A new validated ultra performance liquid chromatographic method for determination of acyclovir

Abstract

Recently, ultra performance liquid chromatography (UPLC) has proven to be one of the most promising developments in the area of fast chromatographic separations, as it's been true to the objective of reducing analysis time and maintaining good efficiency. The present report describes development and validation of a new, rapid, and sensitive UPLC method with UV detection to quantify acyclovir in bulk sample using a Waters Acquity HSS T-3 (100 × 2.1 mm, 1.8 µm) column maintained at a temperature of 50 °C with a gradient elution consisting of 1.0% triethyl amine in water and acetonitrile at a flow rate of 0.4 mL/min and detected at 254 nm. Method was found to be selective, linear, accurate, and precise as per ICH guidelines. Detection and quantitation limits of drug were 3 and 8 ng/mL, respectively.

Determination of the Antiretroviral Drug Acyclovir in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

“…In particular, this approach offers similar efficiency as compared with chromatographic methods. 3–7 The behavior of the acyclovir peak is similar to the DNA-copper peak. 22 Also, further studies using diluted alkaline solution as the supporting electrolyte and film mercury electrode modified in situ by metallic ions can be used for detection of other drugs and DNA-intercalating dyes, as well as amino acids, peptides, and proteins determinations.…”

“…This finding has been attributed to indirect effects of HSV suppression on HIV replication. Spectroscopy 2 chromatographic, [3][4][5][6][7] chemiluminescence, 8,9 spectrophotometric, [10][11][12] and voltammetric [13][14][15][16][17][18] analytical methods have been developed for the determination of acyclovir. With recent advancements in properties of the adsorptive stripping voltammetry, new methodologies have been developed for adenine, thymine, guanine, Adenosine triphosphate (ATP), DNA, and antiretroviral drugs determinations, employing alkaline solution with lower ionic strength as the supporting electrolyte.…”

Stability‐indicating HPLC method for acyclovir and lidocaine in topical formulations

“…Several stability-indicating HPLC methods for the independent analysis of acyclovir (Bahrami, Mirzaeei, & Kiani, 2005;Brown, White, Chu, & Bartlett, 2002;Dao, Jiao, & Zhong, 2008;Fernández, Sepúlveda, Aránguiz, & von Plessing, 2003;Hasan, Chander, Ali, Baboota, & Ali, 2011;Huidobro, Ruperez, & Barbas, 2005;Stagni, Ali, & Weng, 2004;Tzanavaras & Themelis, 2007) or lidocaine (Gebauer, McClure, & Vlahakis, 2001;Malenovic, Medenica, Ivanovic, Jancic, & Markovic, 2005;Pendela, Kahsay, Baekelandt, Van Schepdael, & Adams, 2011;Plenis, Konieczna, Miękus, & Bączek, 2013;Prathyusha, Shanmugasundaram, & Naidu, 2013) in various formulations have been reported in the literature. To the best of our knowledge, no HPLC method has been reported for the simultaneous determination of acyclovir and lidocaine in combination formulations so far.…”

Quantification of acyclovir in dermal interstitial fluid and human serum by ultra‐high‐performance liquid–high‐resolution tandem mass spectrometry for topical bioequivalence evaluation

“…Acyclovir is a nucleoside analog and thus structurally similar to many endogenous substances; highly selective analytical methods are required for its detection in biofluids. Existing analytical methods for acyclovir are based on high-performance liquid chromatography (HPLC) with ultraviolet (Bahrami, Mirzaeei, & Kiani, 2005;Hasan, Chander, Ali, Baboota, & Ali, 2011) or fluorescence detection (Dao, Jiao, & Zhong, 2008), but these methods lack both selectivity and sensitivity. More recent methods that are based on HPLC-tandem mass spectrometry (HPLC-MS/MS) in combination with protein precipitation or solid-phase extraction (SPE) show improved sensitivity, which results in lower limits of quantification (LLOQ) (Holkar, Daphal, Yadav, & Rokade, 2012;Kanneti, Rajesh, Aravinda Raj, & Bhatt, 2009;Rigo-Bonnin et al, 2014;Shao, 2012).…”