A non-pathogenic strain of Fusarium oxysporum as a potential biocontrol agent against Verticillium wilt of olive

Mulero-Aparicio, Antonio; Agustí-Brisach, Carlos; Varo, A.; López-Escudero, Francisco Javier; Trapero, A.

doi:10.1016/j.biocontrol.2019.104045

Cited by 33 publications

(42 citation statements)

References 33 publications

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“…The activity in vitro was higher in liquid than in solid medium, which has also been observed against other phytopathogenic fungi, such as Botrytis cinerea [34,49]. Similar inhibition rates against V. dahliae were observed in plate assays with B. thuringiensis isolates [70] and a non-pathogenic strain of Fusarium oxysporum [25]. Regarding the antifungal activity of the supernatant, this indicates that the activity is due to extracellular metabolites and is not only contact-dependent.…”

Section: Discussionsupporting

confidence: 74%

“…To date, selected isolates of several fungal species have been described as biological control agents against Verticillium. For instance, Trichoderma asperellum [ 24 ] and Fusarium oxysporum [ 25 ] have been identified as potential tools to fight Verticillium wilt. Concerning bacterial biocontrol agents, Serratia plymuthica [ 26 ], Paenibacillus alvei [ 27 ], and Pseudomonas fluorescens [ 28 ] have proved to be effective against V. dahliae in plant experiments.…”

Section: Introductionmentioning

confidence: 99%

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Biological Control of Verticillium Wilt on Olive Trees by the Salt-Tolerant Strain Bacillus velezensis XT1

Castro¹,

Torres

Sampedro

et al. 2020

Microorganisms

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Verticillium wilt, caused by the pathogen Verticillium dahliae, is extremely devastating to olive trees (Olea europea). Currently, no successful control measure is available against it. The objective of this work was to evaluate the antifungal activity of Bacillus velezensis XT1, a well-characterized salt-tolerant biocontrol strain, against the highly virulent defoliating V. dahliae V024. In vitro, strain XT1 showed to reduce fungal mycelium from 34 to 100%, depending on if the assay was conducted with the supernatant, volatile compounds, lipopeptides or whole bacterial culture. In preventive treatments, when applied directly on young olive trees, it reduced Verticillium incidence rate and percentage of severity by 54 and ~80%, respectively. It increased polyphenol oxidase (PPO) activity by 395%, indicating an enhancement of disease resistance in plant tissues, and it decreased by 20.2% the number of fungal microsclerotia in soil. In adult infected trees, palliative inoculation of strain XT1 in the soil resulted in a reduction in Verticillium symptom severity by ~63%. Strain XT1 is biosafe, stable in soil and able to colonize olive roots endophytically. All the traits described above make B. velezensis XT1 a promising alternative to be used in agriculture for the management of Verticillium wilt.

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Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Biological Control of Verticillium Wilt on Olive Trees by the Salt-Tolerant Strain Bacillus velezensis XT1

Castro¹,

Torres

Sampedro

et al. 2020

Microorganisms

View full text Add to dashboard Cite

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“…Likewise, treatments with conidial suspensions or chlamydospores of FO12 were effective in reducing both the inoculum density of V. dahliae in the soil and the VWO severity [169]. As for the biocontrol mechanism involved, the effect of secondary metabolites produced by FO12 against V. dahliae was investigated [169,170]. In vitro experiments indicated that the metabolites produced by FO12 had little effect on the inhibition of V. dahliae mycelial growth, suggesting that the antagonistic effect relies on the presence of FO12 alive and on a direct interaction with the pathogen [169].…”

Section: The Continuous Search For Effective Biological Control Agentmentioning

confidence: 97%

“…This isolate also showed the ability to reduce symptoms in "Picual" plants under controlled conditions [168]. Likewise, treatments with conidial suspensions or chlamydospores of FO12 were effective in reducing both the inoculum density of V. dahliae in the soil and the VWO severity [169]. As for the biocontrol mechanism involved, the effect of secondary metabolites produced by FO12 against V. dahliae was investigated [169,170].…”

