A newly isolated<i>Enterobacter</i>sp. strain produces 2,3-butanediol during its cultivation on low-cost carbohydrate-based substrates

Palaiogeorgou, Anastasia Marina; Papanikolaou, Séraphim; Castro, Aline Machado de; Freire, Denise Maria Guimarães; Kookos, Ioannis K.; Koutinas, Apostolis

doi:10.1093/femsle/fny280

Cited by 18 publications

(10 citation statements)

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“…Furthermore, in the experiment with the somehow lower Gly 0 concentration added into the medium (Gly 0 ≈ 70 g/L), when the glycerol concentration from the medium was exhausted, polyols re-consumption and new yeast cell biomass occurred (Table 1). Re-consumption of extra-cellular and previously synthesized polyols in favor of the biosynthesis of new cell material (or potentially the production of other metabolites, like citric acid) has been observed in batch cultures performed by various wild-type Y. lipolytica strains [20][21][22]35,38]. Moreover, as stated, when higher Gly 0 concentrations were employed, in absolute values (viz.…”

Section: Metabolic Characterization Of Yarrowia Lipolytica Lmbf Y-46:...mentioning

confidence: 81%

“…In the case of the shake-flask cultures and for various fermentation points, the DOT was determined according to the procedure proposed in Palaiogeorgou et al [20]. In all of the cases in which DOT was measured (measurements were conducted at the phase in which polyols were accumulated into the medium), and for all of the Gly 0 quantities set into the medium, DOT values were between 20% and 50% v/v.…”

Section: Analysesmentioning

confidence: 99%

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Physiological Characterization of a Novel Wild-Type Yarrowia lipolytica Strain Grown on Glycerol: Effects of Cultivation Conditions and Mode on Polyols and Citric Acid Production

et al. 2020

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A new yeast wild-type Yarrowia lipolytica isolate presented efficient growth on glycerol. During flask cultures, nitrogen limitation led to the secretion of sugar-alcohols as the major metabolites of the process (mannitol, arabitol and erythritol), whereas insignificant quantities of citrate were synthesized. Although in some instances high initial glycerol concentrations were employed (≈150 g/L), remarkable glycerol assimilation and polyol secretion was observed. Total polyols ≈ 52 g/L (conversion yield on glycerol consumed = 0.43 g/g) was recorded in the flask experiments. The sugar-alcohol production bioprocess was successfully simulated with the aid of a modified Velhlust–Aggelis model that fitted very well with the experimental data, while optimized parameter values seemed to be quite consistent. In bioreactor trials, a noticeable metabolic shift towards citric acid production was observed, while simultaneously insignificant polyol quantities were produced. In fed-batch bioreactor experiments, a total citric acid quantity ≈ 102 g/L was recorded—one of the highest in the literature for wild-type Y. lipolytica strains. This metabolic transition was due to higher oxygen saturation into the medium that occurred in the bioreactor experiments compared with the flasks. Cellular lipids produced in the bioreactor trial contained higher concentrations of unsaturated fatty acids compared with those produced in flasks.

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Section: Metabolic Characterization Of Yarrowia Lipolytica Lmbf Y-46:...mentioning

confidence: 81%

Section: Analysesmentioning

confidence: 99%

Physiological Characterization of a Novel Wild-Type Yarrowia lipolytica Strain Grown on Glycerol: Effects of Cultivation Conditions and Mode on Polyols and Citric Acid Production

et al. 2020

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“…In the 250 mL shake-flasks, dissolved oxygen tension (DOT, in % v/v) was determined off Line using a selective electrode (OXI 96, B-SET, Weilheim, Germany) [49]. Before harvesting, the shaker was stopped and the probe was placed into the flask, after which the shaker was again switched on and the measurement was taken after DOT equilibration (this usually happened within the next 10 min).…”

Section: Methodsmentioning

confidence: 99%

“…Oxygen saturation into the medium (DOT, % v/v) was measured in both the 250 mL shake-flask experiments performed, and, in both instances, DOT values for all fermentation points were >25% v/v (Figure 4). It can thus be assumed, that cultures in such types of batch experiment (trials in 250 mL flasks filled with 50 mL of medium) were carried out under fully aerobic conditions [49,[54][55][56]. Moreover, as was anticipated [46,48], significant quantities of ethanol were accumulated into the culture medium, since S. cerevisiae is one of the most typical "Crabtree-positive" yeast species known to exist in the literature [5,57].…”

