A Non-invasive Imageable GFP-expressing Mouse Model of Orthotopic Human Bladder Cancer

Abstract

Background/Aim: A more realistic mouse model of bladder cancer is necessary to develop effective drugs for the disease. Tumor models enhanced by bright fluorescent-reporter genes to follow the disease in real-time would enhance the ability to accurately predict the efficacy of various therapeutics on this particularly-malignant human cancer. Materials and Methods: A highly-fluorescent green fluorescent protein (GFP)expressing bladder cancer model was orthotopically established in nude mice using the UM-UC-3 human bladder-cancer cell line (UM-UC-3-GFP). Fragments from a subcutaneous tumor of UM-UC-3-GFP were surgically implanted into the nude mouse bladder. Non-invasive and intra-vital fluorescence imaging was obtained with a simple imaging box. Results: The GFP-expressing orthotopic bladder tumor was imaged in realtime non-invasively as well as intra-vitally, with the two methods correlating at r=0.99. Conclusion: This is the first noninvasive-fluorescence-imaging orthotopic model of bladder cancer and can be used for rapidly screening novel effective agents for this recalcitrant disease. Materials and MethodsMice. Female nude nu/nu mice 6-8 weeks (AntiCancer, Inc., San Diego, CA, USA) were used. An inspection was performed to ensure their suitability for the study before cancer-cell implantation. The animals were maintained in a HEPA-filtered environment in a Micro-VENT full-ventilation rodent housing system (Allentown 3225This article is freely accessible online.

Oral Recombinant Methioninase Sensitizes a Bladder Cancer Orthotopic Xenograft Mouse Model to Low-dose Cisplatinum and Prevents Metastasis

“…In the present study, we used a GFP-expressing orthotopic bladder-cancer mouse model, in which tumor growth and especially metastasis can be imaged non-invasively and rapidly (26). In the present study, we demonstrated that o-rMETase can significantly enhance the inhibition of lowdose CDDP on primary tumor growth, cancer-cell proliferation and metastasis of bladder cancer.…”

“…Our previous studies have shown that r-METase combined with CDDP had a synergistic inhibition efficacy on primary tumor growth and metastasis in a variety of cancers, but this has not been reported in bladder cancer (19,(21)(22)(23)(24)(25). We previously established an imageable GFP-expressing orthotopic nudemouse model of human bladder cancer (26). In the present study, we evaluated the inhibition efficacy of low-dose CDDP in combination with o-rMETase on the primary tumor and metastasis of the orthotopic bladder-cancer model.…”

MicroRNA Expression Deregulated by Aberrant Methylation in B-ALL Mouse Model

“…These result suggest that the leukemia cells not only proliferate in the blood but also in ltrate the spleen. Among the reported uorescent tracer tumor models, the uorescent tracers commonly used to label tumor cells are red uorescent protein (RFP), GFP and CM-DiI uorescent dyes [16][17][18][19][20]. After transfected into cells by viral vectors, RFP or GFP genes can be randomly integrated into the chromosome of tumor cells with a long and stable expression characteristic.…”

“…After transfected into cells by viral vectors, RFP or GFP genes can be randomly integrated into the chromosome of tumor cells with a long and stable expression characteristic. Researchers have used this method to observe the invasion and metastasis of bladder cancer, esophageal squamous cell carcinoma, and pancreatic cancer cells in vivo [16][17][18]20]. CM-DiI uorescent dye can be directly added to the cell culture medium to combine with the glutathione in the cell to stain the cytoplasm red, which also has the characteristic of long-term non-decolorization.…”