A new technique for the analysis of endogenous mediators released following thermal injury

Yregård, L; Löwhagen, P.Hendén; Cassuto, Jean; Nilsson, Ulf; Lindblom, L; Räntfors, J; Tarnow, Peter

doi:10.1016/s0305-4179(00)00077-2

Cited by 11 publications

(6 citation statements)

References 28 publications

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“…A significant inhibition of PGF 2α release into the circulation was seen during systemic administration of lidocaine in dogs with cardiac arrhythmias (70). In a recent study using a technique allowing for the in vivo analysis of inflammatory mediators released post‐burn (71), a potent inhibition of PGE 1 and PGE 2 release was demonstrated when treating the burned skin in the intact animal with a topical local anesthetic cream (71), thus confirming an earlier report showing reduced PGE release from isolated pieces of gastric mucosa by lidocaine (67) (Fig. 3).…”

Section: Effects Of Local Anesthetics On Synthesis and Release Of Infsupporting

confidence: 76%

Anti‐inflammatory properties of local anesthetics and their present and potential clinical implications

Cassuto

Sinclair

Bonderovic

2006

Acta Anaesthesiol Scand

376

286

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Development of new local anesthetic agents has been focused on the potency of their nerve-blocking effects, duration of action and safety and has resulted in a substantial number of agents in clinical use. It is well established and well documented that the nerve blocking effects of local anesthetics are secondary to their interaction with the Na+ channels thereby blocking nerve membrane excitability and the generation of action potentials. Accumulating data suggest however that local anesthetics also possess a wide range of anti-inflammatory actions through their effects on cells of the immune system, as well as on other cells, e.g. microorganisms, thrombocytes and erythrocytes. The potent anti-inflammatory properties of local anesthetics, superior in several aspects to traditional anti-inflammatory agents of the NSAID and steroid groups and with fewer side-effects, has prompted clinicians to introduce them in the treatment of various inflammation-related conditions and diseases. They have proved successful in the treatment of burn injuries, interstitial cystitis, ulcerative proctitis, arthritis and herpes simplex infections. The detailed mechanisms of action are not fully understood but seem to involve a reversible interaction with membrane proteins and lipids thus regulating cell metabolic activity, migration, exocytosis and phagocytosis.

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Section: Effects Of Local Anesthetics On Synthesis and Release Of Infsupporting

confidence: 76%

Anti‐inflammatory properties of local anesthetics and their present and potential clinical implications

Cassuto

Sinclair

Bonderovic

2006

Acta Anaesthesiol Scand

376

286

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“…Lidocaine administration significantly secret IL-8 and IL-10 in cell culture experiments and this effect can mediate inhibition of NFkb via decrease IkB phosphorylation (Lang et al, 2009). In a recent study a potent inhibition of PGE 1 and PGE 2 release was demonstrated when treating the burned skin in the intact animal with a topical local anesthetic cream (Yregård et al, 2001), thus confirming an earlier report showing reduced PGE 2 release from isolated pieces of gastric mucosa by lidocaine (Goel et al, 1994). These inhibitory effects on PGE 2 , known to play a significant role in the mechanisms responsible for inflammatory pain, could account for some of the potent analgesic effects of intravenous lidocaine reported in burn patients (Jönsson et al, 1999, Cassuto andTarnow, 2003) and in patients having undergone surgery (Cassuto et al, 1995;Chen et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Lidocaine subgingival irrigation modulate the levels of prostaglandin E2 in gingival crevicular fluid after periodontal therapy

