A nonhomologous end-joining mutant forNeurospora sitophilaresearch

Abstract

Disruption of the nonhomologous end-joining (NHEJ) pathway has been shown to increase the efficiency of transgene integration into targeted genomic locations of Neurospora crassa and other fungi. Here, we report that a similar phenomenon occurs in a second Neurospora species: N. sitophila. Specifically, we show that deletion of N. sitophila mus-51 increases the efficiency of targeted-transgene integration, presumably by disrupting NHEJ. Researchers interested in obtaining the N. sitophila mus-51 ∆ strains described in this study can obtain them from the Fungal Genetics Stock Center (FGSC).

RNA editing controls meiotic drive by a Neurospora Spore killer

“…Transformation of N. crassa was performed by the electroporation method of Margolin et al (1997) with modifications as described by Rhoades et al (2020). Strains ISU-4954 and ISU-4955 were obtained by transformation of strain P8-43 with plasmids pNR154.4 and pNR157.6, respectively.…”