A New Type of Fluorescence Labeling of Nucleosides, Nucleotides and Oligonucleotides

The effect of overhanging nucleotides on fluorescence properties of hybridising oligonucleotides labelled with Alexa-488 and FAM fluorophores

“…Other mechanisms operating in parallel include hydrophobic interactions and proton coupled transfer which allow endergonic electron transfer to operate [7]. 0301 Various studies have demonstrated that probes labelled with a 5V fluorescein (FAM) can be quenched by the presence of guanine bases in the same strand [8] and in a complementary hybridising strand [4,5]. Quenching from guanine bases in a hybridising strand can be either in the complementary region [5,6] or part of a 3V overhang region that is adjacent to the fluorophore attachment site of the probe.…”

Synthesis of 3′-Sugar- and Base-Modified Nucleotides and Their Application as Potent Chain Terminators in DNA Sequencing

“…Reaction of cytosines with sodium hydrogen sulfite resulted in 5,6-dihydrocytosine-6-sulfonate [22], which could readily be substituted at NH 2 ÀC(4) with a primary amine [23] [24]. Treatment of cytidines with 1-(phenoxycarbonyl)-1H-tetrazole resulted in conversion of the exocyclic NH 2 group to the corresponding urethane, which could be used to introduce an amino linker under formation of an urea bond [25]. Starting from uracil derivatives, the C(4) position could be activated with 1H-1,2,4-triazole as well as with phosphoroxychloride [26] or 4-chlorophenyl phosphorodichloridate [27].…”

Study on the Application of Gatifloxacin-A Novel Fluorescent System

“…Quinolone antibiotics can be applied to the human body and can also be applied to animals. The antibacterial agent has the characteristics of wide antibacterial spectrum and strong activity, little side effect, no cross-resistance with other drugs, and has been widely used in animal husbandry and aquaculture Application, can be used for prevention and treatment of diseases [1][2][3][4][5] .There is an antibacterial drug called gatifloxacin, is a quinolone drugs, as humans gradually found that quinolones antibacterial effect is particularly good, so with quinolones synthesized more and more new structures, so for quinolones University drug testing methods are more and more important [6][7][8][9] .…”