molecular imprinting to membrane separation has been studied since 1962. Especially after 1990, such research has been intensively conducted by membranologists and molecular imprinters to understand the advantages of each technique with the aim of constructing an ideal membrane, which is still an active area of research. The present review aims to be a substantial, comprehensive, authoritative, critical, and general-interest review, placed at the cross section of two broad, interconnected, practical, and extremely dynamic fields, namely, the fields of membrane separation and molecularly imprinted polymers. This review describes the recent discoveries that appeared after repeated and fertile collisions between these two fields in the past three years, to which are added the worthy acknowledgments of pioneering discoveries and a look into the future of molecularly imprinted membranes. The review begins with a general introduction in membrane separation, followed by a short theoretical section regarding the basic principles of mass transport through a membrane. Following these general aspects on membrane separation, two principles of obtaining polymeric materials with molecular recognition properties are reviewed, namely, molecular imprinting and alternative molecular imprinting, followed the methods of obtaining and practical applications for the particular case of molecularly imprinted membranes. The review continues with insights into molecularly imprinted nanofiber membranes as a promising, highly optimized type of membrane that could provide a relatively high throughput without a simultaneous unwanted reduction in permselectivity. Finally, potential applications of molecularly imprinted membranes in a variety of fields are highlighted, and a look into the future of membrane separations is offered.

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A New Strategy to Reduce Amyloid Deposition using Peptide-Imprinted Membranes

Abstract: This work represents a proof of concept regarding the development of a biocompatible polymer membrane capable of selectively removing amyloid beta peptide from the blood and consequently from the cerebrospinal fluid.

Cited by 2 publications

References 18 publications

Simultaneous Detection of Human C-Terminal p53 Isoforms by Single Template Molecularly Imprinted Polymers (MIPs) Coupled with Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)-Based Targeted Proteomics

Simultaneous Detection of Human C-Terminal p53 Isoforms by Single Template Molecularly Imprinted Polymers (MIPs) Coupled with Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)-Based Targeted Proteomics

Molecularly Imprinted Membranes: Past, Present, and Future

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