2016
DOI: 10.1111/jofo.12145
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A non-lethal biopsy technique for sampling subcutaneous adipose tissue of small and medium-sized birds

Abstract: Analysis of fatty acids from adipose tissue can provide important information about the physiological and nutritional condition of birds. However, non‐lethal biopsy procedures and their potential negative effects on small and medium‐sized birds have not been adequately assessed. We developed a biopsy procedure for collecting small amounts of adipose tissue from the furcular area of small and medium‐sized birds (13–62 g) without adverse effects. The biopsy procedure was performed on Dunlins (Calidris alpina), a… Show more

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Cited by 10 publications
(10 citation statements)
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“…We collected adipose tissue from the visible fat stores in the furcular area of starlings used in Experiment I ( n = 29) 5–7 days after their longest flight in the windtunnel. We conducted the biopsies of this furcular fat using the methods outlined in Rocha et al, 2016 . Briefly, upper breast feathers were wetted and moved aside to expose the skin and visible subcutaneous yellow-colored fat stores.…”
Section: Methodsmentioning
confidence: 99%
“…We collected adipose tissue from the visible fat stores in the furcular area of starlings used in Experiment I ( n = 29) 5–7 days after their longest flight in the windtunnel. We conducted the biopsies of this furcular fat using the methods outlined in Rocha et al, 2016 . Briefly, upper breast feathers were wetted and moved aside to expose the skin and visible subcutaneous yellow-colored fat stores.…”
Section: Methodsmentioning
confidence: 99%
“…All birds were sampled in early morning, 24 h post-injection for blood and visible subcutaneous fat according to Rocha et al . 35 . No godwit was kept for more than 48 h in fasting (Fig.…”
Section: Methodsmentioning
confidence: 99%
“…To the best of our knowledge, this was quantified for the first time in a free-ranging bird species, using 2 H 2 O as metabolic tracer, and by applying NMR-based techniques following the methodologies previously described for rodents (Duarte et al, 2014) and fish (Viegas et al, 2016). The localized biopsy method developed by Rocha et al (2016) allowed for sampling of subcutaneous fat in the furcular zone that provides the bulk of the lipid fuel for these birds. Even if it was not possible to trace the changes in other regional adipose stores, this method allowed the experimental procedures to be performed without observable signs of distress on the birds during the short period held in captivity, resulting in their subsequent successful release into the wild.…”
Section: Discussionmentioning
confidence: 99%
“…Plasma was stored at −20°C until analysis of body water 2 H-enrichments by 2 H nuclear magnetic resonance (NMR) analysis, quantification of plasma glucose (glucose oxidase method, using a glucose analyser-YSI Model 1500 Sport) and quantification of plasma triglycerides (Spinreact, ref: 1001314). Subcutaneous fat biopsies (∼13.0± 1.5 mg, n=12; Table 2) were obtained by a small incision in the furcular zone according to Rocha et al (2016). This area consists primarily of loose connective tissue and subcutaneous yellow fat tissue which was clearly visible under the skin and accessible after laterally moving the feathers from the breast area.…”
Section: Materials and Methods Bird Capture And Experimental Setupmentioning
confidence: 99%