A non-radioactive PCR-SSCP analysis allows to distinguish between HPV 16 European and Asian-American variants in squamous cell carcinomas of the uterine cervix in Colombia

Moreno‐Acosta, Pablo; Molano, Mónica; Huertas, António Delgado; Gómez, Manuel Iván; Romero, Alfredo; González, Mauricio; Bravo, María Mercedes; García‐Carrancá, Alejandro

doi:10.1007/s11262-008-0239-8

Cited by 11 publications

(9 citation statements)

References 46 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Identification of HPV variants relies on sequencing or hybridization with specific probes of amplified fragments of the LCR, E6 or L1 genes, 26 ranging in size [27][28][29] from 240 bp to 1.5 kb. Amplification of fragments of such size requires good quality template DNA and is usually not possible in formalin-fixed paraffin-embedded biopsies.…”

Section: Discussionmentioning

confidence: 99%

Clinical evaluation of polymerase chain reaction reverse hybridization assay for detection and identification of human papillomavirus type 16 variants

Sánchez

Kleter

Gheit

et al. 2011

Journal of Clinical Virology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Clinical evaluation of polymerase chain reaction reverse hybridization assay for detection and identification of human papillomavirus type 16 variants

Sánchez

Kleter

Gheit

et al. 2011

Journal of Clinical Virology

View full text Add to dashboard Cite

“…Therefore, HPV-16 variants could also be biomarkers of radioresistance and anti-viral drugs might act as agents restoring radio-sensitivity [27]. Even if HPV was not assessed in the present study’s population, the probability of HPV infection was high in our set of patients, since most of Colombian CC are HPV-positive [28, 29]. …”

Section: Discussionmentioning

confidence: 95%

Biomarkers of resistance to radiation therapy: a prospective study in cervical carcinoma

et al. 2017

View full text Add to dashboard Cite

BackgroundClinical parameters and proteins have recently been suggested as possible causes of radiotherapy (RT) resistance in cervical carcinoma (CC). The objective of the present study was to validate prognostic biomarkers of radiation resistance.MethodsThe present prospective study included patients undergoing RT with curative intent for histologically proven locally advanced squamous cell CC. Tissues and blood samples were systematically collected before RT initiation. Immuno-histochemistry was performed (IGF-IR α and β, GAPDH, HIF-1 alpha, Survivin, GLUT1, CAIX, hTERT and HKII). Response to radiation was assessed through tumour response 3 months after RT completion, through overall survival (OS) and through progression-free survival (PFS).ResultsOne hundred forty nine patients with a mean age of 46 years were included, with FIGO IIB (n = 53) and FIGO IIIB (n = 96) CCs. 61 patients were treated with exclusive RT + brachytherapy and 88 underwent chemo-radiotherapy + brachytherapy. Our findings suggest an association between hemoglobin level (Hb) (>11 g/dL) and 3 months complete response (p = 0.02). Hb level < 11 g/dL was associated with decreased PFS (p = 0.05) and OS (p = 0.08). Overexpression of IGF-1R β was correlated with a decreased OS (p = 0.007). Overexpression of GLUT1 was marginally correlated with reduced OS (p = 0.05). PFS and OS were significantly improved in patients undergoing chemoradiation versus exclusive radiotherapy (PFS: p = 0.04; OS: p = 0.01).ConclusionsIGF-1R β overexpression and Hb level (≤11 g/dl) were associated with poor prognosis, and thus appear to be possible interesting biomarkers of radiation resistance. Our results corroborate previous pre-clinical studies suggesting IGF-1R and hypoxia to be part of the biological pathways leading to radio-resistance.

show abstract

“…The neoplastic progression or transformation of preneoplastic lesions in invasive cancer cells is provoked at least partly by HPV, especially through gene and protein expression modulation [ 2 , 5 , 6 , 10 ]. This modulation involves oncogene activation, loss of tumor suppressor genes (mostly p53) and alterations in molecular signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

“…Protein expression modulation can be considered as a promising hypothetical model. It may refer to characteristic of the tumor microenvironment, such as hypoxia (low oxygen levels up to 1%), increased glycolysis, and acidosis (low pH) [ 10 – 13 ]. Indeed, the malignant cells use alternative metabolic pathways to develop and survive [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…The results of our studies showed that response to treatment, gene expression, and protein expression in the presence of high-risk HPV and variants of HPV-16 were linked. Through these studies, we contributed to the identification of predictive and prognostic biomarkers [ 10 – 15 ]. Recent analyses have aimed at improving the assessment of patients' individual risks [ 16 – 18 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Persistent High-Risk HPV Infection and Molecular Changes Related to the Development of Cervical Cancer

Moreno‐Acosta

Romero‐Rojas

Vial

et al. 2020

Case Reports in Obstetrics and Gynecology

Self Cite

View full text Add to dashboard Cite

This article is a preliminary investigational study that is aimed at giving hints about the interesting biomarkers involved in the transition process from low-grade cervix lesion to invasive cervical cancer. Our study focuses on the risk factors and tumour molecular changes in one patient. First in 1986, she was diagnosed a preinvasive cervix lesion. Then, 16 years later, she was diagnosed an invasive cervical cancer. The 2002 diagnosis was a squamous cell carcinoma of the cervix, stage IIIB (FIGO), whereas in 1986, she had been diagnosed a high-grade squamous intraepithelial cervical lesion. Retrospectively, the analysis of samples of preneoplastic lesions and invasive cervical cancer confirmed the histopathological diagnoses and detected the presence of HPV type and HPV-16 variants, as well as the overexpression of proteins such as hTERT, IGF1Rα, IGF1Rβ, CAIX, and GLUT1. Finally, the Arg72Pro polymorphism was detected in TP53. The role of high-risk HPV and HPV-16 variants and of hTERT, IGF1Rα, IGF1Rβ, CAIX, and GLUT1 variations seemed confirmed in the development and progression of cervical cancer. As a result, analyzing the molecular changes in one and same tumour that progresses from a low-grade cervix lesion to invasive cervical cancer could provide valuable information in order to improve detection, diagnosis, and treatment in the future.

show abstract

A non-radioactive PCR-SSCP analysis allows to distinguish between HPV 16 European and Asian-American variants in squamous cell carcinomas of the uterine cervix in Colombia

Cited by 11 publications

References 46 publications

Clinical evaluation of polymerase chain reaction reverse hybridization assay for detection and identification of human papillomavirus type 16 variants

Clinical evaluation of polymerase chain reaction reverse hybridization assay for detection and identification of human papillomavirus type 16 variants

Biomarkers of resistance to radiation therapy: a prospective study in cervical carcinoma

Persistent High-Risk HPV Infection and Molecular Changes Related to the Development of Cervical Cancer

Contact Info

Product

Resources

About