A non-sterile heterotrophic microalgal process for dual biomass production and carbon removal from swine wastewater

Microalgal Growth and Nutrient Removal Efficiency in Non-Sterilised Primary Domestic Wastewater

“…In Walls et al study [35], Scenedesmus sp., cultivated in non-sterilised undiluted domestic wastewater, achieved a removal rate of only 60% for nitrate, 53% for N-NH 4 , and 46% for orthophosphate. In another study conducted by Agustin et al [68], the microalgae consortium removed 86.2% N-NH 4 and 4.4% orthophosphate from non-sterilised domestic wastewater. The nutrient removal efficiency of C. aquaticum and Scenedesmus sp.…”

Direct biohydrogen production from chitosan harvested microalgae biomass and an isolated yeast

“…The microalgae consortium used in this study consisted of Scenedesmus obliquus and Scenedesmus sp. predominantly, which were found in a wastewater plant in Mexico City from a pilot scale mode of microalgae cultivation ("Atzintli"), constructed by the Engineering Institute of UNAM, Mexico and Newcastle University, UK [33]. The microalgae consortia were previously adapted and growth with a synthetic culture broth (BG-11 Media).…”

Future bioenergy source by microalgae–bacteria consortia: a circular economy approach

“…147 Microalgal–bacterial consortia offer an alternative approach by employing non-sterile microalgae heterotrophic processes to treat wastewater with high concentrations of organic matter, leading to reduced operational demands such as illuminated cultivation areas compared to phototrophic cultures reliant on light as well as the requirement for an external organic substrate. 145…”

“…144 In general, the incorporation of organic substrates and the need for sterilization to counteract resource competition from other microorganisms in wastewater pose a constraint in adopting heterotrophic cultures for wastewater treatment and biomass generation, with the associated expenses rendering the process economically unfeasible. 145 Glucose has been introduced as an organic carbon source in most heterotrophic cultures documented in the existing literature. For example, cultivating C. vulgaris in wastewater supplemented with glucose and sodium acetate (NaAc) resulted in high biomass, lipid, and carbohydrate productivities, surpassing those achieved under photoautotrophic conditions.…”

“…147 Microalgal-bacterial consortia offer an alternative approach by employing non-sterile microalgae heterotrophic processes to treat wastewater with high concentrations of organic matter, leading to reduced operational demands such as illuminated cultivation areas compared to phototrophic cultures reliant on light as well as the requirement for an external organic substrate. 145 Despite several benefits associated with suspended microalgal-bacterial consortia, challenges persist because of the complexities involved in effectively separating and harvesting dispersed microalgal biomass to achieve satisfactory effluent quality. Moreover, the low biomass concentration of microalgae in the culture medium and the small individuals require complex biochemical engineering operations to enrich and harvest the microalgae biomass, subsequently increasing the production cost of microalgae biomass.…”