A new synbiotic,Lactobacillus caseisubsp.caseitogether with dextran, reduces murine and human allergic reaction

Abstract

We studied the development of atopic dermatitis-like skin lesions in NC/Nga mice and the allergic symptoms and blood patterns of healthy volunteers during the cedar (Cryptomeria japonica) pollen season in Japan following oral administration of a new synbiotic, Lactobacillus casei subsp. casei together with dextran. The combination of L. casei subsp. casei and dextran significantly decreased clinical skin severity scores and total immunoglobulin E levels in sera of NC/Nga mice that had developed picryl chloride-induced and Dermatophagoides pteronyssinus crude extract-swabbed atopic dermatitis-like skin lesions. During the most common Japanese cedar pollen season, synbiotic L. casei subsp. casei and dextran in humans led to no significant changes in total nasal and ocular symptom scores, in the levels of cedar pollen-specific immunoglobulin E, interferon-gamma and thymus and activation regulated chemokine or in the number of eosinophils in sera, whereas the placebo group showed a tendency for increased levels of cedar pollen-specific immunoglobulin E, thymus and activation regulated chemokine and number of eosinophils, and a decrease in interferon-gamma levels. Thus, the oral administration of synbiotic L. casei subsp. casei together with dextran appears to be an effective supplement for the prevention and treatment of allergic reactions.

RETRACTED: Preventative and Therapeutic Role of Probiotics in Various Allergic and Autoimmune Disorders: An Up-to-Date Literature Review of Essential Experimental and Clinical Data

“…This synbiotic combination significantly decreased clinical skin severity scores induced by picryl chloride and total IgE levels in sera of NC/Nga mice. 61 Also, supplementation with KW3110 strain of lactic acid bacteria significantly attenuated the onset and exacerbation of atopic dermatitis-like skin lesions, accompanied by lower plasma IgE levels through its effects on IL-12 and IL-4 production in vitro. 62 Oral administration of Lactobacillus brevis significantly inhibited IgE production and ear swelling, and suppressed the development of dermatitis in a dose-dependent manner in atopic dermatitis model of NC/Nga mice.…”

In Vivo Selection To Identify Bacterial Strains with Enhanced Ecological Performance in Synbiotic Applications

“…Despite this potential, however, there are few in vivo human or animal studies providing evidence that prebiotics can be used to support specific probiotic strains. Unfortunately, most synbiotic studies, including work in rats (44)(45)(46)(47)(48)(49), mice (50)(51)(52), pigs (53)(54)(55)(56)(57), chickens (58,59), and humans (60), did not employ strainspecific detection methods and therefore did not provide information on the potential synergism between pre-and probiotics. Of the in vivo studies that did discriminate the probiotic strain, most still did not demonstrate that in vivo performance could be enhanced by a prebiotic.…”

Clinical and Histopathological Evaluation of Dermatophagoides farinae-Induced Dermatitis in NC/Nga Mice Orally Administered Bacillus subtilis

“…To prevent atopic dermatitis, L. johnsonii should be administered early in the weaning period in NC mice [5]. Oral administration of symbiotic L. casei was effective in preventing atopic lesions induced by 2,4,6-trinitro-1-chlorobenzene when dextran was administered simultaneously [11]. Pagnini et al demonstrated that to prevent the onset of intestinal inflammation by local stimulation of epithelial innate immune responses, a probiotic mixture containing Streptococcus thermophilus, Bifidobacterium infantis, L. acidophilus, and Lactobacillus spp.…”

“…On the other hand, Lactobacillus spp. administration has a limited effect in preventing or inhibiting the development of atopic dermatitis [5,11,12]. Bacillus subtilis is one of the bacteria used for probiotics, and it is commonly used as an effective probiotic for preventing enteric infections in both humans and animals [20].…”