A non-invasive method to isolate the neuronal linage from the nasal epithelium from schizophrenic and bipolar diseases

Benítez‐King, Gloria; Riquelme, Agustín; Ortíz-López, Leonardo; Berlanga, Carlos; Rodríguez-Verdugo, M.S.; Romo, F.; Calixto, Eduardo; Solís‐Chagoyán, Héctor; Jiménez, Mariela; Montaño, Luis M.; Ramírez-Rodríguez, Gerardo; Morales-Mulia, Sandra; Domínguez-Alonso, Aline

doi:10.1016/j.jneumeth.2011.07.009

Cited by 68 publications

(99 citation statements)

References 53 publications

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“…Participants were recruited from the Schizophrenia and Bipolar Disorder Clinics of this institution as described (Benítez-King et al, 2011). Experiments were conducted with the understanding and written consent of each participant and the study was previously approved by the Institute's Ethics Committee.…”

Section: Methodsmentioning

confidence: 99%

“…Exfoliates of nasal mucosa were obtained from four patients diagnosed with SZ, four patients diagnosed with BD, and from four subjects with good health conditions and without any medication (HS). Patients' cells were isolated by nose cavity exfoliation following the procedure described in detail by Benítez-King et al (2011) and were cultured up to five passages.…”

Section: Methodsmentioning

confidence: 99%

“…Cells with typical morphology of olfactory sensory neuron previously characterized by olfactory marker protein staining were selected for recording as described in detail by Benítez-King et al (2011). Briefly, pipette-electrodes (5-10 MΩ) were used for recording and all experiments were performed at approximately 20°C.…”

Section: Methodsmentioning

confidence: 99%

“…In this regard, we recently developed a non-invasive method to obtain viable olfactory neuroepithelial cells by nasal exfoliation from ambulatory patients and under culture conditions that promote neuronal progenitor-cell proliferation and their spontaneous morphofunctional polarization and differentiation into olfactory sensory neurons (Benítez-King et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Microtubule organization and L-type voltage-activated calcium current in olfactory neuronal cells obtained from patients with schizophrenia and bipolar disorder

Solís‐Chagoyán

Calixto

Figueroa

et al. 2013

Schizophrenia Research

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microtubule organization and L-type voltage-activated calcium current in olfactory neuronal cells obtained from patients with schizophrenia and bipolar disorder

Solís‐Chagoyán

Calixto

Figueroa

et al. 2013

Schizophrenia Research

Self Cite

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“…62 Las células de linaje neuronal criopreservadas en bancos que posteriormente se descongelaron y se volvieron a cultivar, conservaron sus propiedades electrofisiológicas y fenotípicas 62 y fueron capaces de desarrollar los arreglos del citoesqueleto característicos de la neuritogénesis. 62 Estos datos sugieren que los precursores neuronales obtenidos de la cavidad nasal de los pacientes neuropsiquiátricos constituyen un modelo experimental adecuado para investigar la fisiopatología de la EA, los aspectos celulares y moleculares del neurodesarrollo tales como la diferenciación de las neuritas en axones y dendritas, así como diversas acciones farmacológicas sobre este proceso, y para la caracterización de biomarcadores de rasgo.…”

Section: 61unclassified

La melatonina como un factor promotor de la diferenciación neuronal: implicaciones en el tratamiento de las demencias

et al. 2013

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SUMMARYDementias are progressive and neurodegenerative neuropsychiatric disorders, with a high worldwide prevalence. These disorders affect memory and behavior, causing impairment in the performance of daily activities and general disability in the elders. Cognitive impairment in these patients is related to anatomical and structural alterations at cellular and sub-cellular levels in the Central Nervous System. In particular, amyloid plaques and neurofibrillar tangles have been defined as histopathological hallmarks of Alzheimer's disease. Likewise, oxidative stress and neuroinflammation are implicated in the etiology and progression of the disease.Neuronal precursors from human olfactory neuroepithelium have been recently characterized as an experimental model to identify neuropsychiatric disease biomarkers. Moreover, this model not only allows the study of neuropsychiatric physiopathology, but also the process of neurodevelopment at cellular, molecular and pharmacological levels.This review gathers the evidence to support the potential therapeutic use of melatonin for dementias, based on its antioxidant properties, its anti-inflammatory effect in the brain, and its ability to inhibit both tau hyper-phosphorylation and amyloid plaque formation. Furthermore, since melatonin stimulates neurogenesis, and promotes neuronal differentiation by inducing the early stages of neuritogenesis and dendrite formation, it has been suggested that melatonin could be useful to counteract the cognitive impairment in dementia patients.Key words: Melatonin, dementia, biomarkers, neuroepithelium, neuritogenesis. RESUMENLas demencias son enfermedades neuropsiquiátricas, progresivas, neurodegenerativas y con una alta prevalencia a nivel mundial. Ocupan uno de los primeros lugares como enfermedades que causan incapacidad en los adultos mayores. En estos pacientes el Sistema Nervioso Central presenta alteraciones anatómico-estructurales a nivel celular y subcelular que se asocian con deficiencias cognitivas. En particular, en la enfermedad de Alzheimer se han caracterizado marcadores histopatológicos como las placas amiloides y las marañas neurofibrilares. Se sabe que el estrés oxidativo y la neuroinflamación participan en la etiología y el desarrollo de la enfermedad. Recientemente se caracterizó a los precursores neuronales del neuroepitelio olfatorio humano como un modelo experimental adecuado para identificar biomarcadores de rasgo y para estudiar la fisiopatología de diversas enfermedades neuropsiquiátricas, así como el proceso del neurodesarrollo, a nivel celular, molecular y farmacológico. En este trabajo se presenta la evidencia que sustenta que la melatonina puede ser útil en el tratamiento de las demencias, por su capacidad antioxidante, por su efecto anti-inflamatorio, así como por el efecto inhibidor de la hiperfosforilación de la proteina tau y de la formación de placas amiloides. Además, al estimular la formación de nuevas neuronas, la neuritogénesis en sus etapas tempranas y la formación de dendritas, la melatonina podría...

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Progress Toward Therapies and Interventions for Neurodevelopmental Disorders

Ajetunmobi

Tropea

2015

The Genetics of Neurodevelopmental Disorders

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A non-invasive method to isolate the neuronal linage from the nasal epithelium from schizophrenic and bipolar diseases

Cited by 68 publications

References 53 publications

Microtubule organization and L-type voltage-activated calcium current in olfactory neuronal cells obtained from patients with schizophrenia and bipolar disorder

Microtubule organization and L-type voltage-activated calcium current in olfactory neuronal cells obtained from patients with schizophrenia and bipolar disorder

La melatonina como un factor promotor de la diferenciación neuronal: implicaciones en el tratamiento de las demencias

Progress Toward Therapies and Interventions for Neurodevelopmental Disorders

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