A new TLC bioautographic assay for qualitative and quantitative estimation of lipase inhibitors

Abstract

The developed method is rapid, simple, stable, and specific for screening and estimation of the potential lipase inhibitors.

Improved TLC Bioautographic Assay for Qualitative and Quantitative Estimation of Tyrosinase Inhibitors in Natural Products

“…The RSD values of resveratrol, rhodiolinin, kaempferol, and ascorbic acid were 16.32, 5.14, 1.17, and 5.90%, at three concentration levels, indicating that the RTIC value is relatively independent from their sample concentrations. Our current results confirmed again that the application of an area under the curve (AUC) for bioactivity estimation, expressed as a positive control equivalent, is an effective approach to assess the activity across different laboratories (Cheng et al ., ; Tang et al ., ). The order of RTIC values was kaempferol > ascorbic acid > rhodiolinin > resveratrol, with a smaller value associated with a stronger inhibitory activity.…”

“…In summary, our TLC bioautographic assay for tyrosinase inhibition has several advantages in common with previously described TLC bioautographic assays (Tang et al, 2016), such as tracking and locating active components in crude extracts, and avoiding the interference from solvents and reagents of the test system. In addition, with L-DOPA as the substrate, our developed TLC bioautographic assay for tyrosinase inhibitors has significantly higher sensitivity and lower consumption of enzyme than the previous TLC bioautographic method.…”

Recent Advances in Effect‐directed Enzyme Assays based on Thin‐layer Chromatography

“…Two TLC bioautographic procedures for detecting lipase inhibitors have already been introduced (Hassan, 2012;Bayineni et al 2014). Recently, a bioautographic assay with higher substrate specificity that allows a quantitative evaluation of lipase inhibitors has been developed (Tang et al, 2016). The assay is based on the reaction of lipase with β-naphthylmyristate and the subsequent formation of purple dye when β-naphthol reacts with FBB salt (Fig.…”

“…Bioautographic lipase assay. Lipase hydrolyses the substrate β-naphthyl myristate to yield β-naphthol, which in turn reacts with Fast Blue B (FBB) salt to give a purple-coloured diazonium dye(Tang et al, 2016).…”

Establishment of an effective TLC bioautographic method for the detection ofMycobacterium tuberculosisH37Ra phosphoglucose isomerase inhibition by phosphoenolpyruvate

“…In our opinion, there is a clear trend towards the use of the TLC bioautography technique as a method for screening active compounds against microorganisms rather than a method for the detection of enzyme activities or inhibitors. The use of TLC (bio)autography was reported for the screening of inhibitors of acetylcholinesterase [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] , butyrylcholinesterase 12,16,22,23,27 , dipeptidyl peptidase IV 28 , glucose-6-phosphate dehydrogenase 29 , a-and b-glucosidases [30][31][32][33][34] , lipase [35][36][37] , monoamine oxidases A and B 38,39 , DNA topoisomerase I 40 , tyrosinase [41][42][43] and xanthine oxidase 44 . To date, the use of the TLC-based autographic method for the detection of PGI inhibition has only been developed for a biocatalyst isolated from the reference E. coli strain ATCC (American Type Culture Collection) 25922 45 .…”