A Non-Enzymatic Electrochemical Sensing Platform Based on Hemin@MOF Composites for Detecting Hydrogen Peroxide and DNA

Abstract

In this work, Hemin@MOF was prepared by doping hemin into Cu-MOF-199 and applied in the electrochemical sensing of H 2 O 2 and DNA. The Hemin@MOF modified electrode (Hemin@MOF/GCE) exhibited an excellent electrocatalytic activity toward H 2 O 2 and the detection limit of H 2 O 2 was as low as 0.07 μmol • L −1 with the range of 0.1 to 2200 μmol • L −1 . Based on the eminent electrochemical performance, Hemin@MOF was further employed as an electrochemical signal indicator to construct a novel electrochemical DNA biosensor. Here, via amidation reaction, the stem-loop-structure probe DNA (pDNA) carrying 3'-amino and 5'-biotin was anchored on the carboxyl functionalized graphene. Then the pDNA was unfolded by the hybridization of the matched target DNA (tDNA). The following recognition between streptavidin (SA)

Nonenzymatic Glucose Sensing and Magnetic Property Based On the Composite Formed by Encapsulating Ag Nanoparticles in Cluster-Based Co-MOF

“…MOFs have been discovered in a wide range of applications. − Especially, the redox active MOFs have been designed and showed a considerable potential for electrochemical energy storage and electrochemical sensing . For example, Cheng et al reported a Hemin@MOF composite, which as a nonenzymatic electrochemical sensor can detect H 2 O 2 and DNA with a low detection limit (6.9 × 10 –16 mol L –1 ) . Long et al developed a feasible method to prepare MOF-template porous material with CuCo oxide arrays, providing a way to improve the performance of nonenzymatic glucose sensor …”

Improved Hydrogen Sensitivity and Selectivity in PdO with Metal-Organic Framework Membrane

“…However, these membranes do not protect against other small molecules, and both sensing platforms use much higher power than the CS-FET platform. 8 MOFs have also been used as part of a composite with carbon-based sensing materials, [28][29][30][31] especially for adsorption of biologically relevant molecules, but these systems are still mostly leveraging the size and chemistry of the MOF and not the potential electronic effect the MOF can have on the support. Using a support to affect the electronic structure of the sensing layer, as we show here, is another tool to improve selectivity.…”

Nanomaterials for molecular signal amplification in electrochemical nucleic acid biosensing: recent advances and future prospects for point-of-care diagnostics

“…There have been several reports demonstrating the peroxidase-mimicking properties of MOFs functionalized with streptavidin for DNA detection, either as an indirect label that binds to a streptavidin aptamer when target DNA is absent, 75 or as a direct label, binding the biotinylated complementary DNA strand. 76,77 Recent advances in catalysis and magnetic separation of MOFs, suggest that such materials hold significant promise for electrochemical signal amplification. 78,79 Sandwich type assays, where the target is immobilized between a capture and label probe, represent the most common approach for nanoparticle-based nucleic acid sensors.…”