A newly described strain of Eimeria arloingi (strain A) belongs to the phylogenetic group of ruminant-infecting pathogenic species, which replicate in host endothelial cells in vivo

Silva, Liliana; Chávez-Maya, Fernando; Macdonald, Sarah E.; Pegg, Elaine; Blake, Damer P.; Taubert, Anja; Hermosilla, Carlos

doi:10.1016/j.vetpar.2017.10.014

Cited by 16 publications

(10 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The closer the relationship of the host animals, the closer the relationship of Eimeria sp. parasitized on the host animals, which was confirmed by the phylogenetic tree (Silva et al, 2017;Zhao & Duszynski, 2001). The sequence data obtained in this study will help to understand the genetic diversity and geographical distribution of Eimeria species that infect ruminants worldwide.…”

Section: Discussionsupporting

confidence: 68%

Investigation and molecular identification of Eimeria sp. sampled from captive forest musk deer

Ren

Zhao

et al. 2021

PeerJ

View full text Add to dashboard Cite

Forest musk deer (Moschus berezovskii) is an endangered, protected species in China. Intestinal coccidiosis is a significant problem for captive forest musk deer. However, there are few reports on the prevalence and molecular characteristics of Eimeria sp. in forest musk deer. We sought to investigate the prevalence of Eimeria sp. in forest musk deer in the Sichuan and Shaanxi provinces in China. We also investigated the molecular characteristics of Eimeria sp. by analyzing the 18S rRNA gene. We collected a total of 328 fecal samples from forest musk deer on seven farms throughout the Sichuan and Shaanxi provinces. We extracted this parasite’s DNA and used this as a template for nested PCR amplification. The 18S rRNA gene fragment was associated with the plasmid vector, and these products were introduced into Escherichia coli (DH5α). The cultured bacterial solution was used as a PCR reaction template for identification purposes. We collected 328 fecal samples from forest musk deer in Lixian (n = 54), Maoxian (n = 52), Ma’erkang (n = 49), Dujiangyan (n = 55), Hanyuan (n = 41), Luding (n = 36) and Weinan (n = 41). One hundred ninety-eight (60.37%) fecal samples tested positive for Eimeria sp. . In our analysis of the 18S rRNA gene we found 34 types of Eimeria sp. with a similarity of 90.5–100%. We constructed a phylogenetic tree based on the parasite’s 18S rRNA gene sequence. Our findings indicated that the Eimeria sp. that parasitized the intestinal tract of forest musk deer was closely related to Eimeria alabamensis from Bos taurus and Eimeria ahsata from Ovis aries. To the best of our knowledge, ours was the first investigation and molecular identification of Eimeria sp. sampled from captive forest musk deer in China. Our results provide epidemiological data for the monitoring and prevention of Eimeria sp. in captive forest musk deer.

show abstract

Section: Discussionsupporting

confidence: 68%

Investigation and molecular identification of Eimeria sp. sampled from captive forest musk deer

Ren

Zhao

et al. 2021

PeerJ

View full text Add to dashboard Cite

show abstract

“…Combining with the phylogenetic tree, it showed that the closer the relationship of host animals was, the closer the relationship of Eimeria sp. parasitized on host animals was [51,52].…”

Section: Discussionmentioning

confidence: 99%

First report on molecular identification of Eimeria sp. from captive forest musk deer

Ren

Zhao

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Forest musk deer (Moschus berezovskii) is a national first-level protected and endangered wild animals in China. The intestinal coccidiosis of captive forest musk deer is one of the most important diseases. However, few studies have been conducted to quantify Eimeria sp. infection and to identify its molecules. Thus, the objective of this study was to investigate the Eimeria sp. infection in the intestinal tract of forest musk deer in Sichuan and Shaanxi, China, and to identify the 18S rRNA gene fragment of Eimeria sp. , which provides scientific basic experimental data for the molecular epidemiological investigation and population genetic analysis of Eimeria sp. of captive forest musk deer in 7 regions.Methods: 328 faecal samples of forest musk deer were collected from 7 farms. The DNA of Eimeria sp. in the positive samples was extracted and used as template for nested PCR amplification. The 18S rRNA gene fragment was connected with the plasmid vector, and the products were introduced into Escherichia coli (DH5α). The culture bacterial solution was used as a PCR reaction template for identification.Results: In total, we collected 328 faecal samples from forest musk deer in Lixian (n=54), Maoxian (n=52), Ma’erkang (n=49), Dujiangyan (n=55), Hanyuan (n=41), Luding (n=36) and Weinan (n=41). 198 (60.37%) faecal samples were positive for Eimeria sp. We analyzed the 18S rRNA gene sequence of Eimeria sp, and determined 34 types with similarity of 90.5% ~ 100%. A phylogenetic tree constructed based on the 18S rRNA gene sequence of Eimeria sp., it was confirmed that Eimeria sp. parasitized in the intestinal tract of forest musk deer was closely related to Eimeria alabamensis from Bos taurus, Eimeria faurei from Ovis aries and Eimeria ahsata from Ovis aries.Conclusions: To our knowledge, this was the first molecular identification of Eimeria sp. in the intestinal tract of forest musk deer. The study suggested that Eimeria sp. parasitizes in the intestinal tract of forest musk deer, which is their carrier.

show abstract

“…This disease is relevant to ruminant production; the economic impact was valued in 2012 as a 6-9% reduction in gross margin (Lassen and Østergaard 2012). The economic losses are a combination of costs, including prevention and treatment, and morbidity and mortality (Silva et al 2017;Squire et al 2019), resulting in adverse effects on feed intake, growth rate, fertility, wool growth and milk yield (Squire et al 2019;Silva et al 2020).…”

Section: Epidemiologymentioning

confidence: 99%

“…Ruminants become infested with the pathogens when they ingest the oocysts with contaminated feed or water (Constable et al 2016;Alcala-Canto et al 2020). The main source of sporulated oocysts is contamination of the environment in which ruminants are found, since oocysts can survive for weeks or months in favorable conditions of heat and humidity (Silva et al 2017;Keeton and Navarre 2018;Macrelli et al 2019;Trejo-Huitrón et al 2020;Gondipon and Malaka 2021). A large number of animals in a confined environment causes the environment to have a higher concentration of feces, which increases environmental contamination, although pathogens are also prolific in paddocks and feedlots, especially if water accumulates in nearby areas (Keeton and Navarre 2018;Macrelli et al 2019).…”

Section: Transmissionmentioning

confidence: 99%

Coccidiosis in Ruminants

2022

Int J Vet Sci

View full text Add to dashboard Cite

A newly described strain of Eimeria arloingi (strain A) belongs to the phylogenetic group of ruminant-infecting pathogenic species, which replicate in host endothelial cells in vivo

Cited by 16 publications

References 35 publications

Investigation and molecular identification of Eimeria sp. sampled from captive forest musk deer

Investigation and molecular identification of Eimeria sp. sampled from captive forest musk deer

First report on molecular identification of Eimeria sp. from captive forest musk deer

Coccidiosis in Ruminants

Contact Info

Product

Resources

About