Section: The Continuous Search For Effective Biological Control Agentmentioning

confidence: 99%

“…As for the biocontrol mechanism involved, the effect of secondary metabolites produced by FO12 against V. dahliae was investigated [169,170]. In vitro experiments indicated that the metabolites produced by FO12 had little effect on the inhibition of V. dahliae mycelial growth, suggesting that the antagonistic effect relies on the presence of FO12 alive and on a direct interaction with the pathogen [169]. In addition, the production of VOC by FO12 in the presence of V. dahliae has been reported.…”

Section: The Continuous Search For Effective Biological Control Agentmentioning

confidence: 99%

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Verticillium Wilt of Olive and Its Control: What Did We Learn during the Last Decade?

Montes-Osuna

Mercado‐Blanco

2020

Plants

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Verticillium (Verticillium dahliae Kleb.) wilt is one of the most devastating diseases affecting olive (Olea europaea L. subsp. europaea var. europaea) cultivation. Its effective control strongly relies on integrated management strategies. Olive cultivation systems are experiencing important changes (e.g., high-density orchards, etc.) aiming at improving productivity. The impact of these changes on soil biology and the incidence/severity of olive pests and diseases has not yet been sufficiently evaluated. A comprehensive understanding of the biology of the pathogen and its populations, the epidemiological factors contributing to exacerbating the disease, the underlying mechanisms of tolerance/resistance, and the involvement of the olive-associated microbiota in the tree’s health is needed. This knowledge will be instrumental to developing more effective control measures to confront the disease in regions where the pathogen is present, or to exclude it from V. dahliae-free areas. This review compiles the most recent advances achieved to understand the olive–V. dahliae interaction as well as measures to control the disease. Aspects such as the molecular basis of the host–pathogen interaction, the identification of new biocontrol agents, the implementation of “-omics” approaches to unravel the basis of disease tolerance, and the utilization of remote sensing technology for the early detection of pathogen attacks are highlighted.

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Use of natural‐based commercial products as an alternative for providing bioprotection against verticillium wilt of olive

Antón‐Domínguez,

Díaz‐Díaz,

Acedo‐Antequera

et al. 2024

J Sci Food Agric

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BACKGROUNDDue to the ineffectiveness of existing control methods against Verticillium dahliae, the causal agent of Verticillium wilt of olive (Olea europaea; VWO), it is necessary to search for sustainable and environmentally friendly alternatives such as bioprotection by products based on plant extracts and other naturally synthesized compounds. Therefore, the main objective of this study was to evaluate the effects of seven natural‐based commercial products on the inhibition of mycelial growth, the germination of V. dahliae conidia and microsclerotia, and disease progression in olive plants (cv. Picual). Aluminium lignosulfonate and a copper phosphonate salt (copper phosphite) were included for comparative purposes.RESULTSThe seaweed and willow extracts and copper phosphite inhibited V. dahliae mycelial growth by more than 50% at the high doses tested. Most of the products inhibited conidial germination up to 90% compared to the control at the high doses tested. However, none of the products showed efficacy above 50% in inhibiting microsclerotia (MS) germination. The willow extract was the most effective at reducing disease severity and progression in olive plants, with no significant differences compared to the noninoculated negative control.CONCLUSIONSThe results of this study suggest that the use of natural‐based products, i.e., seaweed and willow extracts, is a potential sustainable alternative in an integrated VWO control strategy.This article is protected by copyright. All rights reserved.

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A non-pathogenic strain of Fusarium oxysporum as a potential biocontrol agent against Verticillium wilt of olive

Cited by 33 publications

References 33 publications

Biological Control of Verticillium Wilt on Olive Trees by the Salt-Tolerant Strain Bacillus velezensis XT1

Biological Control of Verticillium Wilt on Olive Trees by the Salt-Tolerant Strain Bacillus velezensis XT1

Verticillium Wilt of Olive and Its Control: What Did We Learn during the Last Decade?

Use of natural‐based commercial products as an alternative for providing bioprotection against verticillium wilt of olive

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