A newly developed PCR assay ofH. pylori in gastric biopsy, saliva, and feces

Li, Chuanfu; Ha, Tuanzhu; Ferguson, Donald; David, S.; Zhao, Rongguo; Patel, N R; Krishnaswamy, Guha; Thomas, Eapen

doi:10.1007/bf02071393

Cited by 128 publications

(19 citation statements)

References 32 publications

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“…The PCR analysis on 102 human saliva samples revealed that 66 individuals were affected by H. pylori (Wnuk et al 2010). Of course, it should be further examined if genetic material of H. pylori is from live or dead bacteria, but the presence of live bacterial cells in saliva has been also documented by others (Li et al 1996). Thus, the presence of H. pylori in saliva may be important not only for the transmission of chronic infections of the gastrointestinal tract, but also for the propagation of chronic skin diseases in humans.…”

Section: H Pylori Infection and Systematic Diseasesmentioning

confidence: 97%

Helicobacter pylori-induced premature senescence of extragastric cells may contribute to chronic skin diseases

Lewińska

Wnuk

2017

Biogerontology

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Helicobacter pylori, one of the most frequently observed bacterium in the human intestinal flora, has been widely studied since Marshall and Warren documented a link between the presence of H. pylori in the gastrointestinal tract and gastritis and gastric ulcers. Interestingly, H. pylori has also been found in several other epithelial tissues, including the eyes, ears, nose and skin that may have direct or indirect effects on host physiology and may contribute to extragastric diseases, e.g. chronic skin diseases. More recently, it has been shown that H. pylori cytotoxin CagA expression induces cellular senescence of human gastric nonpolarized epithelial cells that may lead to gastrointestinal disorders and systemic inflammation. Here, we hypothesize that also chronic skin diseases may be promoted by stress-induced premature senescence (SIPS) of skin cells, namely fibroblasts and keratinocytes, stimulated with H. pylori cytotoxins. Future studies involving cell culture models and clinical specimens are needed to verify the involvement of H. pylori in SIPS-based chronic skin diseases.

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Section: H Pylori Infection and Systematic Diseasesmentioning

confidence: 97%

Helicobacter pylori-induced premature senescence of extragastric cells may contribute to chronic skin diseases

Lewińska

Wnuk

2017

Biogerontology

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“…It is a microaerophilic, Gram-negative bacterium characterized by a multisubunit urease that is crucial for its survival at acidic pH and successful colonization of the gastric environment [1]. In addition to culture, histology, urea breath test, or serology, infections with H. pylori can be detected by PCR in gastric biopsies [2,3].…”

Section: Introductionmentioning

confidence: 99%

Real-time quantitative PCR assay for the detection of Helicobacter pylori: no association with sudden infant death syndrome

et al. 2005

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Helicobacter pylori infection is known to be one of the most common chronic infectious diseases in humans. Recently, a hypothesis was proposed that H. pylori infection could be a frequent cause for sudden infant death syndrome (SIDS). We have investigated this postulated association by examining formalin-fixed paraffin-embedded gastric tissues of a retrospective cohort of 94 SIDS cases: The presence of H. pylori was inferred from a newly developed real-time quantitative PCR assay with SYBR Green I detection. This assay is based on the amplification of the single-copy H. pylori-specific glmM gene. Accuracy and precision were verified using a plasmid containing a 977-bp fragment of this glmM gene. The assay was very sensitive, and as few as 30 template copies per PCR reaction could be detected even in the presence of excess human DNA. The assay was validated on mucosal biopsy samples of patients with known H. pylori infections. Interfering effects due to SIDS gastric tissue were excluded. Only two (2.1%) of the SIDS samples yielded H. pylori DNA copy numbers and only beyond the lowest standard concentration. These results could be confirmed independently by immunohistochemistry using an H. pylori-specific antibody. Thus, an infection by H. pylori is very rare in cases of SIDS, and thus the postulated association of H. pylori infection and SIDS cannot be confirmed.

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“…[59] In fact, PCR of biopsy samples, saliva, and feces is widely used with sensitivity and specificity approaching 100%. [60] However, due to extensive polymorphism of H. pylori genes, the applicability of PCR is sometimes considered questionable. It has been revealed that H. pylori -specific region of the 16S ribosomal RNA sequence is unique for most H. pylori strains and therefore can be used for qualitative and quantitative assessment of the presence of H. pylori in biological specimens.…”

Section: Gastric Microbiomementioning

confidence: 99%

The Gastric and Intestinal Microbiome: Role of Proton Pump Inhibitors

Minalyan

Gabrielyan

Scott

et al. 2017

Curr Gastroenterol Rep

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Purpose of review The discovery of Helicobacter pylori and other organisms colonizing the stomach and the intestines has shed some light on the importance of microbiome in maintaining overall health and developing pathological conditions when alterations in biodiversity are present. The gastric acidity plays a crucial role in filtering out bacteria and preventing development of enteric infections. In this article, we discuss the physiology of gastric acid secretion and bacterial contribution to the composition of gastric and intestinal barriers and review the current literature on the role of proton pump inhibitors (PPIs) in the microbial biodiversity of the gastrointestinal tract. Recent findings Culture-independent techniques, such as 16S rRNA sequencing, have revolutionized our understanding of the microbial biodiversity in the gastrointestinal tract. Luminal and mucosa-associated microbial populations are not identical. Streptococcus is overrepresented in the biopsies of patients with antral gastritis and may also be responsible for the development of peptic ulcer disease. The use of PPIs favors relative streptococcal abundance irrespective of H. pylori status and may explain the persistence of dyspeptic symptoms in patients on PPI therapy. Increased risk of enteric infections has also been seen in patients taking PPIs. The overuse of PPIs leads to significant shift of the gastrointestinal microbiome towards a less healthy state. Summary With the advent of PPIs, many studies have demonstrated the significant changes in the microbial composition of both gastric and intestinal microbiota. Although they are considered relatively safe over-the-counter medications, PPIs in many cases are over- and even inappropriately used. Future studies assessing the safety of PPIs and their role in the development of microbiome changes should be encouraged.

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A newly developed PCR assay ofH. pylori in gastric biopsy, saliva, and feces

Cited by 128 publications

References 32 publications

Helicobacter pylori-induced premature senescence of extragastric cells may contribute to chronic skin diseases

Helicobacter pylori-induced premature senescence of extragastric cells may contribute to chronic skin diseases

Real-time quantitative PCR assay for the detection of Helicobacter pylori: no association with sudden infant death syndrome

The Gastric and Intestinal Microbiome: Role of Proton Pump Inhibitors

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