A non-equilibrium state of deoxyhaemoglobin

Leeuwen, Johan W. van; Butler, J. A. V.; Swallow, A. J.

doi:10.1016/0005-2795(81)90079-9

Cited by 5 publications

(1 citation statement)

References 31 publications

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“…For example, the Arrhenius activation energy for autoxidation of CYP3A4 saturated with the substrate testosterone is 22 kcal/mol (48), and for autoxidation of CYP19 with androstenedione, it is 18 kcal/mol (51). Contrary, the O 2 and CO binding rates have significantly lower activation energies in the range 6 – 9 kcal/mol, as documented for several heme proteins (52-55). Hence, the ratio k binding /k autox increases with decreasing temperature.…”

Section: Introductionmentioning

confidence: 65%

Cryoradiolysis and Cryospectroscopy for Studies of Heme-Oxygen Intermediates in Cytochromes P450

Denisov

Grinkova

Sligar

2012

Methods in Molecular Biology

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Cryogenic radiolytic reduction is one of the most simple and convenient methods of generation and stabilization of reactive iron-oxygen intermediates for mechanistic studies in chemistry and biochemistry. The method is based on one-electron reduction of the precursor complex in frozen solution via exposure to the ionizing radiation at cryogenic temperatures. Such approach allows for accumulation of the fleeting reactive complexes which otherwise could not be generated at sufficient amount for structural and mechanistic studies. Application of this method allowed for characterizing of peroxoferric and hydroperoxo-ferric intermediates, which are common for the oxygen activation mechanism in cytochromes P450, heme oxygenases and nitric oxide synthases, as well as for the peroxide metabolism by peroxidases and catalases.

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