A new view of insect–crustacean relationships II. Inferences from expressed sequence tags and comparisons with neural cladistics

Andrew, David R.

doi:10.1016/j.asd.2011.02.001

Cited by 60 publications

(62 citation statements)

References 77 publications

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“…LvGLUT1 is closely related to the crustacean D. pulex, and clustered with Hexapoda as Danaus plexippus. There are many phylogenetic studies where L. vannamei is clustered with insects and due to the absence of more malacostracan GLUT1 sequences available to form an special cluster; besides Tetraconata is one of the major groups of Arthropoda that includes the sister groups of Hexapoda and Crustacea [40], and since glucose transporters are very conserved proteins, as more crustacean sequences become available, their phylogeny will be better understood.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

The glucose transporter 1 -GLUT1- from the white shrimp Litopenaeus vannamei is up-regulated during hypoxia

et al. 2014

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During hypoxia the shrimp Litopenaeus vannamei accelerates anaerobic glycolysis to obtain energy; therefore, a correct supply of glucose to the cells is needed. Facilitated glucose transport across the cells is mediated by a group of membrane embedded integral proteins called GLUT; being GLUT1 the most ubiquitous form. In this work, we report the first cDNA nucleotide and deduced amino acid sequences of a glucose transporter 1 from L. vannamei. A 1619 bp sequence was obtained by RT-PCR and RACE approaches. The 5´ UTR is 161 bp and the poly A tail is exactly after the stop codon in the mRNA. The ORF is 1485 bp and codes for 485 amino acids. The deduced protein sequence has high identity to GLUT1 proteins from several species and contains all the main features of glucose transporter proteins, including twelve transmembrane domains, the conserved motives and amino acids involved in transport activity, ligands binding and membrane anchor. Therefore, we decided to name this sequence, glucose transporter 1 of L. vannamei (LvGLUT1). A partial gene sequence of 8.87 Kbp was also obtained; it contains the complete coding sequence divided in 10 exons. LvGlut1 expression was detected in hemocytes, hepatopancreas, intestine gills, muscle and pleopods. The higher relative expression was found in gills and the lower in hemocytes. This indicates that LvGlut1 is ubiquitously expressed but its levels are tissue-specific and upon short-term hypoxia, the GLUT1 transcripts increase 3.7-fold in hepatopancreas and gills. To our knowledge, this is the first evidence of expression of GLUT1 in crustaceans.

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Section: Phylogenetic Analysismentioning

confidence: 99%

The glucose transporter 1 -GLUT1- from the white shrimp Litopenaeus vannamei is up-regulated during hypoxia

et al. 2014

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“…Morphological and embryological evidence suggests a close affinity among Arthropoda, Onychophora, and Tardigrada (the Panarthropoda) (8,9), although the interrelationships among these three taxa are uncertain. Despite the concordance between these morphological studies and a few molecular analyses (10)(11)(12)(13)(14), most molecular studies instead support a close relationship between the water bears and the cycloneuralian ecdysozoans (nematodes, priapulids, and their close relatives), particularly the nematodes (2,(15)(16)(17)(18)(19)(20)(21)(22). These alternative hypotheses of tardigrade relationships have important consequences for our understanding of morphological evolution within Ecdysozoa.…”

mentioning

confidence: 90%

“…To better understand the nature of the signal in our EST dataset, we performed three experiments to test whether the Tardigrada + Nematoda group recovered in previous analyses (2,(15)(16)(17)(18)(19)(20)(21)(22) could result from a systematic error. First, Bayesian analyses were performed under a series of alternative models (Figs.…”

Section: Est-based Phylogenomic Analyses Support Panarthropoda Andmentioning

confidence: 99%

MicroRNAs and phylogenomics resolve the relationships of Tardigrada and suggest that velvet worms are the sister group of Arthropoda

Campbell

Rota‐Stabelli

Edgecombe

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

219

199

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Morphological data traditionally group Tardigrada (water bears), Onychophora (velvet worms), and Arthropoda (e.g., spiders, insects, and their allies) into a monophyletic group of invertebrates with walking appendages known as the Panarthropoda. However, molecular data generally do not support the inclusion of tardigrades within the Panarthropoda, but instead place them closer to Nematoda (roundworms). Here we present results from the analyses of two independent genomic datasets, expressed sequence tags (ESTs) and microRNAs (miRNAs), which congruently resolve the phylogenetic relationships of Tardigrada. Our EST analyses, based on 49,023 amino acid sites from 255 proteins, significantly support a monophyletic Panarthropoda including Tardigrada and suggest a sister group relationship between Arthropoda and Onychophora. Using careful experimental manipulations-comparisons of model fit, signal dissection, and taxonomic pruning-we show that support for a Tardigrada + Nematoda group derives from the phylogenetic artifact of longbranch attraction. Our small RNA libraries fully support our EST results; no miRNAs were found to link Tardigrada and Nematoda, whereas all panarthropods were found to share one unique miRNA (miR-276). In addition, Onychophora and Arthropoda were found to share a second miRNA (miR-305). Our study confirms the monophyly of the legged ecdysozoans, shows that past support for a Tardigrada + Nematoda group was due to long-branch attraction, and suggests that the velvet worms are the sister group to the arthropods.

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“…In this view, crustaceans are paraphyletic with respect to hexapods. Since interrelationships among the major crustacean subgroups are still controversial, the question which of them is the sister group of Hexapoda remains unanswered (Regier et al, 2008;von Reumont et al, 2009;Andrew, 2011). The position of Remipedia within Crustacea (or Pancrustacea) is also unclear (Boxshall, 2007;Richter et al, 2009;Jenner, 2010;Koenemann et al, 2010).…”

Section: Phylogenetic Considerationsmentioning

confidence: 96%

Ovary structure and early oogenesis in the remipede, Godzilliognomus frondosus (Crustacea, Remipedia): phylogenetic implications

Kubrakiewicz

Jaglarz

Iliffe

et al. 2012

Zoology

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A new view of insect–crustacean relationships II. Inferences from expressed sequence tags and comparisons with neural cladistics

Cited by 60 publications

References 77 publications

The glucose transporter 1 -GLUT1- from the white shrimp Litopenaeus vannamei is up-regulated during hypoxia

The glucose transporter 1 -GLUT1- from the white shrimp Litopenaeus vannamei is up-regulated during hypoxia

MicroRNAs and phylogenomics resolve the relationships of Tardigrada and suggest that velvet worms are the sister group of Arthropoda

Ovary structure and early oogenesis in the remipede, Godzilliognomus frondosus (Crustacea, Remipedia): phylogenetic implications

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