A Non-Biological Method for Screening Active Components against Influenza Virus from Traditional Chinese Medicine by Coupling a LC Column with Oseltamivir Molecularly Imprinted Polymers

Yang, Yajun; Li, Jianyong; Liu, Xi-Wang; Zhang, Jiyu; Liu, Yurong; Li, Bing

doi:10.1371/journal.pone.0084458

Cited by 11 publications

(11 citation statements)

References 34 publications

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“…Adsorption properties of the OSMIP@silica gel were evaluated by static adsorption experiments. The chromatographic performance of the OSMIP@silica gel has been improved significantly compared to our previous reports [ 8 , 9 ]. The column had good affinity and selectivity for OS and peramivir, which is a neuraminidase inhibitor too.…”

Section: Introductionmentioning

confidence: 73%

“…Nowadays, MIPs are used for screening analogues with similar bioactivities to search for new drug candidates from plant extracts [ 5 , 6 ] or combinatorial chemistry libraries [ 7 ]. The aim of our serial studies on MIP was to develop a nonbiological method for screening active components from traditional Chinese medicine (TCM) by coupling a liquid-chromatography (LC) column with MIPs [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…In our previous studies [ 8 , 9 ], the influenza virus neuraminidase inhibitor oseltamivir (OS) was used as template to synthesize an oseltamivir molecularly imprinted polymer (OSMIP) through bulk polymerization. The bulk polymers were grinded, sieved and packed into a stainless-steel LC column (150 mm × 4.6 mm, 45–60 μm).…”

Section: Introductionmentioning

confidence: 99%

“…MeOH–MeCN–formic acid (75:25:0.01) was used as the mobile phase at the flow rate of 0.075 mL·min −1 to ensure the system pressure was lower than the packing pressure of 2000 psi. Therefore, the peak widths of OS and other analytes were very wide and the analytical time of every sample was longer than 5 h [ 8 , 9 ]. On account of this, one of the main objectives of this study was to improve the chromatographic performance of the OSMIP LC column and shorten the analytical time of the samples.…”

Section: Introductionmentioning

confidence: 99%

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Preparation and Evaluation of Oseltamivir Molecularly Imprinted Polymer Silica Gel as Liquid Chromatography Stationary Phase

et al. 2018

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To improve the chromatographic performance of an oseltamivir (OS) molecularly imprinted polymer (MIP), silica gel coated with an MIP layer for OS (OSMIP@silica gel) was prepared by the surface molecular imprinting technology on the supporter of porous silica gel microspheres. A nonimprinted polymer with the silica gel (NIP@silica gel) was also prepared for comparison. The obtained particles were characterized through FT–IR, scanning electron microscopy, specific surface area analysis, and porosity measurements. The results indicated that the polymer was successfully synthesized and revealed the structural differences between imprinted and nonimprinted polymers. The results of static adsorption experiments showed that adsorption quantity of the OSMIP@silica gel for OS was higher than that for NIP@silica gel, and the OSMIP@silica gel had two kinds of affinity sites for OS but the NIP@silica gel had one. The chromatographic performance of the OSMIP@silica gel column had significant improvement. The imprinting factor of the OSMIP@silica gel column for OS was 1.64. Furthermore, the OSMIP@silica gel column showed good affinity and selectivity for template OS and another neuraminidase inhibitor, peramivir, but not for quinocetone. These results indicated that the prepared OSMIP could be used to simulate the activity center of neuraminidase, and the OSMIP@silica gel column could be also employed in future studies to search for more active neuraminidase inhibitor analogues from traditional Chinese herbs.

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Section: Introductionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Preparation and Evaluation of Oseltamivir Molecularly Imprinted Polymer Silica Gel as Liquid Chromatography Stationary Phase

et al. 2018

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“…Some studies are used for qualitation [25, 26]. Compared with LC-Q-MS, LC-QQQ-MS can select particular ions to collide and analyze the fragments after collision.…”

Section: Lc-msmentioning

confidence: 99%

The Applications and Features of Liquid Chromatography‐Mass Spectrometry in the Analysis of Traditional Chinese Medicine

Pang

Zhu

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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With increasingly improved separation of complex samples and detection of unknown material capabilities, liquid chromatography coupled with mass spectrometry (LC-MS) has been widely used in traditional Chinese medicine (TCM) research. This article describes the principles of liquid chromatography (LC) and mass spectrometry (MS) and their advantages and disadvantages in qualitative and quantitative analysis of TCM. We retrieved research literatures about the application of LC-MS in TCM published during the past five years at home and abroad. To better guide the analysis of TCM, this review mainly focuses on the applications category of LC-MS, how often different kinds of LC-MS are used, and the qualitative and quantitative ability of various LC-MS in the study of TCM.

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Efficient discovery and capture of new neuronal nitric oxide synthase–postsynaptic density protein‐95 uncouplers from herbal medicines using magnetic molecularly imprinted polymers as artificial antibodies

Huang

Zhang

et al. 2017

J of Separation Science

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In the scope of stroke treatment, new neuronal nitric oxide synthase-postsynaptic density protein-95 uncouplers from herbal medicines were discovered and captured. To do so, highly selective magnetic molecularly imprinted polymers with a core-shell structure were prepared as artificial antibodies. According to the results of computational simulations, we designed and synthesized various polymers with varying amounts and types of template, functional monomer, cross-linker, and solvent. Characterization and performance tests revealed that the most appropriate artificial antibodies showed uniform spherical morphologies, large adsorption capacities, fast-binding kinetics, high selectivity, and quick separation. These artificial antibodies were then used as sorbents for dispersive magnetic solid-phase extraction coupled with high-performance liquid chromatography and mass spectrometry to capture and identify structural analogs to ZL006 from extracts of Scutellariae radix, Psoraleae fructus, and Trifolium pratense. Furthermore, according to the neuroprotective effect and coimmunoprecipitation test, Baicalein, Neobavaisoflavone, Corylifol A, and Biochanin A can be the potential uncouplers of neuronal nitric oxide synthase-postsynaptic density protein-95. Therefore, this present study contributes valuable information for the discovery of neuronal nitric oxide synthase-postsynaptic density protein-95 uncouplers from herbal medicines.

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A Non-Biological Method for Screening Active Components against Influenza Virus from Traditional Chinese Medicine by Coupling a LC Column with Oseltamivir Molecularly Imprinted Polymers

Cited by 11 publications

References 34 publications

Preparation and Evaluation of Oseltamivir Molecularly Imprinted Polymer Silica Gel as Liquid Chromatography Stationary Phase

Preparation and Evaluation of Oseltamivir Molecularly Imprinted Polymer Silica Gel as Liquid Chromatography Stationary Phase

The Applications and Features of Liquid Chromatography‐Mass Spectrometry in the Analysis of Traditional Chinese Medicine

Efficient discovery and capture of new neuronal nitric oxide synthase–postsynaptic density protein‐95 uncouplers from herbal medicines using magnetic molecularly imprinted polymers as artificial antibodies

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