A NIR luminescent copolymer based on platinum porphyrin as high permeable dissolved oxygen sensor for microbioreactors

Abstract

in Wiley Online Library (wileyonlinelibrary.com) Microbioreactors with multioptical sensors have become increasingly important because of their small working volumes, high degree of parallelization and the available robotics. A novel hydrophobic luminescent copolymer P(Pt-TPP-TFEMA) along with reference P(Pt-TPP-EMA) containing the pendant group of 5,10,15,20-tetraphenylporphyrin (TPP) moiety as low-cost dissolved oxygen (DO) chemosensor film for high-throughput microbioreactors is designed. Its sensor film exhibits fast response to DO with good stability and fatigue resistance, being capable of applying as a low-cost DO indicator for high-throughput bioprocess measurement. Results show that the quenching response of DO increases with the enhancement in the copolymeric hydrophobicity using the presented hybrid fluorinated ethyl methacrylate. Furthermore, the long emission band at 650 nm of chromophore TPP with large Stoke's shift about 250 nm brings several advantages, such as low scattering, deep penetration, and minimal interferences of absorption and fluorescence from the fermentation system, which shows high-promising application in bioprocess monitoring.

Review of Dissolved Oxygen Detection Technology: From Laboratory Analysis to Online Intelligent Detection

“…Such fluorescence-sensitive substances do not consume dissolved oxygen, have a high response rate (less than 50 ms), and are relatively stable. To improve the detection sensitivity, ruthenium–chromium complexes, as well as platinum phosphor porphyrins such as platinum tetrafluorophenyl porphyrins (PtTFPP) and platinum octaethyl porphyrins (PtOEP), are also widely used as fluorescence indicators in optical oxygen sensors to detect the concentration of dissolved oxygen [108,109]. The fluorescence intensity of these types of metal compounds corresponds to the dissolved oxygen partial pressure, and the atomic excited state of these metal compounds has a long life and is very stable, making these compounds ideal fluorescence sensitivity indicators.…”

Integration and application of optical chemical sensors in microbioreactors

“…Many papers have described the use of oxygen sensors in lab-on-a-chip or lab-on-a-disk technology, showing a variety of different dyes and polymeric matrices being used for this purpose. In 2013, Jin et al 68 presented a platinum porphyrin-based, NIR luminescent copolymer for the measurement of dissolved oxygen in microbioreactors, these were covalently bonded to the polymer. Sensor materials, with absorption/emission profiles in the red or near-infrared, are beneficial because this will reduce scattering and background signal due to the low absorption and auto-fluorescence of biomolecules in the NIR spectral region.…”

Rapid prototyping of a novel and flexible paper based oxygen sensing patchviaadditive inkjet printing process

“…The optical probes are used to excite the dyes using UV/visible light and the emitted uorescence is quenched in proportion to the oxygen concentration in the surrounding environment. [9][10][11] Currently, a common method to fabricate the oxygen sensitive patches are by immobilizing uorescent dyes in polymers or sol-gel matrix. Then, conventional ink deposition processes such as dip coating or spin coating are employed to deposit the sol-gel matrix with immobilized uorescent dyes on rigid, transparent and porous glass bead structures.…”

“…Then, conventional ink deposition processes such as dip coating or spin coating are employed to deposit the sol-gel matrix with immobilized uorescent dyes on rigid, transparent and porous glass bead structures. [10][11][12][13] Another method that has been used to fabricate oxygen sensitive patches are by embedding uorescent dyes in silicone and then subjecting it to thermal annealing, under a nitrogen ux at high temperatures. 14 These methods are relatively costly as well as complex and are not suitable for rapid prototyping and mass production.…”