2011
DOI: 10.1021/es200455p
|View full text |Cite
|
Sign up to set email alerts
|

A New Transcriptional Effect Level Index (TELI) for Toxicogenomics-based Toxicity Assessment

Abstract: This study proposes and demonstrates the potential application of a new Transcriptional Effect Level Index (TELI) to convert the information-rich toxicogenomic data into integrated and quantitative endpoints. A library of transcriptional fusions of green fluorescent protein (GFP) that includes different promoters for 91 stress-related genes in E. coli K12, MG1655 is employed to evaluate the gene expression alteration induced by exposure to four nanomaterials (NMs), nano silver (nAg), nano titanium dioxide anat… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

3
106
0

Year Published

2013
2013
2023
2023

Publication Types

Select...
9

Relationship

3
6

Authors

Journals

citations
Cited by 69 publications
(109 citation statements)
references
References 34 publications
3
106
0
Order By: Relevance
“…The magnitude of gene expression changes was indicated by the absolute TELI value of each gene after a 2 h exposure (that considers both up- and downregulation as altered expression). 32 These profiles revealed the dynamic nature of toxicity resulted from the individual CECs as well as from a mixture of transformation products during the course of the degradation process. To further quantitatively illustrate the nature of toxicity changes during the transformation of the CECs, molecular endpoint 1/EC-TELI1.5 values for the individual stress response categories were calculated and are plotted in Figure 3.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…The magnitude of gene expression changes was indicated by the absolute TELI value of each gene after a 2 h exposure (that considers both up- and downregulation as altered expression). 32 These profiles revealed the dynamic nature of toxicity resulted from the individual CECs as well as from a mixture of transformation products during the course of the degradation process. To further quantitatively illustrate the nature of toxicity changes during the transformation of the CECs, molecular endpoint 1/EC-TELI1.5 values for the individual stress response categories were calculated and are plotted in Figure 3.…”
Section: Resultsmentioning
confidence: 99%
“…TELI is calculated by integrating induction factor values over time and indicates the cumulative transcriptional effect of a given gene over a certain exposure period. 32,41 TELI values can be determined for a single gene or for a number of genes representing a specific pathway or the entire stress response library. 32 TELI exhibited dose–response patterns for both a single gene and a gene ensemble.…”
Section: Materials and Methodsmentioning
confidence: 99%
“…To quantify the chemical-induced gene/protein expression level changes of a treatment, Transcriptional Effect Level Index (TELI) 35 for E. coli and Protein Effect Level Index (PELI) 36 for yeast were proposed and derived as quantitative molecular endpoints (details described in the SI, part 3). TELI and PELI quantify the accumulative altered gene or protein expression change over the exposure period for a given gene (TELI gene , PELI gene ), pathway (TELI pathway , PELI pathway ), or for the overall DNA damage and repair pathway ensemble library (TELI geno , PELI geno ).…”
Section: Materials and Methodsmentioning
confidence: 99%
“…27,28,40 On the basis of the function and nature of the proteins selected in this study that are essential in yeast cellular stress responses (Table S1, Supporting Information), the extent of up-regulation reflecting yeast cellular stress responses is quantified as follows. 27,28,40 …”
Section: Methodsmentioning
confidence: 99%