A new system using Solanum tuberosum for the co-cultivation of Glomus intraradices and its potential for mass producing spores of arbuscular mycorrhizal fungi

Puri, Anshul; Adholeya, Alok

doi:10.1007/s13199-012-0213-z

Cited by 9 publications

(4 citation statements)

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“…This is due mainly to the fact that; (1) undesired contamination is hardly avoidable and the technique is technology and skill demanding ( Bago and Cano, 2005 ), (2) there are ethical and legal concerns, and (3) it is rather very hard to identify each genotype (even morphotype) hence, most if not all, AMF are not readily culturable ( Fortin et al, 2005 ). AMF momoxenic in vitro culturing uses transformed [using Rhizobium rhizogenes (Riker et al) Young et al] hairy roots as host owing to the fact that these hairy roots are better suited than the non-transformed hairy roots since they grow on hormone free media and without developing shoots and leaves ( Puri and Adholeya, 2013 ). Meanwhile, AMF monoxenic culture as it is practiced now could potentially be challenged with biosafety related issues.…”

Section: Amf Biotechnology For the Restoration Of Degraded Landsmentioning

confidence: 99%

The Potential Role of Arbuscular Mycorrhizal Fungi in the Restoration of Degraded Lands

2016

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Experiences worldwide reveal that degraded lands restoration projects achieve little success or fail. Hence, understanding the underlying causes and accordingly, devising appropriate restoration mechanisms is crucial. In doing so, the ever-increasing aspiration and global commitments in degraded lands restoration could be realized. Here we explain that arbuscular mycorrhizal fungi (AMF) biotechnology is a potential mechanism to significantly improve the restoration success of degraded lands. There are abundant scientific evidences to demonstrate that AMF significantly improve soil attributes, increase above and belowground biodiversity, significantly improve tree/shrub seedlings survival, growth and establishment on moisture and nutrient stressed soils. AMF have also been shown to drive plant succession and may prevent invasion by alien species. The very few conditions where infective AMF are low in abundance and diversity is when the soil erodes, is disturbed and is devoid of vegetation cover. These are all common features of degraded lands. Meanwhile, degraded lands harbor low levels of infective AMF abundance and diversity. Therefore, the successful restoration of infective AMF can potentially improve the restoration success of degraded lands. Better AMF inoculation effects result when inocula are composed of native fungi instead of exotics, early seral instead of late seral fungi, and are consortia instead of few or single species. Future research efforts should focus on AMF effect on plant community primary productivity and plant competition. Further investigation focusing on forest ecosystems, and carried out at the field condition is highly recommended. Devising cheap and ethically widely accepted inocula production methods and better ways of AMF in situ management for effective restoration of degraded lands will also remain to be important research areas.

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Section: Amf Biotechnology For the Restoration Of Degraded Landsmentioning

confidence: 99%

The Potential Role of Arbuscular Mycorrhizal Fungi in the Restoration of Degraded Lands

2016

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“…An alternative technology exploits the genetically modified host roots (Root Organ Culture, ROC) to mass production of viable, healthy, genetically pure and high-grade fungal propagules without any pathogenic contamination under in vitro sterile conditions (Declerck et al, 2005;Puri, Adholeya, 2013). The technology was transferred to industries and resulted in the development of commercialized products which are ready available to end users for application.…”

