A nitrite biosensor based on the immobilization of Cytochrome c on multi-walled carbon nanotubes–PAMAM–chitosan nanocomposite modified glass carbon electrode

Chen, Quanpeng; Ai, Shiyun; Zhu, Xiangbin; Yin, Huanshun; Ma, Qiang; Qiu, Yanyan

doi:10.1016/j.bios.2009.03.007

Cited by 95 publications

(45 citation statements)

References 43 publications

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“…Therefore, the sBLM thickness is comparable with the thickness of the protein layer (approx. 3 nm [28]). That is why the capacitance responds with an exceptional sensitivity to minute amount of adsorbed cyt c.…”

Section: Resultsmentioning

confidence: 97%

Electrochemical Impedance Spectroscopy for Assessing the Recognition of Cytochrome c by Immobilized Calixarenes

et al. 2011

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We developed a biosensor based on supported lipid films (sBLM) with incorporated calixarenes for detection of cytochrome c (cyt c) using electrochemical impedance spectroscopy (EIS). Calix[6]arene (CALIX) was incorporated into soybean phosphatidylcholine (SBPC) liposomes in different molar ratios (SBPC:CALIX = 10 : 1, 30 : 1 and 100 : 1). The lipid layer containing calixarenes has been formed on the surface of a gold electrode previously covered with an octadecanethiol monolayer. Both the sBLM capacitance and the electron transfer resistance showed noticeable modification at a cyt c concentration as low as 10 nM. However, a much higher concentration of free lysine (> 30 mM) was necessary to induce a similar effect. Nonspecific interaction of cyt c with sBLM without CALIX was negligible.

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Section: Resultsmentioning

confidence: 97%

Electrochemical Impedance Spectroscopy for Assessing the Recognition of Cytochrome c by Immobilized Calixarenes

et al. 2011

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“…For example, Ultraviolet Visible Spectroscopy (UVevis) spectra of the CNP can be obtained before and after protein conjugation [62,63,68]. Characteristic spectroscopic peaks of a desired functional group will appear after conjugation, confirming their successful incorporation onto the CNP surface.…”

Section: Characterization Of Protein-carbon Nanoparticle Conjugatesmentioning

confidence: 94%

Protein functionalized carbon nanomaterials for biomedical applications

Oliveira

Bisker

Bakh

et al. 2015

Carbon

192

111

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a b s t r a c tSince the discovery of low-dimensional carbon allotropes, there is increasing interest in using carbon nanomaterials for biomedical applications. Carbon nanomaterials have been utilized in the biomedical field for bioimaging, chemical sensing, targeting, delivery, therapeutics, catalysis, and energy harvesting. Each application requires tailored surface functionalization in order to take advantage of a desired property of the nanoparticles. Herein, we review the surface immobilization of bio-molecules, including proteins, peptides, and enzymes, and present the recent advances in synthesis and applications of these conjugates. The carbon scaffold and the biological moiety form a complex interface which presents a challenge for achieving efficient and robust binding while preserving biological activity. Moreover, some applications require the utilization of the protein-nanocarbon system in a complex environment that may hinder its performance or activity. We analyze different strategies to overcome these challenges when using carbon nanomaterials as protein carriers, explore various immobilization techniques along with characterization methods, and present recent demonstrations of employing these systems for biomedical applications. Finally, we consider the challenges and future directions of this field.

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“…Estos estudios han analizado la interacción de los polímeros durante la formación de las películas, así como el efecto de la distribución e interacciones de los compuestos sobre las propiedades físicas, antimicrobianas, químicas, entre otras [59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74]. Muchas aplicaciones han sido desarrolladas en campos de la investigación como en el área de electrónica [75][76][77][78][79][80][81][82][83][84][85], la inmovilización de enzimas [86][87][88][89], el desarrollo de nuevos materiales con propiedades ópticas específicas [81,[90][91][92], la liberación de compuestos [93][94][95], entre otras.…”

Section: Conformación De Películas En Multicapasunclassified

Modificaciones físicas, químicas y enzimáticas y sus efectos sobre las propiedades de las películas de quitosano

Valderrama

Albarracín

2014

Matéria (Rio J.)

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Modificaciones físicas, químicas y enzimáticas y sus efectos sobre las propiedades de las películas de quitosano.Physical, chemical and enzymatic modifications and their effects on the properties of chitosan films. RESUMENLas recientes investigaciones en el campo de biopolímeros han intentado brindar soluciones tecnológicas a los retos en el campo de la ciencia y la tecnología de los materiales. En los últimos años, se han llevado a cabo investigaciones con el objetivo de modificar las propiedades de películas empleando tratamientos enzimáticos y físicos, tales como la aplicación de radiaciones, pulsos eléctricos, plasma, tratamientos térmicos, uso de fluidos supercríticos, así como la conformación de películas en multicapas. Estas modificaciones cambian las propiedades antimicrobianas, físicas y químicas de las películas de quitosano y permiten desarrollar una extensa cantidad de aplicaciones en los campos de biomedicina, farmacología, biotecnología y en la industria de cosméticos y de alimentos. El objetivo del presente artículo de revisión es dar a conocer los nuevos avances en el campo de modificaciones físicas y enzimáticas de películas de quitosano explicando diferentes técnicas empleadas en los últimos años.Palabras clave: quitosano, polímeros, películas modificadas, aditivos alimenticios. ABSTRACTRecent research on biopolymer has focused its efforts to provide technological solutions to current and future challenges in the field of science and technology of materials. The properties of chitosan films have been modified by enzymatic and physical treatments such as the application of radiation, electric pulses, plasma, heat treatments, use of supercritical fluids as well as the formation of multilayer films. These modifications change antimicrobial, physical and chemical properties of chitosan films, and allow developing a wide range of applications in the fields of biomedicine, pharmacology, biotechnology, cosmetic and food industries. The purpose of this review is to present new developments in the field of physical and enzymatic modifications of chitosan films describing the different techniques carried out on the last years.

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A nitrite biosensor based on the immobilization of Cytochrome c on multi-walled carbon nanotubes–PAMAM–chitosan nanocomposite modified glass carbon electrode

Cited by 95 publications

References 43 publications

Electrochemical Impedance Spectroscopy for Assessing the Recognition of Cytochrome c by Immobilized Calixarenes

Electrochemical Impedance Spectroscopy for Assessing the Recognition of Cytochrome c by Immobilized Calixarenes

Protein functionalized carbon nanomaterials for biomedical applications

Modificaciones físicas, químicas y enzimáticas y sus efectos sobre las propiedades de las películas de quitosano

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