A New Surface Plasmon Resonance-Based Immunoassay for Rapid, Reproducible and Sensitive Quantification of  Pentraxin-3 in Human Plasma

Canovi, Mara; Lucchetti, Jacopo; Stravalaci, Matteo; Valentino, Sonia; Bottazzi, Barbara; Salmona, Mario; Bastone, Antonio; Gobbi, Marco

doi:10.3390/s140610864

Cited by 19 publications

(13 citation statements)

References 26 publications

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“…The concentration of IL-6, IL-8 and VEGF in the cell culture medium was measured using the Quantikine ELISA assay (R&D Systems). The concentration of PTX3 both in the conditioned medium of ARPE-19 cells and in the vitreous humor of AMD and non-AMD donors was assessed using an in-house developed ELISA assay (Canovi et al, 2014).…”

Section: Enzyme-linked Immunosorbent Assaysmentioning

confidence: 99%

Control of Complement Activation by the Long Pentraxin PTX3: Implications in Age-Related Macular Degeneration

et al. 2020

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Dysregulation of the complement system is central to age-related macular degeneration (AMD), the leading cause of blindness in the developed world. Most of the genetic variation associated with AMD resides in complement genes, with the greatest risk associated with polymorphisms in the complement factor H (CFH) gene; factor H (FH) is the major inhibitor of the alternative pathway (AP) of complement that specifically targets C3b and the AP C3 convertase. Long pentraxin 3 (PTX3) is a soluble pattern recognition molecule that has been proposed to inhibit AP activation via recruitment of FH. Although present in the human retina, if and how PTX3 plays a role in AMD is still unclear. In this work we demonstrated the presence of PTX3 in the human vitreous and studied the PTX3-FH-C3b crosstalk and its effects on complement activation in a model of retinal pigment epithelium (RPE). RPE cells cultured in inflammatory AMD-like conditions overexpressed the PTX3 protein, and up-regulated AP activating genes. PTX3 bound RPE cells in a physiological setting, however this interaction was reduced in inflammatory conditions, whereby PTX3 had no complement-inhibiting activity on inflamed RPE. However, on non-cellular surfaces, PTX3 formed a stable ternary complex with FH and C3b that acted as a “hot spot” for complement inhibition. Our findings suggest a protective role for PTX3 in response to complement dysregulation in AMD and point to a novel mechanism of complement regulation by this pentraxin with potential implications in pathology and pharmacology of AMD.

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Section: Enzyme-linked Immunosorbent Assaysmentioning

confidence: 99%

Control of Complement Activation by the Long Pentraxin PTX3: Implications in Age-Related Macular Degeneration

et al. 2020

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“…Surface plasmon resonance (SPR) was recently shown to be a useful technique for estimating affinity of malaria-specific antibodies, using the dissociation constant (k d ) 21 . SPR has been recommended for use in vaccine efficacy studies to get more accurate evaluations 37 and it has also been shown to be suitable for use due to its efficiency in point-of-care diagnosis 38 , 39 . Antibody levels as determined by ELISA were also compared with SPR-measured affinity to further validate the use of SPR in measurement of affinity in malaria studies.…”

Section: Introductionmentioning

confidence: 99%

Factors influencing the induction of high affinity antibodies to Plasmodium falciparum merozoite antigens and how affinity changes over time

Tijani

Reddy

Langer

et al. 2018

Sci Rep

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Understanding the functional characteristics of naturally acquired antibodies against P. falciparum merozoite antigens is crucial for determining the protective functions of antibodies. Affinity (measured as kd) of naturally acquired antibodies against two key targets of acquired immunity, EBA175 and PfRh2, was determined using Surface Plasmon Resonance (SPR) in a longitudinal survey in Nigeria. A majority of the participants, 79% and 67%, maintained stable antibody affinities to EBA175 and PfRh2, respectively, over time. In about 10% of the individuals, there was a reciprocal interaction with a reduction over time in antibody affinity for PfRh2 and an increase for EBA175. In general, PfRh2 elicited antibodies with higher affinity compared to EBA175. Individuals with higher exposure to malaria produced antibodies with higher affinity to both antigens. Younger individuals (5–15 years) produced comparable or higher affinity antibodies than adults (>15 years) against EBA175, but not for PfRh2. Correlation between total IgG (ELISA) and affinity varied between individuals, but PfRh2 elicited antibodies with a higher correlation in a majority of the participants. There was also a correlation between antibody inhibition of erythrocyte invasion by merozoites and PfRh2 affinity. This work gives new insights into the generation and maintenance of antibody affinity over time.

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“…43 Though the detection of D-dimer in human plasma is more challenging than that in buffer samples, our sensor still exhibits a stable, sensitive performance.…”

Section: Detection Of D-dimer In Human Plasmamentioning

confidence: 98%

Graphene Oxide-Assisted Surface Plasmon Resonance Biosensor for Simple and Rapid Determination of D-Dimer in Plasma

Wang¹,

Lu²,

Zhang³

et al. 2016

j nanosci nanotechnol

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In this article, a surface plasmon resonance (SPR) biosensor based on graphene oxide (GO) for simple and rapid determination of D-dimer in plasma is introduced. GO was conjugated onto the surface of the SPR chip surface via electrostatic interaction after the chip surface was activated with cysteamine. Subsequently, anti-D-dimer was covalently immobilized on the chip surface through binding between carboxylic groups on the GO surface and amine groups on the protein surface. Finally, label-free and rapid detection of D-dimer was carried out by the SPR biosensor via protein-protein interaction. After optimization, the concentration of GO was found to be 2 mg mL −1 . Meanwhile, this biosensor presented a response to D-dimer in the concentration range of 10-150 ng · mL −1 , and limit of detection (LOD) of the developed biosensor was determined to be as low as 5.08 ng · mL −1 .In addition, addition of albumin from human serum (HSA) to the anti-D-dimer-modified SPR chip revealed a negligible response, indicating a satisfactory immunological specificity. Furthermore, D-dimer detection was successfully conducted in different concentrations of human plasma. These results suggest that the GO-assisted SPR biosensor could be exploited for point-of-care diagnosis in the clinical laboratory in the future.

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A New Surface Plasmon Resonance-Based Immunoassay for Rapid, Reproducible and Sensitive Quantification of Pentraxin-3 in Human Plasma

Cited by 19 publications

References 26 publications

Control of Complement Activation by the Long Pentraxin PTX3: Implications in Age-Related Macular Degeneration

Control of Complement Activation by the Long Pentraxin PTX3: Implications in Age-Related Macular Degeneration

Factors influencing the induction of high affinity antibodies to Plasmodium falciparum merozoite antigens and how affinity changes over time

Graphene Oxide-Assisted Surface Plasmon Resonance Biosensor for Simple and Rapid Determination of D-Dimer in Plasma

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