Section: Development Of Multi-component Microbial Inoculantsmentioning

confidence: 99%

Prospects for the use of multi-component symbiotic systems of the Legumes

Shtark

Zhukov

Sulima

et al. 2015

Ecological genetics

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legume-rhizobial symbiosis (lrs), arbuscular mycorrhiza (am) and associations with Plant growthPromoting bacteria (PgPb) implement nutritional and defensive functions in plant, improve soil fertility, and thus are appropriate to be used for sustainable crop production and soil restoration. based on synergism and evolutional commonality of the symbioses, we propose a multi-component plantmicrobe system with legume plant as a main component. advances obtained from simultaneous inoculation of legumes with various beneficial microbes are summarized. basic principles of legume breeding to improve effectiveness of interaction with a complex of the microbes along with problems and prospects for development of multi-microbial inoculants for legumes (and nonlegumes) are stated.Key words: C Legumes; legumerhizobia symbiosis; arbuscular mycorrhiza; rhizospheric and endophytic plant growth-promoting bacteria; microbial cooperation; plant breeding; symbiotic effectiveness; multi-component microbial inoculants.prospeCts for the use of Multi-CoMponent syMbiotiC systeMs of the leguMes Поступила в редакцию 11.03.2015 Принята к публикации 30.03.2015 IntroductIon Legumes (Fabaceae, Syn. Leguminosae), the key components of modern agricultural technologies, represent unique group of plants able to intimately interact with different rhizosphere microorganisms. They form nitrogen-fixing root nodules with rhizobial bacteria (Legume-Rhizobia Symbiosis, LRS) (Dilworth et al., 2008), both rhizospheric and endorhizospheric associations with Plant Growth-Promoting Bacteria (PGPB) (Lugtenberg, 2015; Schulz et al., 2006), and Arbuscular Mycorrhiza (AM) with fungi of phylum Glomeromycota (Smith, Read 2008) ( Fig. 1 a-f). These symbioses are beneficial for the host plant and its environment as well. They provide plant with nutrients (predominately nitrogen and phosphorus), protect it from biotic and abiotic stresses and improve soil structure and fertility. In return, the microbial partners acquire the carbon from photosynthates and convenient niches for their life and reproduction. The symbioses are controlled genetically by both plant and microbial partner and have evolutional commonality (Hartmann et al., 2009; Lugtenberg, 2015;Oldroyd, Downie, 2008; Ormeño-Orrillo et al., 2013;Provorov, Shtark, 2014;Sessitsch et al., 2002;Shtark et al., 2010 Shtark et al., , 2012Smith, Read 2008;Sprent, James, 2007).The application of microbial inoculants based on the various beneficial soil bacteria and fungi allows improving the crop productivity and decrease the use of mineral fertilizers and pesticides. The vast majority of commercial inoculants available at the moment are based on pure cultures of single microorganism, and only occasionally on their combinations, mainly due to the current procedures for governmental registration and certification of inoculants. Moreover, the use of microbial inoculants in legumes is dominated by an application of rhizobial bacteria to fix atmospheric nitrogen (Gianinazzi, Vosatka, 2004; IJdo et al., 2011; Lugtenb...

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“…However, different approaches and experimental procedures are frequently applied to monitor the extraradical development of the fungal symbiont, making it difficult to compare the performance of hairy roots as hosts across studies. Hairy roots from various plant species have been trialed to cultivate AMF in vitro (i.e., Bago and Cano, 2005 ; Puri and Adholeya, 2013 ). Among these, carrot ( Daucus carota ) roots are commonly used as a root organ for AM monoxenic cultures ( Cranenbrouck et al, 2005 ; Kokkoris and Hart, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

RocTest: A standardized method to assess the performance of root organ cultures in the propagation of arbuscular mycorrhizal fungi

et al. 2022

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Over the past three decades, root organ cultures (ROCs) have been the gold standard method for studying arbuscular mycorrhizal fungi (AMF) under in vitro conditions, and ROCs derived from various plant species have been used as hosts for AM monoxenic cultures. While there is compelling evidence that host identity can significantly modify AMF fitness, there is currently no standardized methodology to assess the performance of ROCs in the propagation of their fungal symbionts. We describe RocTest, a robust methodological approach that models the propagation of AMF in symbiosis with ROCs. The development of extraradical fungal structures and the pattern of sporulation are modeled using cumulative link mixed models and linear mixed models. We demonstrate functionality of RocTest by evaluating the performance of three species of ROCs (Daucus carota, Medicago truncatula, Nicotiana benthamiana) in the propagation of three species of AMF (Rhizophagus clarus, Rhizophagus irregularis, Glomus sp.). RocTest produces a simple graphical output to assess the performance of ROCs and shows that fungal propagation depends on the three-way interaction between ROC, AMF, and time. RocTest makes it possible to identify the best combination of host/AMF for fungal development and spore production, making it an important asset for germplasm collections and AMF research.

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A new system using Solanum tuberosum for the co-cultivation of Glomus intraradices and its potential for mass producing spores of arbuscular mycorrhizal fungi

Cited by 9 publications

References 50 publications

The Potential Role of Arbuscular Mycorrhizal Fungi in the Restoration of Degraded Lands

The Potential Role of Arbuscular Mycorrhizal Fungi in the Restoration of Degraded Lands

Prospects for the use of multi-component symbiotic systems of the Legumes

RocTest: A standardized method to assess the performance of root organ cultures in the propagation of arbuscular mycorrhizal fungi

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