A Noninvasive Delivery of DNA into Zygotes by AuNPs−PEI for Transgenic Mice

Abstract

A transgenic system of noninvasive cytosolic transport based on exogenous plasmid DNA (pIRES2À EGFP) loading on gold nanoparticles (AuNPs) coated with polyethyleneimine (PEI) was successfully constructed, which can remove the technical threshold and possible damage of microinjection to oocytes and embryo development. In this study, mouse zygotes were cultured in KSOM medium containing the complex of AuNPsÀ PEIÀ DNA (APD) until formation of blastocyts, and then transferred into pseudopregnant female mice for obtaining transgenic offspring. Fluorescence observations confirm the expression of GFP in early embryos; importantly, data from identifying experiments by genotyping, immunofluorescence and DNA sequencing show that the GFP sequence indeed integrates into the chromosome of offspring individuals and is expressed. Furthermore, TUNEL test and analysis on cell differentiation of blastocysts indicate an excellent safety of APD to the develop

RETRACTED: AgNPs reduce reproductive capability of female mouse for their toxic effects on mouse early embryo development

“…AuNPs of 5-10 nm were obtained by the method as described in the earlier study. 23 In brief, 0.05% polyethyleneimine (PEI) solution with a molecular weights of 25, 000 and 1800 was mixed in an equal volume ratio, then 700 μL of fresh 2% HAuCl4 solution was added into 16 mL of freshly prepared PEI solution, and the final volume was made to 22 mL with ultrapure water. Then, at room temperature, the mixture was stirred at 800 rpm/min for 48 h, filtered with a membrane of 0.22 μm pore size, and stored at 4°C.…”