A new thermostable and organic solvent-tolerant lipase from Aneurinibacillus thermoaerophilus strain HZ

Masomian, Malihe; Rahman, Raja Noor Zaliha Raja Abdul; Salleh, Abu Bakar; Basri, Mahiran

doi:10.1016/j.procbio.2012.11.002

Cited by 61 publications

(54 citation statements)

References 34 publications

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“…Screening oil contaminated soils, industrial effluents and salty habitats for lipase producing microbial strains has been introduced as the main step to obtain lipases harboring noticeable biochemical properties (Abdel-Fattah et al, 2012;Masomian et al, 2013). For example, Patel et al (2014) isolated an organic solvent-stable Pseudomonas sp.…”

Section: Resultsmentioning

confidence: 99%

“…All six applied solvents in the study by Yu et al (2010), demonstrated a positive effect on the catalytic activity of the recombinant lipase from Fervidobacterium nodosum Rt17-B1. On the other hand, in the study performed by Masomian et al (2013), it was found that among twelve tested organic solvents, 75 and 23% of the initial activity of lipase obtained from Aneurinibacillus thermoaerophilus were lost in the presence of dodecanol and xylene, respectively.…”

Section: Partial Purification Of Lipasementioning

confidence: 94%

“…These valuable biocatalysts have been widely used in various industries such as food (for example, hydrolysis of milk fat and cheese ripening in dairy foods), detergent, pulp and paper (papers with improved quality), fine chemicals (such as resolution of racemic acids and alcohols and regioselective acylations) and oleochemical manufacturing (Salameh and Wiegel, 2007;Sharma et al, 2011;AbdelFattah et al, 2012). Synthesis of biopolymers and biodiesel, resolution of enantio-specific pharmaceuticals and production of agro-chemicals and flavoring compounds are among other biotechnological applications of lipases (Soliman et al, 2007;Yu et al, 2010;Andualema and Gessesse, 2012 [154][155][156][157][158][159][160][161][162][163][164]2015 Because of high demands for the lipases tolerant to extreme conditions, screening of the environments harboring halophilic and thermophilic lipase-producing microbial strains is still a main step for introducing lipolytic enzyme with remarkable properties (Daoud et al, 2013;Masomian et al, 2013). Extremophiles include microorganisms which are able to survive in harsh environmental conditions, such as extremely high or low temperatures, high concentration of salt, severe alkaline or acidic pH, low water activity and high pressure (Salameh and Wiegel, 2007;Moshfegh et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…(Daoud et al, 2013) and Aneurinibacillus thermoaerophilus strain HZ. (Masomian et al, 2013). Isolates of Bacillus genera (B. subtilis DR8806 and B. subtilis NS8) have been found to produce lipases active at alkaline pH and in elevated temperature conditions (Olusesan et al, 2011;Emtenani et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Ameri¹,

Shakibaie²,

Amirpour-R³

et al. 2015

Biotechnology

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A B S T R A C T A thermoalkalophilic lipase producing bacterial strain, identified asBacillus atrophaeus FSHM2 using 16S rDNA sequencing analysis was isolated from salty soil and its lipase was partially purified and characterized. The obtained results revealed that glucose, hazelnut oil, urea and calcium ion positively affected the lipase production by increasing the lipolytic activity to 13582.5, 6270, 4442 and 5505 U LG 1 , respectively compared to that of basal medium (4150 U LG 1 ). The partially purified lipase acted optimally at pH 9 and retained 88.2% of its initial activity after 1 h of incubation at 100°C. A two fold increase in the relative activity of the partially purified lipase was obtained in the presence of 4 M of NaCl. Application of the partially purified lipase for the synthesis of ethyl and methyl valerate in the organic solvent medium (xylene) resulted in 81.6 and 62.4% esterification, respectively, after 24 h of incubation.

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Section: Resultsmentioning

confidence: 99%

Section: Partial Purification Of Lipasementioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Ameri¹,

Shakibaie²,

Amirpour-R³

et al. 2015

Biotechnology

View full text Add to dashboard Cite

show abstract

“…Lipase activity has generally been proven to be calciumdependent, and the lipase from B. pumilis is an exception to this norm (Kim et al 2002). Aneurinibacillus, a genus that can be differentiated from Bacillus based on 16S rRNA sequence, also produces a thermostable and organic solvent-tolerant lipase (Masomian et al 2013).…”

Section: Sources Of Microbial Lipasesmentioning

confidence: 99%

An insight into microbial lipases and their environmental facet

Kanmani¹,

Aravind²,

Kumaresan³

2014

Int. J. Environ. Sci. Technol.

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Lipases are serine hydrolases that catalyze the hydrolysis and synthesis of esters formed from glycerol and long-chain fatty acids, by acting at the oil-water interface. Lipases from microbial sources have received heightened attention for an array of industrial applications, and these enzymes have been well exploited in the environmental sector as well. In this article, we present an overview of microbial lipase, including the microorganisms from which it could be produced; the application of recombinant DNA technology tools to produce lipase with enhanced properties, the effective use of waste materials as substrates for lipase production; the usage of statistical tools to efficiently optimize the production medium; lipase purification strategies; and the immobilization of the enzyme on a variety of support materials. The next section of the article provides a gist of its application in diversified spheres and focusses exclusively on the environmentally relevant ones. Lipasecatalyzed esterification, transesterification, and interesterification reactions, an emerging area of green chemistry; lipase-mediated in vitro biopolymer synthesis and degradation; and the application of lipase for remediating fat and oil constituents in wastewater are dealt with in-depth. When its full potential is harnessed, the enzyme could play a pivotal role in environmental management.

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Screening and characterization of a thermostable lipase from marineStreptomycessp. strain W007

Yuan

Lan

Xin

et al. 2015

Biotech and App Biochem

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A screening method along with the combination of genome sequence of microorganism, pairwise alignment, and lipase classification was used to search the thermostable lipase. Then, a potential thermostable lipase (named MAS1) from marine Streptomyces sp. strain W007 was expressed in Pichia pastoris X-33, and the biochemical properties were characterized. Lipase MAS1 belongs to the subfamily I.7, and it has 38% identity to the well-characterized Bacillus subtilis thermostable lipases in the subfamily I.4. The purified enzyme was estimated to be 29 kDa. The enzyme showed optimal temperature at 40 °C, and retained more than 80% of initial activity after 1 H incubation at 60 °C, suggesting that MAS1 was a thermostable lipase. MAS1 was an alkaline enzyme with optimal pH value at 7.0 and had stable activity for 12 H of incubation at pH 6.0-9.0. It was stable and retained about 90% of initial activity in the presence of Cu(2+) , Ca(2+) , Ni(2+) , and Mg(2+) , whereas 89.05% of the initial activity was retained when ethylene diamine tetraacetic acid was added. MAS1 showed the tolerance to organic solvents, but was inhibited by various surfactants. MAS1 was verified to be a triglyceride lipase and could hydrolyze triacylglycerol and diacylglycerol. The result represents a good example for researchers to discover thermostable lipase for industrial application.

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A new thermostable and organic solvent-tolerant lipase from Aneurinibacillus thermoaerophilus strain HZ

Cited by 61 publications

References 34 publications

Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

An insight into microbial lipases and their environmental facet

Screening and characterization of a thermostable lipase from marineStreptomycessp. strain W007

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