A New Yeast, <i>Malassezia yamatoensis</i>, Isolated from a Patient with Seborrheic Dermatitis, and Its Distribution in Patients and Healthy Subjects

Sugita, Takashi; Tajima, Mami; Takashima, Masako; Amaya, Misato; Saito, Masuyoshi; Tsuboi, Ryoji; Nishikawa, Akemi

doi:10.1111/j.1348-0421.2004.tb03554.x

Cited by 175 publications

(85 citation statements)

References 24 publications

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“…In 1996, in the reclassification by Gueho et (6,(9)(10)(11)(12)(13)(14). Among them, M. restricta and M. globosa are considered to be as the most important pathogenic organisms in the development of seborrheic dermatitis.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Malassezia Species in Patients with Pityriasis versicolor Compared with Healthy Individuals in Yazd, Iran

Jafari

Zarrinfar

Mirzaei

et al. 2013

Jundishapur J Microbiol

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Background: Malassezia species as skin microflora of humans and other warm-blooded vertebrates are the lipophilic yeasts associated with various human diseases, especially pityriasis versicolor (PV). Objectives: The objective of this study was to identify the Malassezia species of scraped skin of PV patients in Yazd, Iran, using morphological, biochemical and physiological methods. We also compared the obtained results of PV patients with normal healthy volunteers. Materials and Methods: A total of 200 persons, including 100 patients (with skin lesion) referred to Yazd Central Laboratory and 100 healthy volunteers as controls, were evaluated for Malassezia infection by morphological and biochemical methods. Results:The most commonly isolated species from PV lesions, were M. globosa (38.3%), M. furfur (29.4%), M. sympodialis (14.9%), M. pachydermatis (9.6%) and M. slooffiae (5.3%). Also the most commonly isolated species from healthy skins were M. furfur (37.2%), M. globosa (25.6%), M. sympodialis (16.3%), M. pachydermatis (13.9%) and M. slooffiae (4.6%). Totally M. globosa and M. furfur were the most frequented isolated. Conclusion: Highest prevalence of PV in our study was observed in the 20 -39 years old group, suggesting that the peak of the infection is coincided with ages and increasing sebum production in the highest level. M. globosa was the most commonly isolated Malassezia species of the patients group and M. furfur is the most common isolated species obtained from normal individuals skin samples. We couldn't find any significant differences between groups. The rate of isolated Malassezia species from patients was higher than normal individuals.

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Section: Introductionmentioning

confidence: 99%

Distribution of Malassezia Species in Patients with Pityriasis versicolor Compared with Healthy Individuals in Yazd, Iran

Jafari

Zarrinfar

Mirzaei

et al. 2013

Jundishapur J Microbiol

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“…The Malassezia species are considered part of the normal flora of the skin and are associated with pityriasis versicolor, Malassezia folliculitis, seborrheic dermatitis, dandruff, atopic dermatitis and psoriasis 1 [3][4][5][6][7] .…”

Section: Introductionmentioning

confidence: 99%

Comparison of Nested PCR and RFLP for Identification and Classification of Malassezia Yeasts from Healthy Human Skin

Song

Lee

et al. 2009

Ann Dermatol

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Background: Malassezia yeasts are normal flora of the skin found in 75∼98% of healthy subjects. The accurate identification of the Malassezia species is important for determining the pathogenesis of the Malassezia yeasts with regard to various skin diseases such as Malassezia folliculitis, seborrheic dermatitis, and atopic dermatitis. Objective: This research was conducted to determine a more accurate and rapid molecular test for the identification and classification of Malassezia yeasts. Methods: We compared the accuracy and efficacy of restriction fragment length polymorphism (RFLP) and the nested polymerase chain reaction (PCR) for the identification of Malassezia yeasts. Results: Although both methods demonstrated rapid and reliable results with regard to identification, the nested PCR method was faster. However, 7 different Malassezia species (1.2%) were identified by the nested PCR compared to the RFLP method. Conclusion: Our results show that RFLP method was relatively more accurate and reliable for the detection of various Malassezia species compared to the nested PCR. But, in the aspect of simplicity and time saving, the latter method has its own advantages. In addition, the 26S rDNA, which was targeted in this study, contains highly conserved base sequences and enough sequence variation for inter-species identification of Malassezia yeasts. (Ann Dermatol 21(4) 352

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“…Futhermore, recently three new species were included in this genus, namely M. dermatis, M. yamatoensis and M. nana (14,17,35,38). With the exception of M. pachidermatis, the remaining eight species have an absolute in vitro requirement for suplementation of long-chain fatty acids.…”

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Identification and pathogenicity of Malassezia species isolated from human healthy skin and with macules

Neves¹,

Magalhães²,

Silva³

et al. 2005

Braz. J. Microbiol.

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This study investigated the ocurrence of Malassezia species in clinically healthy students and with macules with a slight fawn discoloration and characterized the isolates as to the pathogenicity factors such as growth at 37ºC, lipase, phospholipase and protease detection. Clinical samples were collected from different body sites of one hundred students of different ages and both sexes. The samples, obtained by scrapping the skin surface and the scalp, were treated with potassa and cultured. Cultures were obtained in Petri dishes containing Sabouraud agar medium added of olive oil, incubated at room temperature and at 37ºC. Culture identifications were based in their morphological and physiological properties. Lipase, phospholipase and protease detection was performed in specific media on Petri dishes for formation of a zone. Globose, spherical yeast cells and hypha were investigated by direct microscopy of clinical materials. Malassezia furfur was detected in seven samples and M. sympodialis in four. All Malassezia cultures presented lipase activity, but none was phospholipase positive. Protease activity was observed in two M. furfur and two M. simpodialis isolates. Key words: Malassezia furfur, M. simpodialis, pathogenicityMalassezia is a lipophilic yeast frequently found in normal human skin. Current evidence indicates a high rate of skin colonization in healthy adults, in contrast with the low rate of colonization in prepubertal children (20).Gueho et al. (11) (14,17,35,38). With the exception of M. pachidermatis, the remaining eight species have an absolute in vitro requirement for suplementation of long-chain fatty acids. Based on studies conducted within the last 5 years, it appears that the most frequently organism associated with pityriasis versicolor may not be M. furfur and possible candidates include M. globosa (2,27) and M. sympodialis (15). The genus Malassezia was recently revised, but the clinical significance of each of these species is not clearly understood (15).Pityriasis versicolor (tinea versicolor) is a superficial fungal infection of the skin, appearing most commonly on the upper trunk, as well as on the upper arms, neck and face. As suggested by its name, pityriasis versicolor may be manifested in different colours, ranging from pink or to dark brown or even black. The lesions have a characteristic flaking appearance, although in larger lesions this can be evident only at the border of the macule, and can be hypopigmented or hyperpigmented. Generally, the lesions take the form of round or oval macules or papules, although in advanced cases the lesions may become confluent (13,21,33).

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A New Yeast, Malassezia yamatoensis, Isolated from a Patient with Seborrheic Dermatitis, and Its Distribution in Patients and Healthy Subjects

Cited by 175 publications

References 24 publications

Distribution of Malassezia Species in Patients with Pityriasis versicolor Compared with Healthy Individuals in Yazd, Iran

Distribution of Malassezia Species in Patients with Pityriasis versicolor Compared with Healthy Individuals in Yazd, Iran

Comparison of Nested PCR and RFLP for Identification and Classification of Malassezia Yeasts from Healthy Human Skin

Identification and pathogenicity of Malassezia species isolated from human healthy skin and with macules

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