Camargo¹,

Fellows²,

Poiate³

et al. 2015

J. Dent. Oral Hyg.

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The aim of this study was to evaluate if lidocaine 2% with 1:100.000 epinephrine, used as subgingival irrigation, has anti-inflammatory effect after periodontal therapy. Seventeen patients were selected to this paired split mouth randomized, subject-blind study. Each patient had a minimum of two sites labeled and alleatory separated, both with probe depth ≥ 5 mm. Each site were separated in two groups: scaling and root planing + lidocaine 2% with 1:100.000 epinephrine used as subgingival irrigation (LD); and scaling and root planing + saline solution (SRP), both groups were blinded to the examiner. Clinical periodontal parameters were recorded: plaque index (PI), bleeding on probe (BOP), probing depth (PD), gingival recession (GR) and clinical attachment level (CAL). Levels of GCF-PGE 2 were analyzed by enzyme-linked immunosorbent. All parameters were recorded at baseline (1 and 3 months) after periodontal treatment. The results indicated reduction of PI, BOP, PD, CAL for LD and SRP groups and GCF-PGE 2 levels were reduced in LD group after 3 months periodontal therapy. The LD and SRP groups were equally efficacious to control the periodontal disease after 3 months. However, LD improved the reduction of PGE 2 levels and maintains the inhibitory anti-inflammatory effect on PGE 2 after 3 months of periodontal treatment.

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“…In mammals, axon degeneration is known to produce post-burn neuropathic-like pain (Fauerbach et al, 2005; Schneider et al, 2006; Wong and Turner, 2010), and expression of pro-inflammatory cytokines that induce an inflammatory response in the organism (Summer et al, 2008). In addition, these burn effects will probably induce macrophage activation and migration to the site of injury (Yregård et al, 2001; Shields et al, 2012; Rosenberg et al, 2012). Inflammation and neuropathic pain have been shown to activate a list of factors in key neuromodulatory regions in mammals, like dorsal root ganglia, spinal cord neurons and neurons of the brain.…”

Section: Discussionmentioning

confidence: 99%

“…Animal in vivo experimentation, above all in the field of pain and nociception, presents ethical and scientific limitations. Rats and mice are considered the most useful animal models in this context, being suitable for genetic manipulation and having a large choice of mutants already available (Nozaki-Taguchi and Yaksh, 1998; Yregård et al, 2001; Wang et al 2005; Mogil and Basbaum, 2011; Shields et al, 2012). Studies of skin burns in animal models showed how axons innervating the tissue could be severely affected either in the short-term period directly by the burns (deCamara et al, 1982; Summer et al, 2007), or by the scar tissue, that can heavily affect axons growth in the long-term (Summer et al, 2007; Morellini et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Extreme Thermal Noxious Stimuli Induce Pain Responses in Zebrafish Larvae

Malafoglia

Colasanti

Raffaeli

et al. 2013

Journal Cellular Physiology

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Exposing tissues to extreme high or low temperature leads to burns. Burned animals sustain several types of damage, from the disruption of the tissue to degeneration of axons projecting through muscle and skin. Such damage causes pain due to both inflammation and axonal degeneration (neuropathic-like pain). Thus, the approach to cure and alleviate the symptoms of burns must be twofold: rebuilding the tissue that has been destroyed and alleviating the pain derived from the burns. While tissue regeneration techniques have been developed, less is known on the treatment of the induced pain. Thus, appropriate animal models are necessary for the development of the best treatment for pain induced in burned tissues. We have developed a methodology in the zebrafish aimed to produce a new animal model for the study of pain induced by burns. Here we show that two events linked to the onset of burn-induced inflammation and neuropathic-like pain in mammals, degeneration of axons innervating the affected tissues and over-expression of specific genes in sensory tissues, are conserved from zebrafish to mammals.

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A new technique for the analysis of endogenous mediators released following thermal injury

Cited by 11 publications

References 28 publications

Anti‐inflammatory properties of local anesthetics and their present and potential clinical implications

Anti‐inflammatory properties of local anesthetics and their present and potential clinical implications

Lidocaine subgingival irrigation modulate the levels of prostaglandin E2 in gingival crevicular fluid after periodontal therapy

Extreme Thermal Noxious Stimuli Induce Pain Responses in Zebrafish Larvae

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