Section: Medium Type Time (Hoursmentioning

confidence: 99%

Effect of Myclobutanil Pesticide on the Physiological Behavior of Two Newly Isolated Saccharomyces cerevisiae Strains during Very-High-Gravity Alcoholic Fermentation

et al. 2019

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Yeasts are able to act as biosorbents, as their cell wall includes several components capable of binding organic xenobiotic compounds that can potentially be removed during various fermentation processes. In the present investigation, two novel Saccharomyces cerevisiae strains (LMBF-Y 16 and LMBF-Y-18), previously isolated from grapes, were studied regarding their physiological behavior (dry cell weight—DCW production, substrate uptake, and ethanol and glycerol biosynthesis) during fermentations of grape must, in some cases enriched with commercial glucose and fructose (initial total sugar concentration approximately 150 and 250 g/L, respectively). Myclobutanil (a chiral triazole fungicide broadly used as a protective agent of vine) was also added to the culture media at various concentrations in order to assess the ability of the yeasts to simultaneously perform alcoholic fermentations and detoxify the medium (i.e., to remove the fungicide). In the first set of experiments and for both tested strains, trials were carried out in either 250 mL or 2.0 L agitated shake flasks in either synthetic glucose-based experiments or grape musts. Since the results obtained in the trials where the cultures were placed in 2.0 L flasks with grape musts as substrates were superior in terms of both DCW and ethanol production, these experimental conditions were selected for the subsequent studies. Both strains showed high fermentative efficiency, producing high amounts of DCW (9.5–10.5 g/L) in parallel with high ethanol production, which in some cases achieved values very close to the maximum theoretical ethanol production yield (≈0.49 g of ethanol per g of sugar). When using grape must with initial total sugars at approximately 250 g/L (very high gravity fermentation media, close to winemaking conditions), significantly high ethanol quantities (i.e., ranging between 105 and 123 g/L) were produced. Myclobutanil addition slightly negatively affected sugar conversion into ethanol; however, in all cases, ethanol production was very satisfactory. A non-negligible myclobutanil removal during fermentation, which ranged between 5%–27%, as a result of the adsorptive or degradative capacity of the yeast was also reported. The presence of myclobutanil had no effect on DCW production and resulted in no significant differences in the biosynthesis of glycerol. Therefore, these newly isolated yeast strains could be excellent candidates for simultaneous high ethanol production and parallel pesticide removal in a general biorefinery concept demonstrating many environmental benefits.

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“…The metabolic pathways involved in glycerol assimilation can lead to a plethora of products (e.g., PDO, BDO, EtOH, citric acid, lactic acid, polyols, etc. ), depending on the microorganism and the fermentation conditions employed [ 6 , 7 , 8 , 9 , 10 ]. Moreover, biodiesel-derived glycerol can be biotechnologically converted through a one-step process into fermentation products using microorganisms such as bacteria, fungi and yeasts [ 5 , 11 ], but also into even more customized added-value molecules, when chemical or enzymatic processes follow.…”

Section: Introductionmentioning

confidence: 99%

Screening of New Industrially Important Bacterial Strains for 1,3-Propanediol, 2,3-Butanediol and Ethanol Production through Biodiesel-Derived Glycerol Fermentations

et al. 2023

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A study on the ability of new microbial strains to assimilate biodiesel-derived glycerol at low purity (75% w/w) and produce extra-cellular platform chemical compounds of major interest was carried out. After screening several bacterial strains under different fermentation conditions (e.g., pH, O2 availability, glycerol purity), three of the screened strains stood out for their high potential to produce valued-added products such as 2,3-butanediol (BDO), 1,3-propanediol (PDO) and ethanol (EtOH). The results indicate that under aerobic conditions, Klebsiella oxytoca ACA-DC 1581 produced BDO in high yield (YBDO/Gly = 0.46 g/g, corresponding to 94% of the maximum theoretical yield; Ymt) and titer, while under anaerobic conditions, Citrobacter freundii NRRL-B 2645 and Enterobacter ludwigii FMCC-204 produced PDO (YPDO/Gly = 0.56 g/g, 93% of Ymt) and EtOH (YEtOH/Gly = 0.44 g/g, 88% of Ymt), respectively. In the case of C. freundii, the regulation of pH proved to be mandatory, due to lactic acid production and a subsequent drop of pH that resulted in fermentation ceasing. In the fed-batch culture of K. oxytoca, the BDO maximum titer reached almost 70 g/L, the YBDO/Gly and the mean productivity value (PrBDO) were 0.47 g/g and 0.4 g/L/h, respectively, while no optimization was imposed. The final BDO production obtained by this wild strain (K. oxytoca) is among the highest in the international literature, although the bioprocess requires optimization in terms of productivity and total cost. In addition, for the first time in the literature, a strain from the species Hafnia alvei (viz., Hafnia alvei ACA-DC 1596) was reported as a potential BDO producer. The strains as well as the methodology proposed in this study can contribute to the development of a biorefinery that complements the manufacture of biofuels with high-value biobased chemicals.

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A newly isolatedEnterobactersp. strain produces 2,3-butanediol during its cultivation on low-cost carbohydrate-based substrates

Cited by 18 publications

References 50 publications

Physiological Characterization of a Novel Wild-Type Yarrowia lipolytica Strain Grown on Glycerol: Effects of Cultivation Conditions and Mode on Polyols and Citric Acid Production

Physiological Characterization of a Novel Wild-Type Yarrowia lipolytica Strain Grown on Glycerol: Effects of Cultivation Conditions and Mode on Polyols and Citric Acid Production

Effect of Myclobutanil Pesticide on the Physiological Behavior of Two Newly Isolated Saccharomyces cerevisiae Strains during Very-High-Gravity Alcoholic Fermentation

Screening of New Industrially Important Bacterial Strains for 1,3-Propanediol, 2,3-Butanediol and Ethanol Production through Biodiesel-Derived Glycerol Fermentations

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