A new UHPLC-HRMS metabolomics approach for the rapid and comprehensive analysis of phenolic compounds in blueberry, raspberry, blackberry, cranberry and cherry fruits

Underutilized Canadian wild berries as potential sources of lipophilic bioactive compounds with antihypertensive properties

“…When comparing these results, it is important to note that ACE1 inhibitory activity is often linked to the presence of bioactive compounds, including highly abundant polyphenols, fatty acids, phytosterols, terpenes, bioactive peptides, etc ., which can vary among different berries. 1,22 The higher ACE1 inhibitory activity in chokeberries aligns with previous research highlighting these berries as potential contributors to cardiovascular health. 20,50 In this study, the ACE1 inhibitory activity of the lipophilic fraction of Canadian wild berries was assessed using an in vitro method.…”

“…Some components within the berry extract may have intrinsic inhibitory effects on the ACE1 enzyme, leading to a lower result. 1,22 Therefore, considering the complex composition of the berry and its potential interactions with the ACE1 enzyme is vital. In addition, the extract might exhibit inhibitory effects at higher concentrations, while at lower concentrations, the effects are not pronounced or may even appear negative.…”

“…The methodology for extracting berries followed the approach described in Kodikara et al (2023). 22 Briefly, freeze-dried berry samples (500 mg) were placed in clean 15 mL falcon tubes, and a solvent mixture (6 mL) of isopropanol : water (80 : 20, v/v). The mixture was facilitated with thorough mixing and extraction on a rotary shaker at 60 rpm for 30 min, followed by sonication at 30 °C for 30 min.…”

“…While a significant amount of research has focused on the lipophilic compounds in commonly consumed berries, comprehensive data are scarce, such as fatty acid profiles, phytosterol content, terpenes composition, lipid peroxidation susceptibility, and ACE inhibitory activity of various underutilized wild berries. 22 Existing research is limited to a few common berry types, such as blueberries, raspberries, and seabuckthorn, leaving numerous other species unexplored. 23,24 When considering the berry types, seabuckthorn is the most widely studied berry type among the buckthorn species for its fatty acid and phytosterol content.…”

Critical Review of Selected Analytical Platforms for GC-MS Metabolomics Profiling—Case Study: HS-SPME/GC-MS Analysis of Blackberry’s Aroma

“…With their bioactive components, which include phenolic acids and flavonoids [27], they have strong antioxidant activity [28] and help prevent a variety of ailments. Up to now, blackberries have been the subject of extensive studies using liquid chromatography with mass spectrometry and multivariate data analysis [27][28][29][30]. To the best of our knowledge, this is the first time that a metabolomic approach has been used in the analysis of blackberry aroma, as well as the first time that the results of different platforms for preprocessing GC-MS results have been compared.…”