A non-lethal SPME method for insect cuticular analysis by GC-MS

Ferreira-Caliman, M. J.; Andrade-Silva, Aline Candida Ribeiro; Guidetti-Campos, Maria Cláudia; Turatti, Izabel Cristina Casanova; Nascimento, Fábio Santos do; Lopes, Norberto Peporine

doi:10.1039/c4ay01909a

Cited by 11 publications

(9 citation statements)

References 40 publications

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“…However, there are some problems with the commercially available SPME fibers (Supelco, Bellefonte, PA, USA): a) they are expensive, b) repeated use could cause damage to the coating, and c) they have to be analyzed relatively quickly compared to other methods [ 35 , 36 ]. In addition, d) long GC analysis times limit the use of SPME fibers for collecting and analyzing large numbers of samples in a short amount of time, unless the fibers are extracted in organic solvent and reused [ 35 ]. Therefore, direct-contact SPME is not widely applied and has only been used for large sample sizes (N > 50) in a small number of studies [ 26 , 28 , 29 , 31 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

“…Disposable usage of these fused silica optical fibers for sampling pheromone glands costs a fraction of the original material costs of SPME. Furthermore, analytes can be desorbed from the fiber using an organic solvent and stored until GC-analysis [ 35 ], without the need to worry about damaging the coating. This has the additional advantage that the same sample can be used for multiple analyses such as GC-EAD, GC-MS, or derivatization.…”

Section: Introductionmentioning

confidence: 99%

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Disposable Polydimethylsiloxane (PDMS)-Coated Fused Silica Optical Fibers for Sampling Pheromones of Moths

Lievers

Groot

2016

PLoS ONE

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In the past decades, the sex pheromone composition in female moths has been analyzed by different methods, ranging from volatile collections to gland extractions, which all have some disadvantage: volatile collections can generally only be conducted on (small) groups of females to detect the minor pheromone compounds, whereas gland extractions are destructive. Direct-contact SPME overcomes some of these disadvantages, but is expensive, the SPME fiber coating can be damaged due to repeated usage, and samples need to be analyzed relatively quickly after sampling. In this study, we assessed the suitability of cheap and disposable fused silica optical fibers coated with 100 μm polydimethylsiloxane (PDMS) by sampling the pheromone of two noctuid moths, Heliothis virescens and Heliothis subflexa. By rubbing the disposable PDMS fibers over the pheromone glands of females that had called for at least 15 minutes and subsequently extracting the PDMS fibers in hexane, we collected all known pheromone compounds, and we found a strong positive correlation for most pheromone compounds between the disposable PDMS fiber rubs and the corresponding gland extracts of the same females. When comparing this method to volatile collections and the corresponding gland extracts, we generally found comparable percentages between the three techniques, with some differences that likely stem from the chemical properties of the individual pheromone compounds. Hexane extraction of cheap, disposable, PDMS coated fused silica optical fibers allows for sampling large quantities of individual females in a short time, eliminates the need for immediate sample analysis, and enables to use the same sample for multiple chemical analyses.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Disposable Polydimethylsiloxane (PDMS)-Coated Fused Silica Optical Fibers for Sampling Pheromones of Moths

Lievers

Groot

2016

PLoS ONE

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“…In our limited sample, levels of grooming upon reintroduction were elevated for treatment individuals, but not significantly so ( p = 0.07; table 1). To test this hypothesis further will require experiments using non-destructive CHC sampling techniques (such as the use of solid phase microextraction (SPME) fibres [41]) to determine whether the profile of excluded individuals drifts from that of their native colony over time; and whether allogrooming functions to homogenize the CHC profile of intruders.…”

Section: Discussionmentioning

confidence: 99%

Lack of aggression and apparent altruism towards intruders in a primitive termite

et al. 2016

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In eusocial insects, the ability to discriminate nest-mates from non-nest-mates is widespread and ensures that altruistic actions are directed towards kin and agonistic actions are directed towards non-relatives. Most tests of nest-mate recognition have focused on hymenopterans, and suggest that cooperation typically evolves in tandem with strong antagonism towards non-nest-mates. Here, we present evidence from a phylogenetically and behaviourally basal termite species that workers discriminate members of foreign colonies. However, contrary to our expectations, foreign intruders were the recipients of more rather than less cooperative behaviour and were not subjected to elevated aggression. We suggest that relationships between groups may be much more peaceable in basal termites compared with eusocial hymenoptera, owing to energetic and temporal constraints on colony growth, and the reduced incentive that totipotent workers (who may inherit breeding status) have to contribute to self-sacrificial intergroup conflict.

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“…This non-lethal SPME-based platform can be potentially introduced into toxicological assessment of volatile organic compounds [76]. An alternative non-lethal approach to determination of cuticular hydrocarbons involved DI-SPME extraction by gently rubbing the exposed fiber against ants [77]. Many of the lower vertebrates, including fish, metabolize chemicals using pathways similar to human pathways.…”

Section: Invertebrates Lower Vertebrates and Microorganismsmentioning

confidence: 99%

SPME as a promising tool in translational medicine and drug discovery: From bench to bedside

Goryński

Goryńska

Górska

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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A non-lethal SPME method for insect cuticular analysis by GC-MS

Abstract: In the present study a new method involving extraction by SPME fibers and storage in an organic solvent is described and tested in an ant species,Dinoponera quadriceps.

Cited by 11 publications

References 40 publications

Disposable Polydimethylsiloxane (PDMS)-Coated Fused Silica Optical Fibers for Sampling Pheromones of Moths

Disposable Polydimethylsiloxane (PDMS)-Coated Fused Silica Optical Fibers for Sampling Pheromones of Moths

Lack of aggression and apparent altruism towards intruders in a primitive termite

SPME as a promising tool in translational medicine and drug discovery: From bench to bedside

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