A new taxonomic marker of nodule bacteria of the Rhizobium genus and its evolution

Зотов, В. С.; Пунина, Н. В.; Хапчаева, С. А.; Didovych, S. V.; Melnichuk, T.N.; Топунов, А. Ф.

doi:10.1134/s2079059713020111

Cited by 1 publication

(3 citation statements)

References 31 publications

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“…The phylogenetic relationships between closely related strains Bacilllus cereus group and Rhizobium leguminosarum had been successfully analyzed using this method [38,39]. The procedure of saAFLP comprises three steps: (1) that we can obtain 10-30 DNA fragments after the treatment; (3) using the one single-stranded adapter.…”

Section: Saaflp Analysismentioning

confidence: 99%

“…Then, we sequenced and provided cluster analysis for the hin-region that, possibly, played the role into host-plant specificity and virulence capacity [39] (Fig. 1b).…”

Section: (A)mentioning

confidence: 99%

“…We modified amplified fragment length polymorphism (AFLP) method developed before by Vos et al [37] and named new modification as saAFLP [38,39]. The phylogenetic relationships between closely related strains Bacilllus cereus group and Rhizobium leguminosarum had been successfully analyzed using this method [38,39].…”

Section: Saaflp Analysismentioning

confidence: 99%

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Identification and Diagnostics of Plant-Symbiotic and Phytopathogenic Bacteria

Пунина¹,

Remnev²,

Топунов³

2015

JAST-A

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Rapid and reliable diagnostics and identification of pathogenic and symbiotic bacteria are at the top of the agenda. In the first case, they are important to control and prevent crop damages, and thus reduce economic losses. In the second, it's necessary to design and monitor quality of biofertilizer to raise its effectiveness and crop capacity. Development of accurately, rapidly, technically and commercially accessible methods remains a critical problem for the bacteria with comprehensive phylogenetic structure. In this work, we investigated pathogenic Xanthomonas and Ralstonia and symbiotic Sinorhizobium. The aim of this investigation was to examine the applicability of the novel methods for phylogenetic study, identification and diagnostics of closely related species of these genera. The conventional phenotypic and genotypic (16S rRNA, gyrB) methods were applied as referents. Novel polymerase chain reaction (PCR)-based approaches, single-adapter amplified fragment length polymorphism (saAFLP) and comparative analyses of hin-region and Xcc0006-0007 sequences, were first employed for the investigations. Phenotypic tests, 16S rRNA and gyrB analysis distinguished bacteria at the genus level, but failed to identify them to the species robustly. The new methods identified bacteria at the inter-species level more precisely. This identification agreed with the accepted genera's classifications. The only exceptions were X. fuscans & X. citri and X. perforance & X. euvesicatoria which clustered together. The further outcome of this study was achieved hin-region-based genus-specific PCR primers for the express-diagnostics of the genera. Summary, these new methods can be applied for genome-based phylogeny investigations and as convenient and accurate tools for identification and routine laboratory diagnostics of these comprehensive genera.

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Section: Saaflp Analysismentioning

confidence: 99%

“…Then, we sequenced and provided cluster analysis for the hin-region that, possibly, played the role into host-plant specificity and virulence capacity [39] (Fig. 1b).…”

Section: (A)mentioning

confidence: 99%